Log In Sign up
The largest database of trusted experimental protocols

Pcmv red firefly luc vector

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The PCMV-Red firefly Luc vector is a plasmid designed for the expression of firefly luciferase under the control of a CMV promoter. The vector includes a red fluorescent protein (RFP) reporter gene for visualization of transfection efficiency.

Automatically generated - may contain errors

Lab products found in correlation

Ha ubiquitin, by Addgene (2 mentions) Pcdna3 myr ha akt1, by Addgene (2 mentions) Pcdna3 jnk1a1 apf dn jnk, by Addgene (2 mentions) Pmcs green renilla luc vector, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Lb 940 luminometer, by Berthold Technologies (1 mentions)

3 protocols using pcmv red firefly luc vector

1

Luciferase assay in RPTEC cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RPTEC cells were seeded into 24-well plates at a density of 1.5 x 105 cells/well. Cells were transfected using Lipofectamine 2000 (ThermoFisher scientific, Waltham, Massachusetts, USA), with 600 ng of DNA and 30 ng of a control vector for transfection efficiency, using the constitutive expression pCMV-Red Firefly Luc Vector (16156, ThermoFisher scientific, Waltham, MA, USA). After transfection, cells were exposed to 10 ng TNFα (< 0.1 endotoxin units per μg of protein, 210-TA, R&D Systems, Minneapolis, MN, USA) or the vehicle control for 24 h and then luciferase activity was determined using a Gaussia-firefly luciferase dual assay kit (16181, ThermoFisher scientific, Waltham, MA, USA) and a LB940 luminometer (Berthold, Germany).
Han C., Zheng J., Wang F., Lu Q., Chen Q., Hu A., Visentin M., Kullak-Ublick G.A., Gai Z, & Chu L. (2022). The Role of NF-kB in the Downregulation of Organic Cation Transporter 2 Expression and Renal Cation Secretion in Kidney Disease. Frontiers in Medicine, 8, 800421.
+ Open protocol
+ Expand
2

Plasmid Constructs for Diabetes Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
pCMV-myc-MST1 and kinase-dead (MST1-K59; dnMST1) was kindly provided by Dr. Junichi Sadoshima and Dr. Yasuhiro Maejima (UMDNJ, New Jersey Medical School) 30 (link). Mouse pB.RSV.PDX1-GFP plasmid was kindly provided by Dr. Ingo Leibiger (Karolinska University, Stockholm). pcDNA3 Myr-HA Akt1, HA-Ubiquitin and pCDNA3 Jnk1a1 (apf)(dn-JNK) plasmids were obtained from Addgene (Cambridge, MA). Mouse PDX1 mutants (T11, T126, T152, T155, T214 and T231) in pCGIG5 vector were generated by site-directed mutagenesis as described previously 38 (link). All mutations were verified by sequencing. To make bacterial expression plasmids for PDX1 mutants, the complete mouse PDX1 CDS (wild type and mutants) has been amplified by PCR using a specific set of primers from pCGIG5 plasmids and cloned into a pGEX-6P-1 bacterial expression vector (kindly provided by Dr. Reinhard Walther, University of Greifswald). The rat insulin driven luciferase vector (RIP-Luc) was constructed by subcloning a 700bp fragment containing -660bp of the rat 2 insulin promoter (kindly provided by Dr. Rolf Zinkernagel, University of Zurich) into a pMCS-Green-Renilla-Luc vector (Thermo Scientific). pCMV-Red firefly Luc vector was obtained from Thermo Scientific.
Ardestani A., Paroni F., Azizi Z., Kaur S., Khobragade V., Yuan T., Frogne T., Tao W., Oberholzer J., Pattou F., Conte J.K, & Maedler K. (2014). MST1 is a novel regulator of apoptosis in pancreatic beta-cells. Nature medicine, 20(4), 385-397.
+ Open protocol
+ Expand
3

Plasmid Constructs for Diabetes Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
pCMV-myc-MST1 and kinase-dead (MST1-K59; dnMST1) was kindly provided by Dr. Junichi Sadoshima and Dr. Yasuhiro Maejima (UMDNJ, New Jersey Medical School) 30 (link). Mouse pB.RSV.PDX1-GFP plasmid was kindly provided by Dr. Ingo Leibiger (Karolinska University, Stockholm). pcDNA3 Myr-HA Akt1, HA-Ubiquitin and pCDNA3 Jnk1a1 (apf)(dn-JNK) plasmids were obtained from Addgene (Cambridge, MA). Mouse PDX1 mutants (T11, T126, T152, T155, T214 and T231) in pCGIG5 vector were generated by site-directed mutagenesis as described previously 38 (link). All mutations were verified by sequencing. To make bacterial expression plasmids for PDX1 mutants, the complete mouse PDX1 CDS (wild type and mutants) has been amplified by PCR using a specific set of primers from pCGIG5 plasmids and cloned into a pGEX-6P-1 bacterial expression vector (kindly provided by Dr. Reinhard Walther, University of Greifswald). The rat insulin driven luciferase vector (RIP-Luc) was constructed by subcloning a 700bp fragment containing -660bp of the rat 2 insulin promoter (kindly provided by Dr. Rolf Zinkernagel, University of Zurich) into a pMCS-Green-Renilla-Luc vector (Thermo Scientific). pCMV-Red firefly Luc vector was obtained from Thermo Scientific.
Ardestani A., Paroni F., Azizi Z., Kaur S., Khobragade V., Yuan T., Frogne T., Tao W., Oberholzer J., Pattou F., Conte J.K, & Maedler K. (2014). MST1 is a novel regulator of apoptosis in pancreatic beta-cells. Nature medicine, 20(4), 385-397.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!