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Photon imager

Manufactured by Biospace
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Sourced in France, United States

The Photon Imager is a laboratory instrument designed for the detection and analysis of photons. It is capable of capturing and recording images of photon emission, making it a valuable tool for various scientific applications.

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Lab products found in correlation

M3 vision software, by Biospace (20 mentions) D luciferin, by GoldBio (13 mentions) Photo vision software, by Biospace (5 mentions) D luciferin containing media, by GoldBio (5 mentions) Pearl imager, by LI COR (3 mentions) M3vision analysis software, by Biospace (2 mentions) Luciferin, by Promega (2 mentions) Luciferin, by Interchim (2 mentions) Xylazine, by Bayer (2 mentions) Luciferin, by PerkinElmer (2 mentions) M3 vision, by Biospace (2 mentions) D luciferin, by Promega (2 mentions) D luciferin potassium salt, by Fujifilm (2 mentions) Vivotrack 680 nir fluorescent imaging agent, by PerkinElmer (2 mentions) Luciferin solution, by GoldBio (1 mentions) In vivo jet pei delivery reagent, by Polyplus Transfection (1 mentions) Amersham hyperfilmtm ecl, by GE Healthcare (1 mentions) Perfection 1670 scanner, by Epson (1 mentions) Bwf5 690 8 600 0, by B&W Tek (1 mentions) Balb c nude mice, by Nara Biotech (1 mentions)

79 protocols using photon imager

1

Metastatic Tumor Monitoring in Mice

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Single-cell suspensions of 3×105 B16F10 melanoma cells were injected intravenous into the tail vein of the indicated strains of mice. Mice were sacrificed and lungs were harvested on day 14. Lungs from B16F10 injected mice were fixed in PFA 4% overnight to count B16F10 metastases. E0771-GFP+-Luciferase breast cancer cells were injected into the tail vein of the indicated strains of mice (5×105 cells/mouse). Luciferase expression was then monitored at day 7 and 14 by bioluminescence using PhotonIMAGER (BiospaceLab), following intraperitoneal injection of luciferin (30 mg/kg). After completion of the analysis organ luminescence was assessed. Orthotopic implantation of breast tumors and tumor dissociation was performed as previously described.16 (link) Luciferase expression was monitored by bioluminescence using PhotonIMAGER (BiospaceLab), after intraperitoneal injection of luciferin (30 mg/kg).
Bernard P.L., Delconte R., Pastor S., Laletin V., Costa Da Silva C., Goubard A., Josselin E., Castellano R., Krug A., Vernerey J., Devillier R., Olive D., Verhoeyen E., Vivier E., Huntington N.D., Nunes J, & Guittard G. (2022). Targeting CISH enhances natural cytotoxicity receptor signaling and reduces NK cell exhaustion to improve solid tumor immunity. Journal for Immunotherapy of Cancer, 10(5), e004244.
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2

In vivo Adenoviral Gene Delivery

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Ten-week-old male C57BL/6J mice were anesthetized with isoflurane before the injection through the penis vein with a 150 μl final volume of sterile physiological serum containing TRE-Luc (5 × 108 pfu/mouse) and RSV-β-gal (108 pfu/mouse) adenoviruses. Mice were analyzed 4 days after adenovirus delivery. For in vivo imaging, mice infected with indicated adenoviruses were anesthetized and injected (i.p.) with a dose of 30 mg/kg sterile firefly D-luciferin solution (Biosynth AG, Staad, Switzerland). After 3 min, mice were imaged on the Biospace Photon Imager (Biospace, Nesles-la-Vallée, France), and bioluminescence was analyzed with Biospace software. Luciferase activity was normalized to β-galactosidase activity used as an internal control for adenoviral infection.
Cabrae R., Dubuquoy C., Caüzac M., Morzyglod L., Guilmeau S., Noblet B., Fève B., Postic C., Burnol A.F, & Moldes M. (2020). Insulin activates hepatic Wnt/β-catenin signaling through stearoyl-CoA desaturase 1 and Porcupine. Scientific Reports, 10, 5186.
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3

In vivo Bioluminescence Imaging

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BALB/c or C57BL/6 mice were IV tail-injected with 250 μl of the in vivo-jet PEI delivery reagent (Polyplus transfection, France) complexed with an appropriate luc reporter plasmid. 24 hours later the mice were injected with 250 μl of 3 mg/ml luciferin solution (Gold Biotechnology, USA) and underwent bioluminescence imaging by Biospace Photon Imager (Biospace Lab, France).
Elias A., Spector I., Sogolovsky-Bard I., Gritsenko N., Rask L., Mainbakh Y., Zilberstein Y., Yagil E, & Kolot M. (2016). Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase. Scientific Reports, 6, 24971.
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4

Protein Detection and Imaging Protocol

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Blots from Figs. 1, 3 and Supplementary Figs. 2–4 were obtained by autoradiography using GE Healthcare Amersham HyperfilmTM ECL. Western blots were scanned using Epson Perfection 1670 Scanner. In vivo imaging (Figs. 1a and 2e) was performed using Biospace Photon Imager and analyzed with Biospace software (Biospace, Nesles-la-Vallée, France).
Cabrae R., Dubuquoy C., Caüzac M., Morzyglod L., Guilmeau S., Noblet B., Fève B., Postic C., Burnol A.F, & Moldes M. (2020). Insulin activates hepatic Wnt/β-catenin signaling through stearoyl-CoA desaturase 1 and Porcupine. Scientific Reports, 10, 5186.
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5

Cerenkov Luminescence Imaging of Prostate Cancer Xenografts

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The Cerenkov Luminescence Imaging of the prostate cancer PC3 tumor-bearing mice was carried out at different time points (15 min, 1 and 2 h) after intravenous injection of [90Y]Y-DOTA-Tz. For the in vivo click chemistry studies in the same PC3 xenografts, a bolus of 1 nmol of TCO-PEG4-AR, dissolved in 0.1 mL of saline, was injected in the tail vein, and after 4 h the [90Y]Y-DOTA-Tz (0.1 mL, 7.5 μCi) was injected. Thereafter, the CLI imaging of the PC3 tumor-bearing mice was carried out at different time points (15 min, 1 h). The images were obtained using PhotonIMAGER™ (BioSpace Lab). The optical imaging system was based on intensified CCD camera (25 mm), which had a minimum detectable radiance of 37 photons/s/sr/cm2, minimum image pixel resolution of 2.5 μm and temporal resolution of 23 ms.
D'Onofrio A., Silva F., Gano L., Karczmarczyk U., Mikołajczak R., Garnuszek P, & Paulo A. (2021). Clickable Radiocomplexes With Trivalent Radiometals for Cancer Theranostics: In vitro and in vivo Studies. Frontiers in Medicine, 8, 647379.
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6

Near-infrared Photoimmunotherapy Efficacy

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In vitro, MC38-luc cells were seeded into 12-well plates (2 × 105 cells/well) or a 10 cm dish (2 × 107 cells), incubated for 24 hours, then exposed to CD44-IR700 (10 μg/mL) for 6 hours at 37˚C. Cells were treated with LED or NIR laser light (690 ± 5 nm, BWF5–690-8–600-0.37; B&W TEK INC., Newark, DE, USA) in phenol red-free culture medium. For luciferase activity, cells were exposed to D-luciferin (150 μg/mL; Gold Biotechnology, St. Louis, MO, USA) 1 hour after NIR-PIT treatment, and luciferase activity (photons/min) was obtained on a BLI system (Photon Imager; Biospace Lab, Paris, France) using M3 Vision Software (Biospace Lab). In vivo, D-luciferin (15 mg/mL, 200 μL) was injected intraperitoneally, and the mice were analyzed on a BLI system (Photon Imager) for luciferase activity (photons/min/cm2). ROIs were set to include the entire tumor with the adjacent non-tumor region as background.
Nagaya T., Friedman J., Maruoka Y., Ogata F., Okuyama S., Clavijo P.E., Choyke P.L., Allen C, & Kobayashi H. (2019). Host immunity following near-infrared photoimmunotherapy is enhanced with PD-1 checkpoint blockade to eradicate established antigenic tumors. Cancer immunology research, 7(3), 401-413.
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7

NIR-PIT Efficacy Evaluation in Tumor Model

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Hundred micrograms of APC was administered via the tail vein the day before the experiment. Tumors were irradiated with an NIR-laser (690 ± 5 nm) at a 50 J/cm2 energy density (ML7710, Modulight, Inc. Tampere, Finland). Before and after irradiation, fluorescent images were obtained with the Pearl Imager and LIGHTVISION. For bioluminescence imaging (BLI), D-luciferin (15 mg/mL, 200 μL) (Gold Biotechnology, St. Louis, MO) was injected intraperitoneally, and bioluminescence images were obtained with Photon Imager (Biospace Lab, Nesles la Vallee, France), 15 min after injection. ROIs were placed over the entire tumor. The counts per minute of relative light units were calculated using M3 Vision Software (Biospace Lab) and converted to the percentage decrease in light based on a comparison of post NIR-PIT to pretreatment BLI using the following formula: [(relative light units after treatment)/(relative light units before treatment) × 100 (%)].
Inagaki F.F., Fujimura D., Furusawa A., Okada R., Wakiyama H., Kato T., Choyke P.L, & Kobayashi H. (2021). Fluorescence Imaging of Tumor-Accumulating Antibody-IR700 Conjugates Prior to Near-Infrared Photoimmunotherapy (NIR-PIT) Using a Commercially Available Camera Designed for Indocyanine Green. Molecular pharmaceutics, 18(3), 1238-1246.
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8

Leukemia Engraftment in NOD/SCID Mice

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Female non-obese diabetic/severe combined immunodeficient mice were purchased from Charles River Laboratories Japan. ALL cells (5 × 106 per 100 μl) infected with a lentiviral vector for Luc2 were injected into the tail vein of non-obese diabetic/severe combined immunodeficient mice (age 6–8 weeks). Concentration of L-asp dose in in vivo experiment was decided using the interview form of LEUNASE as reference. The intraperitoneal lethal dose 50 of LEUNASE in mice was estimated to be 10 U/g; therefore, we performed preliminary experiments and ascertained that our mice had a tolerance to repeated intraperitoneal injection of 6 U/g L-asp and 50 mg/kg CQ administration once every day for more than 50 days. Leukemia burden was measured by luciferase activity using a luminometer (Photon Imager, Biospace Lab, Nesles la Vallée, France) after 150 mg/kg D-luciferin (Synchem UG & Co. KG, Felsberg, Germany) injection. All experimental protocols conducted on the mice were approved by the Tokyo Medical and Dental University Animal Care and Use Committee.
Takahashi H., Inoue J., Sakaguchi K., Takagi M., Mizutani S, & Inazawa J. (2017). Autophagy is required for cell survival under L-asparaginase-induced metabolic stress in acute lymphoblastic leukemia cells. Oncogene, 36(30), 4267-4276.
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9

BLI and IR700 Fluorescence Imaging in MC38-luc Tumor Mouse Model

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To obtain BLI images in MC38-luc tumor–bearing mice, D-luciferin (15 mg/mL, 150 mL; Goldbio, St. Louis, MO, USA) was intraperitoneally injected into mice. Luciferase activity was analyzed with a BLI system (Photon Imager; Biospace Lab, Nesles-la-Vallée, France) using relative light units. Regions of interests (ROIs) were placed over the entire tumor. The counts per minute of relative light units were calculated using M3 Vision Software (Biospace Lab, Nesles-la-Vallée, France) and converted to the percentage decrease in light based on a comparison of post NIR-PIT to pre-therapy BLI using the following formula: (relative light units after treatment)/(relative light units before treatment) × 100 (%); [31 (link)]. BLI was performed before and after NIR-PIT (protocol above) on day 0 to day 7. In vivo IR700 fluorescence images were obtained with a Pearl Imager (LI-COR Biosciences, Bad Homburg vor der Höhe, Germany) with a 700-nm fluorescence channel.
Maruoka Y., Furusawa A., Okada R., Inagaki F., Fujimura D., Wakiyama H., Kato T., Nagaya T., Choyke P.L, & Kobayashi H. (2020). Near-Infrared Photoimmunotherapy Combined with CTLA4 Checkpoint Blockade in Syngeneic Mouse Cancer Models. Vaccines, 8(3), 528.
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10

Multimodal Imaging of Orthotopic Prostate Cancer

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We used the Photon Imager (Biospace Lab S.A., France), having the advantages of high-throughput, high-resolution, and multispectral analysis, to colocalize the bioluminescent signal and fluorescence signal of the orthotopic PCa. First, the laser scanned the surface topology (including the surface contour and the overall shape of the structure). Then, the 3D module cooperated with the intensified charge-coupled device for optical signal reception. The Atlas coregistration tool combined 3D module acquisition data with anatomical information from the Digimouse Atlas (University of Southern California) to locate the organ of the optical signal. Volumetric bioluminescent signal and fluorescence signal from the 3D module data were reconstructed using the M3Vision Analysis Software (Biospace Lab).
You H., Shang W., Min X., Weinreb J., Li Q., Leapman M., Wang L, & Tian J. (2020). Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer. Science Advances, 6(6), eaax6040.
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