The largest database of trusted experimental protocols

2 3 o 4 benzoyl benzoyl adenosine 5 triphosphate triethylammonium salt bzatp

Manufactured by Merck Group
Sourced in Italy

2′‐(3′)‐O‐(4‐benzoyl‐benzoyl) adenosine 5′‐triphosphate triethylammonium salt (BzATP) is a synthetic compound used in laboratory research. It functions as an agonist for the P2X7 receptor.

Automatically generated - may contain errors

3 protocols using 2 3 o 4 benzoyl benzoyl adenosine 5 triphosphate triethylammonium salt bzatp

1

Synthesis of BzATP Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols
2′‐(3′)‐O‐(4‐benzoyl‐benzoyl) adenosine 5′‐triphosphate triethylammonium salt (BzATP) and all other reagents, unless otherwise stated, were obtained from Sigma Aldrich (Milan, Italy).
+ Open protocol
+ Expand
2

Preparation of Cadmium, Antioxidant, and Ion Stock Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
A stock solution of cadmium chloride ([Cd], 99.99% purity; Sigma-Aldrich, St. Louis, MO) was dissolved in reagent-grade (Picopure®) water at ten parts per thousand and stored at −20°C in 1.5 mL polypropylene tubes. Substocks were made at 10 – 60 ppm (1000x) and stored at room temperature in 1.5 mL polypropylene tubes. N-Acetyl Cysteine ([NAC], >99% purity; Sigma-Aldrich, St. Louis, MO) was dissolved in reagent-grade water at 25 mg/mL and stored at −20°C in 1.5 mL polypropylene tubes. A calcium chloride stock was prepared at 0.5 M and stored at room temperature ([CaCl2], 99% purity; Sigma-Aldrich, St. Louis, MO). A zinc chloride stock was prepared at 0.5 mM and stored at room temperature ([ZnCl2], >98% purity; Sigma-Aldrich, St. Louis, MO). Stock solutions of 2-aminoethyl diphenylborinate ([2-APB], 97% purity; Sigma-Aldrich, St. Louis, MO), NF023 hydrate ([NF023], 98% purity; Sigma-Aldrich, St. Louis, MO), and 2′ (3′)-O- (4-benzoylbenzoyl)adenosine 5′-triphosphate triethylammonium salt ([Bz-ATP], 93% purity; Sigma-Aldrich, St. Louis, MO) were prepared at a concentration of 10 mM and stored at −20°C in amber glass.
+ Open protocol
+ Expand
3

Preparation and Storage of Cadmium and Reagent Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
A stock solution of cadmium chloride ([Cd], 99.99% purity; Sigma-Aldrich, St. Louis, MO) was dissolved in reagent-grade (Picopure ® ) water at ten parts per thousand and stored at -20°C in 1.5 mL polypropylene tubes. Substocks were made at 10 -60 ppm (1000x) and stored at room temperature in 1.5 mL polypropylene tubes. N-Acetyl Cysteine ([NAC], >99% purity; Sigma-Aldrich, St. Louis, MO) was dissolved in reagent-grade water at 25 mg/mL and stored at -20°C in 1.5 mL polypropylene tubes. A calcium chloride stock was prepared at 0.5 M and stored at room temperature ([CaCl 2 ], 99% purity; Sigma-Aldrich, St. Louis, MO). A zinc chloride stock was prepared at 0.5 mM and stored at room temperature ([ZnCl 2 ], >98% purity; Sigma-Aldrich, St. Louis, MO). Stock solutions of 2-aminoethyl diphenylborinate ([2-APB], 97% purity; Sigma-Aldrich, St. Louis, MO), NF023 hydrate ([NF023], 98% purity; Sigma-Aldrich, St. Louis, MO), and 2′ (3′)-O-(4-benzoylbenzoyl)adenosine 5′triphosphate triethylammonium salt ([Bz-ATP], 93% purity; Sigma-Aldrich, St. Louis, MO) were prepared at a concentration of 10 mM and stored at -20°C in amber glass.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!