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The Armadillo GAL4 is a genetic tool used in Drosophila research. It serves as a driver that can be used to express target genes in specific patterns during Drosophila development.

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3 protocols using armadillo gal4

1

Genetic Tools for Drosophila Studies

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armadillo GAL4 (w[*]; P{w[+mW.hs]=GAL4-arm.S}4a P{w[+mW.hs]=GAL4-arm.S}4b; Bloomington Stock Center 1561 that has lost the balancer), eyGAL4, GMRGAL4 (generous gift from Dr. Bob Johnston at Johns Hopkins University), UAS-GFP.nls (P{UAS-GFP.nls}14; Bloomington Stock 4775), UAS-GFP-CAP-D361 (link), tubPGAL4 (Bloomington Stock 5138), repoGAL4 (generous gift from Dr. Heather Broihier at Case Western Reserve University), elavGAL4 (Bloomington Stock Center 8765), c253GAL4 (w[1118]; P{w[+mW.hs]=GawB}C253; Bloomington Stock Center 6980), dpnGAL4 (Bloomington Stock Center 47456), optixGAL4 (w[1118]; P{y[+t7.7] w[+mC]=GMR30D11-GAL4}attP2; Bloomington Stock Center 48098), UAS-gypsyCLEVR and UAS-gypsy-CLEVRΔPBS51 (link).
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2

Ubiquitous SCRTT Expression Using GAL4-UAS

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To ectopically express SCRTT using the GAL4-UAS system [55 (link)], adult virgin female flies of the genotype, y w; P{UAS-ScrTT, w+} were crossed with the GAL4 driver males of the genotype, y w; P{Armadillo-Gal4, w+} (Bloomington stock # 1560) and the progeny expressed SCRTT ubiquitously. To induce expression of SCRTT from the heat-shock promoter, D. melanogaster embryos at 0–16 hours AEL were collected from the apple juice plates on nylon mesh screens with an opening size of 0.3mm stretched across the bottom of a cylindrical basket 3cm in diameter and 5cm high. The embryos were heat-shocked for 30 minutes at 37.5°C by immersion of the filter basket in a circulating water bath.
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3

Ectopic Expression of SCR in Drosophila

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For ectopic expression of SCR, virgin female flies of the genotype, y w; P{UAS-Scr, w+} (Bloomington stock # 7302) were crossed with the GAL4 driver males of the genotype, y w; P{Armadillo-Gal4, w+} (Bloomington stock # 1560) and progeny were collected [55 (link)]. For ectopic expression of SCRTT protein from the heat-shock promoter ScrTT fusion gene, a heat-shock was administered at 5 hours AEL at 36.5°C for 30 minutes. First instar cuticles were prepared as described [56 ] and imaged with darkfield optics on a Leica® Leitz DMRBE microscope.
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