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Pan keratin

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Pan-keratin is a protein specific antibody used for the detection of keratin proteins in a variety of cell and tissue types. It recognizes multiple keratin isotypes, providing a broad-spectrum detection of keratins.

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Lab products found in correlation

Lsm 780 confocal microscope, by Zeiss (6 mentions) Vimentin, by Cell Signaling Technology (4 mentions) α sma, by Thermo Fisher Scientific (3 mentions) β catenin, by Cell Signaling Technology (2 mentions) N cadherin, by Santa Cruz Biotechnology (2 mentions) Nedd9, by Abcam (2 mentions) P130cas, by Santa Cruz Biotechnology (2 mentions) Psrcy527, by Cell Signaling Technology (2 mentions) Sv40 t ag, by Santa Cruz Biotechnology (2 mentions) P stat3 y705, by Cell Signaling Technology (2 mentions) Stat3, by Cell Signaling Technology (2 mentions) E cadherin, by Santa Cruz Biotechnology (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Cd107b, by BD (1 mentions) F4 80, by Novus Biologicals (1 mentions) F4 80, by Cell Signaling Technology (1 mentions) Cd163, by Biorbyt (1 mentions) Tom20, by Santa Cruz Biotechnology (1 mentions) Yap taz, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-pan-keratin (5 citations) Pan-Keratin C11 (4 citations) Pan-keratin antibody (3 citations)

18 protocols using pan keratin

1

Immunofluorescence Staining of Tumor Tissues

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Immunofluorescence (IF) staining was performed on paraffin-embedded sections fixed in 4% buffered formalin. Tumor tissue was deparaffinized, hydrated, and antigen epitope retrieval was performed. Sections were incubated with primary antibodies overnight at 4°C. The following antibodies were used: CD107b (1:100, cat#550292, BD Pharmingen), CD163 (1:100, cat#orb13303, Biorbyt), Gpr18 (1:100, cat#NBP2–24918SS, Novus Biologicals), F4/80 (1:50, cat#NB600–404SS, Novus Biologicals), Foxp3 (1:1000, cat# ab20034, Abcam), F4/80 (1:100, cat# 70076, Cell Signaling), and Pan-Keratin (1:100, cat# 4545, Cell Signaling). Primary antibody incubation was followed by treatment with AlexaFlour-tagged IgG secondary antibody (1:400 dilution, Life Technologies). Nuclei were counterstained with 6-diamidino-2-phenylindole dihydrochloride hydrate (DAPI). Images were captured with a 20x objective using a Nikon fluorescence or Olympus confocal microscope. Analysis was performed on 6–8 random fields for each of the experimental and control groups.
Bhatia S., Oweida A., Lennon S., Darragh L.B., Milner D., Phan A.V., Mueller A.C., Van Court B., Raben D., Serkova N.J., Wang X.J., Jimeno A., Clambey E.T., Pasquale E.B, & Karam S.D. (2019). Inhibition of EphB4-ephrin-B2 signaling reprograms the tumor immune microenvironment in head and neck cancers. Cancer research, 79(10), 2722-2735.
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2

Immunofluorescence Labeling of Cellular Markers

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The following primary antibodies were used in this study: TOM20 (Santa Cruz Biotechnology, sc-17764, 1:50), YAP/TAZ (Cell Signaling, rabbit mAb 8418, 1:200), pan-keratin (Cell Signaling, C11 mouse mAb 4545, 1:500), and vimentin (Cell Signaling, rabbit mAb 5741, 1:200). Alexa Fluor-conjugated secondary antibodies (Life Technologies) were used at a dilution of 1:500.
Begum H.M., Ta H.P., Zhou H., Ando Y., Kang D., Nemes K., Mariano C.F., Hao J., Yu M, & Shen K. (2019). Spatial Regulation of Mitochondrial Heterogeneity by Stromal Confinement in Micropatterned Tumor Models. Scientific Reports, 9, 11187.
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3

Protein Extraction and Western Blot Analysis

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The concentrations of protein extractions were determined using a BCA Protein Assay kit (Takara, Dalian, China). Here, 10 μg protein extractions were loaded onto each lane of a denaturing 4%–20% gradient gel and fractionated. Proteins were transferred to the Hydrophobic Polyvinylidene Fluoride (PVDF) membrane, and the blot was probed with an N-cadherin (610920, BD Pharmingen, San Diego, CA, USA), E-cadherin (610181, BD Pharmingen, San Diego, CA, USA), vimentin (5741S, Cell signaling, Danvers, MA, USA), GAPDH (60004-1-Ig, Proteintech, Wuhan, China), plectin (ab32528, Abcam, Cambridge, England), CDC42 (2462S, Cell signaling, Danvers, MA, USA), pan-keratin (4545S, Cell signaling, Danvers, MA, USA), β-catenin (610153, BD Pharmingen, USA), β-Actin (A00702-100, Genscript, Nanjing, China) and antibody, ATXN2 (21776-1-AP, Proteintech, Wuhan, China). Western blots were imaged and quantitated with a Bio-Rad ChemiDoc XRS+ System.
Lu Y., Dai J., Kong N., Liu J., Gong J, & Yao Y. (2019). Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator. Cells, 8(8), 931.
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4

Vitamin D Regulation of Epithelial Cells

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Vitamin D3 and 1,25(OH)2D3 were purchased from Shanghai General Pharmaceutical Company (Shanghai, People’s Republic of China) and Sigma-Aldrich Co. (St Louis, MO, USA), respectively. Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12) medium and supplementaries were purchased from Thermo Fischer Scientific, (Waltham, MA, USA). The antibodies to pan-keratin, E-adhesion, and β-catenin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), VDR from Novus Biologicals (Littleton, CO, USA), and second antibody from Thermo Fischer Scientific, C57BL/6 female mice were obtained from the Experimental Animal Center (Soochow University, Suzhou, People’s Republic of China). Care and treatment of mice were performed in strict accordance with the Guide for the Care and Use of Laboratory Animals of the National Science and Technology Committee. All protocols were approved by the Medical Research Ethics Committee of Jiangsu Province, People’s Republic of China.
Liu L., Hu Z., Zhang H., Hou Y., Zhang Z., Zhou G, & Li B. (2016). Vitamin D postpones the progression of epithelial ovarian cancer induced by 7, 12-dimethylbenz [a] anthracene both in vitro and in vivo. OncoTargets and therapy, 9, 2365-2375.
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5

Protein Expression Analysis in Tissue Lysates

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Tissue was lysed in radio-immuno-precipitation assay buffer (RIPA) (Boston BioProducts, Ashland, MA), 40ug were fractionated by sodium dodecyl sulfide polyacrylamide gel electrophoresis (SDS-PAGE) using 3%−8% Tris-Acetate gels (NuPage Novex Mini Gel), and the protein was transferred to polyvinylidene diflouride membranes (PVDF) (Millipore, Billerica, MA) and incubated overnight at 4 degrees C with primary antibodies against Transforming growth factor β (TGF-β), Janus family of tyrosine kinase-2 (Jak2), STAT 3, matrix metallopeptidase-9 (MMP-9), SMAD2/3, monocyte chemoattractant protein-1 (MCP-1), α-tubulin, N- Cadherin, α-fodrin, desmin, connexin 43, pan keratin, ß-tubulin, Troponin I, vimentin, filamin, ß-Actin and troponin T (Cell Signaling, Danvers, MA). The following day, membranes were incubated with the appropriate horseradish peroxidase (HRP)-linked secondary antibody for 1h at room temperature (Jackson ImmunoResearch, West Grove, PA). Immune complexes were visualized with enhanced chemi luminescence, images were captured with a digital camera system (G-Box, Syngene, Cambridge, England), and band densitometry was quantified as arbitrary light units using Image J Software (National Institutes of Health, Bethesda, MD). Anti-GAPDH antibody (Cell Signaling) was used on all membranes to correct for loading error. Representative images have been included in the manuscript.
Potz B.A., Sabe A.A., Sabe S.A., Lawandy I.J., Abid M.R., Clements R.T, & Sellke F.W. (2020). Calpain Inhibition Decreases Myocardial Fibrosis in Chronically Ischemic Hypercholesterolemic Swine. The Journal of thoracic and cardiovascular surgery, 163(1), e11-e27.
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6

Signaling Pathways Activation Analysis

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Neutral red dye, SB203580 (p38 MAPK inhibitor) and β-actin antibody (A5441) were purchased from Sigma-Aldrich (Oakville, Ontario, Canada). The protein inhibitor cocktail was purchased from Roche (Mississauga, Ontario, Canada). Antibodies against pan-keratin (#4545), p38 (#8690), phospho-p38 (#4511), cleaved PARP (#5625), PARP (#9542), p53 (#2527), phospho-p53 (Ser15) (#9284), phospho-p53 (Ser46) (#2521) phospho-p53 (Ser392) (#9281), survivin (#2808), phospho-MAPKAPK2 (#3007), MAPKAPK2 (#12155), phospho-Chk2 (#2179), Chk2 (#6334), and Alexa Fluor® goat anti-rabbit secondary antibody (#8889) were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody against PAX8 (10336-1-AP) was purchased from Proteintech (Rosemont, IL, USA).
Han X., Chen H., Zhou J., Steed H., Postovit L.M, & Fu Y. (2018). Pharmacological Inhibition of p38 MAPK by SB203580 Increases Resistance to Carboplatin in A2780cp Cells and Promotes Growth in Primary Ovarian Cancer Cells. International Journal of Molecular Sciences, 19(8), 2184.
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7

Cisplatin-Induced EMT Signaling Pathway

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Cisplatin (P4394) was purchased from Sigma (USA). BCA protein assay kit (23225) was purchased from Thermo-Scientific (USA). Nitrocellulose membranes (1060003) were purchased from GE (USA). E-cadherin (#3195), Snail (#3879), Slug (#9585), Vimentin (#5741), p-ERK (#9106), ERK (#4695), and pan-keratin (#4545) antibodies were purchased from Cell signaling technology (USA). β-Actin (Sc-47778) antibody was purchased from Santa Cruz (USA). Ki-67 antibody (ab15580) was purchased from Abcam (USA). IR800 dye-conjugated rabbit (#926-32213) and mouse (#926-32212) secondary antibodies were purchased from Li-COR Biosciences (USA). Secondary antibody labeled with Cy3 (#711-165-152) was purchased from Jackson ImmunoResearch (USA) and Biotin-rabbit IgG (656140) from Invitrogen, and streptavidin-HRP (P0397) from DAKO (USA).
Lee C., Ryu H.W., Kim S., Kim M., Oh S.R., Ahn K.S, & Park J. (2020). Verminoside from Pseudolysimachion rotundum var. subintegrum sensitizes cisplatin-resistant cancer cells and suppresses metastatic growth of human breast cancer. Scientific Reports, 10, 20337.
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8

Comprehensive Cellular Protein Analysis

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Focal Adhesion Protein Antibody Kit (Cat. No. 13430; Cell Signaling Technology, Danvers, MA) includes: FAK, paxillin, talin‐1, tensin 2, vinculin; Cytoskeletal Marker Antibody Kit (Cat. No. 8614; Cell Signaling Technology, Danvers, MA) includes: keratin 17, pan‐keratin, β‐tubulin, vimentin, β‐Actin; and GAPDH (Cat. No. 8884; Cell Signaling Technology, Danvers, MA).
Halim A., Narayanan G., Hato T., Ho L., Wan D., Siedlecki A.M., Rhee E.P., Allegretti A.S., Nigwekar S.U., Zehnder D., Hiemstra T.F., Bonventre J.V., Charytan D.M., Kalim S., Thadhani R., Lu T, & Lim K. (2022). Myocardial Cytoskeletal Adaptations in Advanced Kidney Disease. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(5), e022991.
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9

Immunohistochemical Analysis of Mouse Tumor and Liver Tissue

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Tumor and adjacent liver tissue from mice were fixed in 4% formalin for 48 hours, embedded in paraffin, and sectioned into 3- to 5-μm slices. Formalin-fixed, paraffin-embedded mouse tumor and adjacent liver sections were deparaffinized, hydrated, and incubated with primary antibody overnight at 4°C. Sections were stained with the following primary antibodies: keratin 19 (KRT19) (#12434; Cell Signaling), pan-keratin (#4545S; Cell Signaling), hepatocyte nuclear factor 4α (#ab181604; Abcam), CD8a (#MA1-70041; Thermo Fisher), CD11a (#48-0111-82; eBioscience), and Ki67 APC-labeled (#652406; BioLegend). For immunohistochemistry, the VECTASTAIN Elite ABC-HRP Kit, peroxidase (mouse/rabbit/rat IgG) (#PK-6102/6101/6104; Vector Laboratories), and ImmPACT DAB Substrate Kit, peroxidase (HRP) (#SK-4105; Vector Laboratories) were used. Nuclei were counterstained with hematoxylin. Images were captured with a Zeiss AXIO Scope A1 (Zeiss). For immunofluorescence, goat anti-mouse/rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488/568 (#A-11001/A-11011; Thermo Fisher) were used. Nuclei were counterstained with Hoechst. Images were captured with a LSM780 confocal microscope (Zeiss) and analyzed with Fiji.
Loeuillard E.J., Li B., Stumpf H.E., Yang J., Willhite J.R., Tomlinson J.L., Rohakhtar F.R., Simon V.A., Graham R.P., Smoot R.L., Dong H, & Ilyas S.I. (2024). Noncanonical TRAIL Signaling Promotes Myeloid-Derived Suppressor Cell Abundance and Tumor Growth in Cholangiocarcinoma. Cellular and Molecular Gastroenterology and Hepatology, 17(5), 853-876.
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10

Immunofluorescence Staining of Tumor Tissue

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Tissue specimens of 10 μm thickness were cut, mounted on slides, and stored at −80 °C prior to immunofluorescence (IF) staining. The slides were fixed with ice-cold methanol for 10 mins, followed by PBS washing and permeabilizing in 0.2% PBS-triton for 15 mins. After PBS washing three times, the sections were blocked for 30 mins before overnight incubation with the antibodies (α-Sma, ThermoFisher SCIENTIFIC; Cd31, BioLegend; pan-keratin, Cell Signaling Technology, Danvers, MA, USA). A mounting reagent (ProlongTM Glass Antifade Mountant, Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA) was used to cover the slides. The stained images were captured on a Carl Zeiss LSM 780 confocal microscope. In total, three tumors per group were used for IF staining.
Deng M., Dai W., Yu V.Z., Tao L, & Lung M.L. (2020). Cylindromatosis Lysine 63 Deubiquitinase (CYLD) Regulates NF-kB Signaling Pathway and Modulates Fibroblast and Endothelial Cells Recruitment in Nasopharyngeal Carcinoma. Cancers, 12(7), 1924.
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