Thermo labsystems multiskan rc

The RAW 264.7, J774A.1, NIH/3T3, MC38/0, HT-29, and T98G cells were placed in 96-well plates (Corning Costar, Corning, NY, USA) at a density of 5 × 10³ cells/well and JAWS II and THP-1 cells at a density of 1 × 10⁴ cells/well. After 24 h, the aqueous suspensions of boron carbide preparations (B₄C 1, B₄C 2) and BPA ((L)-4-dihydroxy-borylphenylalanine) (Sigma-Aldrich) were diluted in the culture medium (appropriate for each cell) to neutralize the pH and were added at various concentrations (in the range from 0.1 to 400 µg/mL) to the plates with the cells. The cells were incubated with the compounds for 72 h. Next, the MTT dye (3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide; 5 mg/mL) (Sigma-Aldrich) was added for 4 h. After this time, the cells were lysed overnight in a lysis buffer (N,N-dimethylmethanamide, sodium dodecyl sulfate, and water) at 37 °C. The absorbance value was recorded at 570 nm using a Thermo Labsystems Multiskan RC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA, USA) with Genesis Lite 3.05 Software (Thermo Life Sciences). The IC₅₀ values were calculated using GraphPad Prism 9 software (GraphPad Software, La Jolla, CA, USA).

The RAW 264.7, J774A.1, NIH/3T3, MC38/0, HT-29, and T98G cells were placed in 96-well plates (Corning Costar, Corning, NY, USA) at a density of 5 × 10³ cells/well and JAWS II and THP-1 cells at a density of 1 × 10⁴ cells/well. After 24 h, the aqueous suspensions of boron carbide preparations (B₄C 1, B₄C 2) and BPA ((L)-4-dihydroxy-borylphenylalanine) (Sigma-Aldrich) were diluted in the culture medium (appropriate for each cell) to neutralize the pH and were added at various concentrations (in the range from 0.1 to 400 µg/mL) to the plates with the cells. The cells were incubated with the compounds for 72 h. Next, the MTT dye (3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide; 5 mg/mL) (Sigma-Aldrich) was added for 4 h. After this time, the cells were lysed overnight in a lysis buffer (N,N-dimethylmethanamide, sodium dodecyl sulfate, and water) at 37 °C. The absorbance value was recorded at 570 nm using a Thermo Labsystems Multiskan RC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA, USA) with Genesis Lite 3.05 Software (Thermo Life Sciences). The IC₅₀ values were calculated using GraphPad Prism 9 software (GraphPad Software, La Jolla, CA, USA).