Liver tissues were homogenized on ice for 5 sec and then lysed in RIPA buffer containing 2 mM/ml Na3VO4 and 1x protease inhibitor cocktail (Sigma- Aldrich) for 20 min at 4°C. Whole tissue lysates were separated using SDS-PAGE and transferred onto a PVDF membranes. Primary antibodies used were Alpha-catenin (610194; BD Biosciences, San Jose, CA, USA), GAPDH (ab9485; Abcam), Yap (4912; Cell Signaling, Danvers, MA, USA) and phosphor-Yap-Ser127 (4911; Cell Signaling).
Alpha catenin
Manufactured by BD
Sourced in United States
Alpha-catenin is a cytoplasmic protein that plays a key role in the maintenance of cell-cell adhesion. It functions as a linker between the cadherin-catenin complex and the actin cytoskeleton, contributing to the stability of adherens junctions.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700, by Zeiss (2 mentions)
Phosphor yap ser127, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Gapdh, by Abcam (1 mentions)
Stat3 and phospho stat3, by Cell Signaling Technology (1 mentions)
Ha and gfp, by Santa Cruz Biotechnology (1 mentions)
β actin, by Merck Group (1 mentions)
N cadherin, by BD (1 mentions)
Fibronectin, by BD (1 mentions)
E cadherin, by BD (1 mentions)
Vimentin, by BD (1 mentions)
2 protocols using alpha catenin
1
Protein Expression Analysis in Liver Tissue
2
Standardized Immunoblotting and Imaging Protocols
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All analyses were performed as described previously [10 (link), 52 (link)]. For immunoblotting analysis, whole-cell lysates were made using a RIPA lysis buffer, resolved on Bis-Tris 10% gel, and transferred to PVDF membranes. The blots were then probed with various primary antibodies. E-cadherin, fibronectin, N-cadherin, alpha-catenin, and vimentin (BD Biosciences); Twist-1 and DJ-1 (Abcam); STAT3 and phospho-STAT3 (Cell Signaling Technology); HA and GFP (Santa Cruz Biotechnology); V5 (Life Technologies); β-actin (Sigma-Aldrich).
For Immunofluorescence staining, 1 × 104 cells were incubated with specific primary antibodies, washed, and then incubated with secondary antibodies (Invitrogen). Images were acquired using a confocal microscope LSM 700 (ZEISS).
For immunohistochemistry, a tissue microarray slide (CSA) was purchased from Super Bio Chips, and the expression of TrkC at the tissue microarray slide was evaluated by using an indicated antibody. In addition, the evaluation of immunostaining intensity was performed and obtained the score by ImageJ software.
For Immunofluorescence staining, 1 × 104 cells were incubated with specific primary antibodies, washed, and then incubated with secondary antibodies (Invitrogen). Images were acquired using a confocal microscope LSM 700 (ZEISS).
For immunohistochemistry, a tissue microarray slide (CSA) was purchased from Super Bio Chips, and the expression of TrkC at the tissue microarray slide was evaluated by using an indicated antibody. In addition, the evaluation of immunostaining intensity was performed and obtained the score by ImageJ software.
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