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3 protocols using dichloromethane dcm

1

Synthesis and Characterization of MWCNT-Polymer Composites

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Multiwalled Carbon nanotubes (CNTs) were purchased from Hanwha Nanotech Co. (Republic of Korea). The length and outer diameter of MWCNTs were found to be 10–20 μm and 10–30 nm, respectively with average purities more than 95%. Poly (methyl methacrylate) (PMMA-IG 840) and Polyvinylidene fluoride (PVDF-6008/001 Solef) were purchased from Solvay, India and LG Chemical, respectively. Kapton film was purchased from Jay Industries, Mumbai, India; concentrated sulphuric acid (H2SO4) of 98% purity, Ether of 98% purity and dichloromethane (DCM) of 99% purity were purchased from Himedia laboratories Pvt. Ltd. Mumbai, India; potassium permanganate (KMnO4) of 99% purity was purchased from Rankem, RFCL Ltd. New Delhi, India; hydrogen peroxide (H2O2) of 30% purity was purchased from Loba Chemie Pvt. Ltd. Mumbai, India; dimethylformamide (DMF) of 99% purity was purchased from Avantor, Maharashtra, India and Al membrane was purchased from local market.
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2

Preparation and Evaluation of Gefitinib-Loaded PLA/PVA Nanoparticles

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Poly (lactic acid) (PLA) (MW 2 ∗ 104 g/mol) was purchased from Sigma Aldrich, Poly (vinyl alcohol) (PVA) (MW86.09 g/mol), Dichloromethane (DCM) (MW 84.93) and Poly (ethylene glycol) (PEG) (MW 62.068 g/mol) were taken from Himedia Laboratories, India. Ethanol (absolute) (MW 46.07) was imported from Changshu Hongsheng Fine Chemical Co., Ltd. Gefitinib Tablets IP 250 mg was purchased from SAMARTH Life Sciences Pvt. Ltd. National Centre for Cell Science (NCCS), Pune, provided the NCI–H522 cell line (Human, Lung, Non-small cell lung cancer) for this study. Penicillin/Streptomycin antibiotic, 3-(4,5- dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Ethanol (EtOH), Phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO) cell culture grade (≥99.8%), Dulbecco's modified Eagle's medium (DMEM), Fetal bovine serum (FBS), 98% sodium hydroxide pellets (NaOH), and the trypsin-EDTA solution was brought from HiMedia Laboratories Pvt. Ltd., India. All of the tissue culture flasks and tissue culture plates were supplied by HiMedia Laboratories Pvt. Ltd., Mumbai (India).
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3

Lipid-based Nanoparticle Formulations for Drug Delivery

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Lig from AstraZeneca (London, UK), DDEA from Neon Laboratory Ltd (Mumbai, India), Compritol ATO 888, Precirol ATO 5, Geleol, and Labrafac PG (LPG) propylene glycol dicaprylate/dicaprate from Gattefosse (St-Priest, France), hydroxypropyl cellulose (HPC)-LF from Loba Chemie Pvt ltd (Mumbai, India), ethyl cellulose (EC; viscosity 20 cps and 50% ethoxy content) from Dow Cellulosic (Midland, MI, USA), and Noveon® AA-1 polycarbophil (PC) from Lubrizol were generous gift samples. Soya lecithin was purchased from Sigma-Aldrich (St Louis, MO, USA), and palmitic acid (PA), stearic acid, Pluronic F 68, and dichloromethane (DCM) were purchased from Himedia Laboratories (Mumbai, India). Milli Q water was used for preparation, and all other chemicals and reagents used were of analytical grade.
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