CD spectra were recorded on a JASCO J-1500 CD spectrometer equipped with a JASCO CTU-100 circulating thermostat unit to control the experimental temperature. In all cases, the concentration of compound 1 was 0.05 mM, and 2 mm quartz cuvettes were used.
Ctu 100
Manufactured by Jasco
The CTU-100 is a laboratory instrument designed for conducting controlled temperature experiments. It provides precise temperature control and monitoring capabilities for a variety of applications.
Automatically generated - may contain errors
Lab products found in correlation
V 750 spectrophotometer, by Jasco (2 mentions)
Fp 8300 spectrofluorometer, by Jasco (2 mentions)
Zuv h30m head unit, by Omron (2 mentions)
Pac 743r, by Jasco (2 mentions)
J 1500 cd spectrometer, by Jasco (1 mentions)
V 750 uv vis spectrophotometer, by Jasco (1 mentions)
J 1500 circular dichroism spectrometer, by Jasco (1 mentions)
7 protocols using ctu 100
1
CD Spectra of Compound 1
2
UV-Vis Absorption Spectra of Compound 1
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UV–Vis absorption spectra were recorded using a JASCO V-750 spectrophotometer equipped with a JASCO CTU-100 circulating thermostat unit to control the experimental temperature. All measurements were performed in chloroform at 25 °C. In all cases, the concentration of compound 1 was 0.05 mM, and 2 mm quartz cuvettes were used.
3
Thermal Difference Spectroscopy of Oligonucleotides
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UV samples of investigated oligonucleotides were prepared using a buffer solution: KH2PO4/K2HPO4 (1 mM, pH 7.0), KCl (5 mM). For each oligonucleotide sample, a UV spectrum was recorded above and below its melting temperature (Tm). All experiments were performed on a Jasco V 750 UV/Vis spectrophotometer (Jasco, Tokyo, Japan) using quartz cuvettes with an optical path of 1 cm and at 25 μM strand concentration. Absorbance spectra were recorded in the 220–340 nm range, with a scan speed of 200 nm min−1 and a data interval of 1 nm. The difference between the UV spectra at high (95 °C) and low (20 °C) temperatures was defined as the TDS; this represents the spectral difference between the unfolded and folded forms. The temperature (20 or 95 °C) was kept constant with a thermostatic circulating water bath for cell holders (Jasco CTU-100). The thermal difference spectra were normalized (+1 for the highest positive peak).
4
Circular Dichroism Spectroscopy of Peptides
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Circular dichroism spectroscopy studies were carried out with a Jasco J-1500 circular dichroism (CD) spectrometer, which was calibrated with (+)-camphor-10-sulfonic acid. A 0.1 mm quartz cuvette was utilised for the measurements, which was kept at a constant temperature of 20 °C with the Jasco CTU-100 circulating thermostat unit. The spectra were recorded at soluble peptide concentrations in PBS as reflected in Table 1 at a resolution of 1 nm.
5
Cloud Point Determination of Triblock Copolymers
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Cloud points (CP) of
the samples were determined with a Jasco V-750 Spectrophotometer operating
at 600 nm with a Jasco CTU-100 circulating thermostat unit attached.
The temperature range was set from 20 to 70 °C at a heating rate
of 1 °C/min. The samples were prepared into 10 mm quartz cuvettes
with a concentration of 0.5 mg/mL and measured during the same day.
Due to inadequate signal in this concentration, PDEGMA417-rnd-POEGMA109-rnd-PAEMA54 and composition 2:1 vol:vol were measured with a concentration
of 1 mg/mL.
the samples were determined with a Jasco V-750 Spectrophotometer operating
at 600 nm with a Jasco CTU-100 circulating thermostat unit attached.
The temperature range was set from 20 to 70 °C at a heating rate
of 1 °C/min. The samples were prepared into 10 mm quartz cuvettes
with a concentration of 0.5 mg/mL and measured during the same day.
Due to inadequate signal in this concentration, PDEGMA417-rnd-POEGMA109-rnd-PAEMA54 and composition 2:1 vol:vol were measured with a concentration
of 1 mg/mL.
6
Quantum Dot-Hemin Complex Fluorescence Assay
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Fluorescence measurements were obtained using 3 mm path length JASCO FMH-857 quartz microcells on a JASCO FP-8300 spectrofluorometer equipped with a JASCO PAC-743R thermocontrolled cell changer and a JASCO CTU-100. Emission at 587 nm was recorded every 10 s with an excitation wavelength of 480 nm. Temperature was kept constant at 30°C. Samples containing QD-hemin complex were prepared as described previously (final AAP-ODN phosphate concentration of 1.85 mM; final QD concentration of 0.1 μM; final hemin concentration of 0.4 μM; final KCl concentration of 400 mM; final buffer concentration of 5 mM). The sample was then subjected to UV (365 nm) irradiation with ZUV-C30H system equipped with a ZUV-H30M head unit (Omron) for 5 min before addition of H2O2 (25 μM) and Amplex Red (25 μM). After equilibration at 30°C for 10 min, fluorescence was measured for 600 s. After measurement, the sample was again irradiated with UV light for 5 min to completely react the unreacted Amplex Red (Zhao et al., 2012 (link)). The same process was then repeated for green light irradiation (520 nm, 1 min, HM-3 monochromatic light source (JASCO)) and subsequent cycles. Minimum intensities were set to zero before average values and SD were obtained.
7
Photoswitchable Fluorescence Measurements
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Aldehyde 1 and amine 2 were synthesized following the procedure reported by Meguellati and co-workers (Meguellati et al., 2013 (link)), and stock solutions of aldehyde 1 in H2O and amine 2 in DMSO were prepared. Fluorescence measurements were obtained using 3 mm path length JASCO FMH-857 quartz microcells on a JASCO FP-8300 spectrofluorometer equipped with a JASCO PAC-743R thermocontrolled cell changer and a JASCO CTU-100. Emission at 520 nm was recorded every 5 min with an excitation wavelength of 480 nm. Temperature was kept constant at 30°C. For subsequent measurements, samples were irradiated with UV (365 nm) light (ZUV-C30H system equipped with a ZUV-H30M head unit (Omron)) for 5 min or UV light for 5 min then green (520 nm) light for 2 min. Due to its degradation at 365 nm irradiation, aldehyde 1 was refreshed after each irradiation cycle. Minimum emission intensities were set to zero before average values and SD were obtained.
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