We performed microdialysis in awake and freely moving animals while they were tested in the operant chamber. We used either a concentric microdialysis probe of 2 mm length, 0.5 mm outer diameter, cutoff 30,000 kDa (BASi, Lafayette, IN, United States), or a combination probe (BASi, MD2262) that allowed muscimol infusion in addition to performing microdialysis. The probe was lowered 2 mm below the cannulae guide tip into the ILC. The probe was continuously perfused at a rate of 2 μl/min with artificial cerebrospinal fluid (ACSF) using a microperfusion pump (KDS100L model from KD Scientific, Inc., Holliston, MA, United States). The composition of the ACSF solution was 147 mM NaCl, 1.2 mM CaCl2, 4 mM KCl, 2 mM Na2HPO4, and 0.2 mM NaH2 PO4 (pH 7.4), freshly prepared with sterile distilled water. After a stabilization period of 120 min, three basal samples of 10 min each were collected in separate tubes containing 2.0 μl of 0.2 N perchloric acid, after which samples were collected every 10 min during all experimental protocols.
Kds100l model
Manufactured by KD Scientific
Sourced in United States
The KDS100L is a precision syringe pump that provides accurate and reliable fluid delivery. It features a wide flow rate range, high resolution, and compatibility with a variety of syringe sizes. The core function of the KDS100L is to enable precise and controlled infusion or withdrawal of fluids.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using kds100l model
1
Microdialysis in Awake Animal Operant Tests
2
Microdialysis for Neurotransmitter Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We performed microdialysis to measure the extracellular concentration of these neurotransmitters every 10 min. On day 8 after surgery, a concentric microdialysis probe of 2 mm length, 0.5 mm outer diameter, cut-off 30 000 kDa (BASi) or a combination probe (MD2262; BASi), which allowed substances to be microinjected in addition to performing microdialysis, was lowered 2 mm below the guide cannula tip into the IL. The probe was perfused continuously at a rate of 2 μl/min with ACSF using a microperfusion pump (KDS100L model from KD Scientific Inc., Holliston, Massachusetts, USA). The composition of the ACSF solution was as follows: 147 mmol/l NaCl; 1.2 mmol/l CaCl2; 4 mmol/l KCl; 2 mmol/l Na2HPO4; 0.2 mmol/l NaH2 PO4; and pH 7.4, freshly prepared with sterile-distilled water. After a stabilization period (120 min), three basal samples were collected in 2.0 μl of 0.2 N perchloric acid before the experiments began.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!