Pro caspase 1

Mdivi-1(mitochondrial division inhibitor-1) and TTC (2,3,5-triphenyltetrazolium chloride) were purchased from Sigma Aldrich (St. Louis, Missouri, MO, USA); BSA (bovine serum protein) was obtained from BOSTER (Boster Biological Technology, Wuhan, China, AR1006). Blots were incubated with antibodies against ATF4 (Proteintech, Rosemont, Pennsylvania, USA, 10835-1-AP), Parkin (CST, Boston, Massachusetts, USA, #4211), TOM20 (Proteintech, USA, 11802-1-AP), COX4I1 (Proteintech, USA, 60251-1-Ig), NLRP3 inflammasome (Affinity, Cincinnati, Ohio, USA, DF7438), pro-caspase-1 (ABclonal, Wuhan, China, A0964), cleaved caspase-1 (CST, USA, #67314), pro-IL-1β (ABclonal, China, A11370), cleaved IL-1β (Affinity, USA, AF4006), pro-IL-18 (Proteintech, USA, 10663-1-AP), cleaved IL-18 (R&D Systems, Minneapolis, Minnesota, USA, AF521) and β-actin (ABclonal, China, AC004), followed by the secondary antibodies conjugated to horseradish peroxidase anti-rabbit IgG (H + L) (ABclonal, China, AS014) and anti-mouse IgG (H + L) (ABclonal, China, AS003).
The primary antibodies used for the immunofluorescence analysis were against TOM20 (Proteintech, USA, 11802-1-AP) and COX4I1 proteins (Proteintech, USA, 60251-1-Ig). Brain sections were then incubated with the secondary antibodies goat anti-rabbit DyLight 488 (Abbkine, green, A23240) and goat anti-mouse DyLight 549 (Abbkine, red, A23310).