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Gipz non silencing lentiviral shrna control

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The GIPZ Non-silencing Lentiviral shRNA Control is a commercial product designed for use in molecular biology and cell biology research applications. It serves as a control for experiments involving lentiviral-mediated shRNA delivery and gene silencing.

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Lab products found in correlation

Prl tk vector, by Promega (1 mentions) Pgl3 luciferase reporter vector, by Promega (1 mentions)

Spelling variants of this product

Control shRNA (17 citations) GIPZ lentiviral shRNA (2 citations) GIPZ-control shRNA (2 citations)

2 protocols using gipz non silencing lentiviral shrna control

1

Targeting Human OCT3 with shRNA

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Four shRNA constructs targeting human OCT3 were obtained from Open Biosystems. The 21 bp targeting sequences were: 5′-AAGAATAAGTGTGCATTTC-3′ (clone ID V2LHS_98079), 5′-TTTAGGGTGACAGTGGAGG-3′ (V2LHS_232514), 5′-TTGATGAGAGGTATTTCCC-3′ (V3LHS_371553), 5′-ATTTCTGTCACAATCACGT-3′ (V3LHS_371552). GIPZ Non-silencing Lentiviral shRNA Control was also obtained from Open Biosystems. See Supplementary Materials for additional details on virus production and transduction.
Madera D., Vitale-Cross L., Martin D., Schneider A., Molinolo A.A., Gangane N., Carey T.E., McHugh J.B., Komarck C.M., Walline H.M., William WN J.r., Seethala R.R., Ferris R, & Gutkind J.S. (2015). Prevention of tumor growth driven by PIK3CA and HPV oncogenes by targeting mTOR signaling with metformin in oral squamous carcinomas expressing OCT3. Cancer prevention research (Philadelphia, Pa.), 8(3), 197-207.
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2

Investigating NF-kB Pathway Modulation

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The following shRNAs were obtained from Open Biosystems: GIPZ Non-silencing Lentiviral shRNA Control, GIPZ Human NFKBIA shRNA D2 and shRNA D7. The following shRNA was obtained from Sigma: human PLKO.1 pure derived vector RelA. The following plasmid were obtained from Addgene: RelA cFlag pcDNA3 plasmid, pCMV-VSV-G, pCMV-dR8.2 dvpr, p1242-3x-KB-L. IκBα was cloned into myc-tagged pRK5 plasmid. The following plasmid were obtained from Promega: pRL-TK Vector and pGL3 Luciferase Reporter Vectors.
Carrà G., Ermondi G., Riganti C., Righi L., Caron G., Menga A., Capelletto E., Maffeo B., Lingua M.F., Fusella F., Volante M., Taulli R., Guerrasio A., Novello S., Brancaccio M., Piazza R, & Morotti A. (2021). IκBα targeting promotes oxidative stress-dependent cell death. Journal of Experimental & Clinical Cancer Research : CR, 40, 136.
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