Oasis hlb 3cc 60mg extraction cartridges

Manufactured by Waters Corporation

Sourced in United States

The Oasis HLB (3cc/60mg) extraction cartridges are a type of solid-phase extraction (SPE) device designed for sample preparation. They are used to extract and purify analytes from various liquid samples prior to analysis. The core function of these cartridges is to facilitate the separation and concentration of target compounds from complex matrices.

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Lab products found in correlation

Sep pak c18, by Waters Corporation (2 mentions) 2h4 pgi m, by Cayman Chemical (2 mentions) 2h4 11dtxb2, by Cayman Chemical (2 mentions) 20 20 20 2h3 lte4, by Enzo Life Sciences (2 mentions) Glacial acetic acid, by Merck Group (1 mentions) Ammonium acetate ms grade, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Milli q plus system, by Merck Group (1 mentions) Triple tof 5600 system, by AB Sciex (1 mentions) Sep pakvac 1 cc 100 mg silica cartridges, by Waters Corporation (1 mentions)

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Oasis HLB cartridges (276 citations) Oasis HLB (164 citations) HLB cartridges (38 citations) Oasis HLB extraction cartridges (32 citations) Oasis cartridges (17 citations) Oasis HLB 3cc cartridge (4 citations) Oasis HLB cartridges (3cc 60mg) (2 citations) Oasis HLB cartridges 3cc (2 citations) 3cc HLB cartridge (2 citations)

7 protocols using oasis hlb 3cc 60mg extraction cartridges

Urinary Eicosanoid Metabolites Measurement

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Urine samples from patients were collected at baseline, 2 months, and 6 months of treatment. Urine was stored at −80°C within 1 h of sample collection.⁶⁵ (link)^,⁶⁶ (link) PGE-M and PGD-M are unstable if urine is not stored at −80°C within 90 min of collection. Concentrations of the major urinary prostaglandin (PG)E₂ metabolite, PGE-M; the tetranor-PGE₁, TN-E, the major urinary metabolite of PGD₂, PGD-M; 11-dehydro-thromboxane-B₂, dTxB₂; the metabolite of PGI₂, PGI-M; and leukotriene (LT)E₄ were measured in urine at each time point in all study participant. The assays were performed at the Eicosanoid Core Laboratory at Vanderbilt University Medical Center, in the USA. Samples were shipped on dry ice and stored at −80°C until analysis.
[²H₆]-PGE-M and [²H₁₁]-TN-E were synthesized as previously described.³² (link) [²H₄]-PGI-M and [²H₄]-11dTxB₂ were purchased from Cayman Chemicals (Ann Arbor, MI USA), and [20,20,20-²H₃]-LTE₄ from Enzo Life Sciences (Farmingdale, NY USA). Sep-Pak C18 and Oasis HLB (3cc/60mg) extraction cartridges were obtained from Waters Corporation (Milford, MA USA). The organic reagents were of high-performance Liquid Chromatograph (LC) quality and purchased from Sigma Aldrich (St. Louis, MO USA).

Vinhaes C.L., Fukutani E.R., Santana G.C., Arriaga M.B., Barreto-Duarte B., Araújo-Pereira M., Maggitti-Bezerril M., Andrade A.M., Figueiredo M.C., Milne G.L., Rolla V.C., Kristki A.L., Cordeiro-Santos M., Sterling T.R., Andrade B.B, & Queiroz A.T. (2024). An integrative multi-omics approach to characterize interactions between tuberculosis and diabetes mellitus. iScience, 27(3), 109135.

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Microwave-Assisted Urinary Protein Digestion

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The urinary proteins were digested via the filter-aided sample preparation approach combined with the microwave-assisted protein preparation method, according to a previous study (23 (link)). The proteins were reduced with 20 mM DTT, alkylated with 50 mM 2-iodoacetamide on a 10 kDa filter and washed with urea solution (containing 8 M urea and 0.1 M TBS at pH 8.5) and 25 mM NH₄HCO₃. The trypsin-to-protein ratio was 1:50 and the samples were subjected to high-heat microwave oven irradiation for 1 min. Following digestion, the peptide mixtures were desalted with an octadecyl (C18) solid phase extraction column (Oasis HLB 3cc; 60 mg″ Extraction Cartridges; Waters Corporation, Milford, MA) according to the manufacturer's protocol.

Zou L., Wang X., Guo Z., Sun H., Shao C., Yang Y, & Sun W. (2019). Differential urinary proteomics analysis of myocardial infarction using iTRAQ quantification. Molecular Medicine Reports, 19(5), 3972-3988.

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Serum Metabolite Profiling by UPLC-Q-TOF-MS/MS

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The CQF decoction was extracted with an equal volume of methanol, vortexed for 1 min, and centrifuged at 14,000 rpm for 20 min at 4°C. The supernatant was loaded into sample vials for UPLC-Q-TOF-MS/MS analysis. Phosphoric acid (60 μl) was added to 3 ml serum, vortexed for 30 s, and ultrasonicated for 1 min. The resulting solution was applied to pre-activated OASIS HLB 3 cc (60 mg) extraction cartridges (Waters, Milford, MA, United States). The cartridges were washed with 3 ml water and 3 ml methanol. The methanol eluates were collected and lyophilized. The residues were dissolved in 100 μl of methanol and centrifuged at 14,000 rpm for 20 min at 4°C, and the supernatant was loaded into sample vials for UPLC-Q-TOF-MS/MS analysis.

Luo Q., Huang S., Zhao L., Liu J., Ma Q., Wang Y., Dong Y., Li C, & Qiu P. (2022). Chang qing formula ameliorates colitis-associated colorectal cancer via suppressing IL-17/NF-κB/STAT3 pathway in mice as revealed by network pharmacology study. Frontiers in Pharmacology, 13, 893231.

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Urinary Eicosanoid Biomarkers Quantification

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Concentrations of PGE-M, TN-E, PGD-M, 11dTxB₂, PGI-M, and LTE₄ were measured in urine at each time point in all study participants; these assays were performed at the Eicosanoid Core Laboratory at Vanderbilt University Medical Center.
[²H₆]-PGE-M and [²H₁₁]-TN-E were synthesized as described above (23 (link), 24 (link)). [²H₄]-PGI-M and [²H₄]-11dTxB₂ were purchased from Cayman Chemicals (Ann Arbor, MI USA). [20,20,20-²H₃]-LTE₄ was purchased from Enzo Life Sciences (Farmingdale, NY USA). Sep-Pak C18 and Oasis HLB (3cc/60mg) extraction cartridges were obtained from Waters Corporation (Milford, MA USA). All organic reagents were of high-performance Liquid Chromatograph (LC) quality and purchased from Sigma Aldrich (St. Louis, MO USA).

Arriaga M.B., Karim F., Queiroz A.T., Araújo-Pereira M., Barreto-Duarte B., Sales C., Moosa M.Y., Mazibuko M., Milne G.L., Maruri F., Serezani C.H., Koethe J.R., Figueiredo M.C., Kritski A.L., Cordeiro-Santos M., Rolla V.C., Sterling T.R., Leslie A, & Andrade B.B. (2022). Effect of Dysglycemia on Urinary Lipid Mediator Profiles in Persons With Pulmonary Tuberculosis. Frontiers in Immunology, 13, 919802.

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Quantitative Analysis of Acrylamide and Deoxynivalenol

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Methanol and formic acid (p.a.), both HPLC gradient grade, were obtained from BDH VWR International Ltd. (Poole, UK). Acetonitrile was purchased from J.T. Baker (Deventer, The Netherlands) and ammonium acetate (MS grade) and glacial acetic acid (p.a.) were obtained from Sigma-Aldrich (Vienna, Austria). Standard acrylamide solution was purchased from Sigma-Aldrich (Milan, Italy). Acrylamide internal standard (13C3-acrylamide, 1 mg/mL in methanol) was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). Deionized water was used for all procedures. Water was purified successively by reverse osmosis and a Milli-Q plus system from Millipore (Molsheim, France). Deoxynivalenol standard was obtained from RomerLabs^®Inc. (Tulln, Austria). OASIS^® HLB 3 cc (60 mg) extraction cartridges were purchased from Waters (Manchester, UK). Glass vials with septum screw caps were purchased from Phenomenex (Torrance, CA, USA). Centrifugal filter units (Ultrafree MC 0.22 mm, diameter 10 mm) were obtained from Millipore (Billerica, MA, USA).

Suman M., Generotti S., Cirlini M, & Dall’Asta C. (2019). Acrylamide Reduction Strategy in Combination with Deoxynivalenol Mitigation in Industrial Biscuits Production. Toxins, 11(9), 499.

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Quantitative Analysis of Salicylic Acid in Urine

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The sample preparation for the quantitative analysis of SA was performed following the method described by Schenk et al. [11 ] and included a solid phase extraction. Briefly, 2 μg of the internal standard D4-SA were added to 100 μL of urine and 900 μL of Milli-Q-water. Samples taken during the first 24 h after treatment were diluted 1:10 in Milli-Q-water (12.5 mg/kg and 25 mg/kg), whereas samples of the 50 mg/kg group were diluted 1:50. To enforce hydrolysis of any remaining ASA to SA, the pH was adjusted to 13–14 by addition of 40 μL KOH (1 mol/l). After 20 min an adjustment to pH 7 was achieved by addition of 0.2 mL phosphate buffer (Na₂HPO₄/NaH₂PO₄,0.8 mol/l). After centrifugation (5 min, 500 g), solid phase extraction was performed with conditioned Oasis® HLB 3 cc (60 mg) extraction cartridges (Waters corp., Milford, MA, USA). Samples were then evaporated to dryness in a rotary evaporator and dried residues dissolved in 200 μL ammonium acetate/acetonitrile (30:20, v/v) for HPLC-MS/MS injection.

Buntenkötter K., Osmers M., Schenk I., Schänzer W., Machnik M., Düe M, & Kietzmann M. (2017). Pharmacokinetics and in vitro efficacy of salicylic acid after oral administration of acetylsalicylic acid in horses. BMC Veterinary Research, 13, 28.

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Quantification of 4DO in Rice Roots

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We used 400 pg of deuterium-labeled 5-deoxystrigol (d₆-5DS) as the internal standard.^{23 )} To measure 4DO in rice root exudates, we extracted the hydroponic culture medium with ethyl acetate twice. The organic phase was concentrated in vacuo. To measure 4DO in roots, we homogenized rice roots in ethyl acetate with d₆-5DS added, and the suspension was filtered. The filtrates were dried and dissolved in 10% acetone. The extracts were loaded onto Oasis HLB 3 cc (60 mg) extraction cartridges (Waters, Milford, MA, USA), washed with 10% acetone (6 mL), and eluted with acetone (6 mL). The solutions were concentrated in vacuo and dissolved in 1 mL of ethyl acetate : n-hexane (15 : 85). The SL-containing fractions were loaded onto Sep-Pak Vac 1 cc (100 mg) silica cartridges (Waters), washed with 2 mL of ethyl acetate : n-hexane (15 : 85), and eluted with 3 mL of ethyl acetate : n-hexane (35 : 65). The eluates were concentrated in vacuo.
The dried concentrates were dissolved in deionized water : acetonitrile (1 : 1) and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using a Triple TOF 5600 system (SCIEX), as previously described.^{24 (link))}

Kawada K., Sasaki Y., Asami T., Yajima S, & Ito S. (2022). Insect growth regulators with hydrazide moiety inhibit strigolactone biosynthesis in rice. Journal of Pesticide Science, 47(1), 43-46.

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