Filtration of particle solutions was done when necessary using a Millipore Durapore or RC filters (0.22, 0.45 pm). Dialysis was performed against water at room temperature under gentle stirring with regenerated cellulose dialysis tubing (Sigma, mol wt. cut-off > 12000 Da, avg. diameter 33 mm).
Regenerated cellulose dialysis tubing
Manufactured by Merck Group
Regenerated cellulose dialysis tubing is a semi-permeable membrane used for dialysis and separation processes. It allows the passage of small molecules while retaining larger molecules or particles. The tubing is made from regenerated cellulose and is available in various sizes and pore sizes to suit different applications.
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Lab products found in correlation
2 protocols using regenerated cellulose dialysis tubing
1
Particle Solution Filtration and Dialysis
2
Synthesis of Rhodamine-Doped Silica Nanoparticles
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Dye-doped silica NPs, presenting –NH2 functional groups at the surface were prepared following the synthesis sketched in Scheme 4 . 140 mg of Pluronic F127, 60 mg of Pluronic F127-amino and 3.60 mg of the triethoxysilane derivative of Rhodamine B were carefully solubilized with 1 mL of dichloromethane in a 20 mL glass scintillation vial.
The solvent was evaporated from the homogeneous solution by means of a gentle nitrogen flow and subsequently under vacuum at room temperature. One hundred and thirty seven milligram of NaCl were subsequently added, and the mixture was solubilized at 30°C under magnetic stirring with 3.1 mL of CH3COOH 1M. 360 μL of TEOS were added to the solution and reacted for 3 h then 20 μL of TMSCl were added. The mixture was kept under stirring for 20 h at 30°C. The purification was carried out by dialysis vs. MilliQ water, during approximately 48 h, in regenerated cellulose dialysis tubing (Sigma, 12 kDa cut-off) at RT under gentle stirring (Montalti et al., 2014 (link)).
The solvent was evaporated from the homogeneous solution by means of a gentle nitrogen flow and subsequently under vacuum at room temperature. One hundred and thirty seven milligram of NaCl were subsequently added, and the mixture was solubilized at 30°C under magnetic stirring with 3.1 mL of CH3COOH 1M. 360 μL of TEOS were added to the solution and reacted for 3 h then 20 μL of TMSCl were added. The mixture was kept under stirring for 20 h at 30°C. The purification was carried out by dialysis vs. MilliQ water, during approximately 48 h, in regenerated cellulose dialysis tubing (Sigma, 12 kDa cut-off) at RT under gentle stirring (Montalti et al., 2014 (link)).
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