Anti cd19 bv650

Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).

For surface staining, cells were incubated with 50 µl purified anti-mouse CD16/32/FcBlock for 10 min at 4 °C, as described previously^{45 (link)}. Additional antibodies were then added in FACS buffer to a final volume of 100 µl at 4 °C for 20 min. After staining, cells were washed twice and resuspended in FACS buffer for analysis. Dead cells were excluded from analysis by fixable viability dye eFluor™ 780 (Thermo Fisher Scientific, MA). Flow cytometry was performed on an LSR Fortessa (BD Biosciences, NJ) with subsequent data analysis using FlowJo software (Tree Star). Cells were quantified by using CountBright Absolute Counting Beads (Thermo Fisher Scientific, MA). The following antibodies were purchased from Biolegend: anti-CD45.2-R-phycoerythrin-cyanine 7 (PECy7) (clone: 104), anti-MHCII I-A/I-E-AF700 (clone: M5/114.15.2), anti-CD11c-BV786 (clone: N418), anti–CD3e-PECy5 (clone: 145-2C11), anti-CD19-BV650 (clone: 6D5), anti-Ly6G-Peridinin-chlorophyll (PerCP)-Cy5.5 (clone: 1A8), anti-F4/80-AF647 (clone: BM8), anti-CD24-BUV395 (clone: M1/69), anti-CD64-PE (clone: X54-5/7.1), anti-Ly6C-BV605 (clone: HK1.5). The following antibodies were purchased from BD Biosciences: anti-CD11b-Brilliant UltraViolet (BUV) 737 (clone: M1/70).