Condition medium were analyzed for human CCL2 (Peprotech, cat no.900-K31) or murine CCL2 (Peprotech, cat no.900-K126) using commercial ELISA protocols. For CD154 ELISA, high-protein binding plates were coated with 1 μg/ml capture CD154 antibody (BD Bioscience cat no.552559) overnight. Plates were blocked in PBS/10% BSA for 2 hours. Samples were diluted 1:1 in PBS/1% BSA/Tween-20 and plated at 100 μl/well for 24 hours at 4oC. Wells were incubated with 0.5 μg/ml biotinylated anti-CD154 (BD Biosciences, cat no.552560) for 2 hours, followed by streptavidin-peroxidase (Vector Laboratories, cat no.PK-6100) for 30 minutes. Reactions were catalyzed using tetramethylbenzidine substrate (Pierce cat no.34028) and read at OD450nM using a BioTek Microplate Reader.
Biotinylated anti cd154
Manufactured by BD
Biotinylated anti-CD154 is a laboratory reagent used for the detection and analysis of CD154, also known as CD40 ligand (CD40L), in various biological samples. It is a biotin-labeled monoclonal antibody that specifically binds to the CD154 protein. This product is intended for research use only and its specific applications may vary depending on the user's experimental requirements.
Automatically generated - may contain errors
2 protocols using biotinylated anti cd154
1
ELISA Quantification of CCL2 and CD154
2
ELISA Quantification of CCL2 and CD154
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Condition medium were analyzed for human CCL2 (Peprotech, cat no.900-K31) or murine CCL2 (Peprotech, cat no.900-K126) using commercial ELISA protocols. For CD154 ELISA, high-protein binding plates were coated with 1 μg/ml capture CD154 antibody (BD Bioscience cat no.552559) overnight. Plates were blocked in PBS/10% BSA for 2 hours. Samples were diluted 1:1 in PBS/1% BSA/Tween-20 and plated at 100 μl/well for 24 hours at 4oC. Wells were incubated with 0.5 μg/ml biotinylated anti-CD154 (BD Biosciences, cat no.552560) for 2 hours, followed by streptavidin-peroxidase (Vector Laboratories, cat no.PK-6100) for 30 minutes. Reactions were catalyzed using tetramethylbenzidine substrate (Pierce cat no.34028) and read at OD450nM using a BioTek Microplate Reader.
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