Blood samples were collected in EDTA tubes and centrifuged at 5000 rpm for 10 min at 4 °C and plasma was kept at −20 °C until further analysis. Blood glucose was monitored by the Accu-Check® Avia monitoring system (Roche Diagnostics, Rotkreuz, Switzerland). Plasma insulin was analysed according to the manufacturer’s instructions (Mercodia Rat Insulin ELISA, Mercodia AB, Uppsala, Sweden). HbA1c was measured from the tail vein and analysed using the DCA Vantage Analyzer (Siemens, Erlangen, Germany).
Accu check avia monitoring system
Manufactured by Roche
Sourced in Switzerland
The Accu-Check® Avia monitoring system is a compact and portable device designed for blood glucose monitoring. It provides users with accurate and reliable measurements of blood glucose levels.
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Lab products found in correlation
4 protocols using accu check avia monitoring system
1
Glycemic Biomarkers in Plasma
2
Glucose Metabolism and Gastric Emptying
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Blood samples were collected in EDTA tubes and centrifuged at 5000 rpm for 10 min at 4 °C, and plasma was kept at −20 °C until further analysis. Blood glucose was monitored by an Accu-Check® Avia monitoring system (Roche Diagnostics, Rotkreuz, Switzerland). HbA1c levels were measured by a DCA Vantage Analyzer (Siemens, Erlangen, Germany). Plasma levels of insulin (Mercodia Rat Insulin ELISA, Mercodia AB, Uppsala, Sweden) were analyzed according to the manufacturers’ instructions. The gastric emptying rate was determined by measuring the acetaminophen in the plasma (Acetaminophen Forensic ELISA kit, Neogen Toxicology, KY, USA).
3
Oral Glucose Tolerance Test in Fasted Rats
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Oral glucose tolerance tests (OGTT) was performed in overnight fasted rats. A glucose bolus (2 g/kg) (Sigma-Aldrich, Copenhagen, Denmark) was administrated p.o. gavage (4 mL/kg) at time 0. Blood samples (EDTA tubes) were collected from the tail vein before glucose administration (0 min) and then the following 15, 30, 60, and 120 minutes post glucose challenge. Blood glucose was monitored 0, 15, 30, 60, 120, and 180 min post-glucose challenge. Blood glucose was monitored by Accu-Check Avia monitoring system (Roche Diagnostics, Rotkreuz, Switzerland).
4
Oral Glucose Tolerance in Mice
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Overnight fasted mice received glucose by oral gavage (5g/kg in C57BL/6JOlaHsd, and 2g/kg in MSNASH/PcoJ, Blood glucose was measured by the Accu-Check® Avia monitoring system (Roche Diagnostics, Rotkreuz, Switzerland) at 0, 15, 30, 60, 120, and 180 mins using a drop of tail blood.
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