Ro4929097

Manufactured by MedChemExpress

Sourced in United States

RO4929097 is a small molecule compound that functions as a potent and selective inhibitor of the Notch signaling pathway. It targets the gamma-secretase enzyme, which is responsible for the cleavage and activation of Notch receptors. This compound can be utilized for research purposes to study the role of the Notch pathway in various biological processes and disease models.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Snu398, by American Type Culture Collection (2 mentions) Hamilton syringe, by Hamilton Company (1 mentions) Rhstc1, by R&D Systems (1 mentions) Mhcc 97h, by National Collection of Authenticated Cell Cultures (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Snu 398, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Plc prf 5, by American Type Culture Collection (1 mentions)

3 protocols using ro4929097

Intravitreal Injection of Sox11b Morpholino

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One microliter of 0.25 mM lissamine-tagged control or Sox11b morpholino (MO; Gene Tools, Philomath, OR, USA) was intravitreally injected with a Hamilton syringe (Hamilton Company Inc., Reno, NV, USA) to establish the experimental model. The method of MO electroporation was previously described (Fausett et al., 2008). The following two Sox11b translation-blocking MOs were used in the study: sox11b MO1 (5’-CAT GTT CAA ACA CAC TTT TCC CTC T-3’) (Veldman et al., 2007; Pillai-Kastoori et al., 2014) and sox11b MO2 (5’-TCC GTC TGC TGC ACC ATG TTC-3’).
For Notch inhibition, 1 μL of 250 μM of RO4929097 (MedChemExpress, Monmouth Junction, NJ, USA) was intravitreally injected through the front of the eye from 2 to 4 dpi, twice daily. One microliter of PBS was injected as a negative control.

Song K., Lin Z., Cao L., Lu B., Chen Y., Zhang S., Lu J, & Xu H. (2022). Sox11b regulates the migration and fate determination of Müller glia-derived progenitors during retina regeneration in zebrafish. Neural Regeneration Research, 18(2), 445-450.

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Cell Line Culture and Reagents

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MHCC-97H was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM comprising 10% FBS. SNU-398 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultivated in RPMI 1640 Medium (GIBCO) with 10% FBS. The recombinant human STC1 protein (rhSTC1) and STC1 neutralizing antibody (STC1-Ab) were purchased from R&D Systems. And RO4929097 was purchased from MedChemExpress (MCE).

Bai S., Zhao Y., Chen W., Peng W., Wang Y., Xiong S., A.r.u.n.a., Li Y., Yang Y., Chen S., Cheng B, & Wang R. (2023). The stromal-tumor amplifying STC1-Notch1 feedforward signal promotes the stemness of hepatocellular carcinoma. Journal of Translational Medicine, 21, 236.

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Hepatic Cell Lines Culture Conditions

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Human hepatic L02 cell line, HCC cell lines MHCC-97 h, MHCC-97 L were provided by the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), SNU-398, PLC/PRF/5 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), HLE, Huh-7 were obtained from the Japanese Cancer Research Bank (Tokyo, Japan). SNU-398 were cultured in RPMI 1640 Medium (GIBCO), the rest of the cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; GIBCO, Grand Island, NY, USA). All medium was supplemented with 10% fetal bovine serum (FBS; GIBCO) and 1% penicillin/streptomycin (Invitrogen). Cells were cultured in a humidified atmosphere of 5% CO₂ at 37 °C. The Notch signaling pathway inhibitor RO4929097 was purchased from MedChemExpress (MCE).

Wang X., Wang R., Bai S., Xiong S., Li Y., Liu M., Zhao Z., Wang Y., Zhao Y., Chen W., Billiar T.R, & Cheng B. (2019). Musashi2 contributes to the maintenance of CD44v6+ liver cancer stem cells via notch1 signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 505.

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