Log In Sign up
The largest database of trusted experimental protocols

Macs purified

Manufactured by Miltenyi Biotec
Visit Supplier
Sourced in United States

MACS purified is a versatile tool for the separation and enrichment of target cells from complex cell samples. It utilizes magnetic nanoparticles conjugated to specific antibodies or ligands to selectively label and separate the desired cell population. The core function of MACS purified is to provide a reliable and efficient method for isolating cells of interest from heterogeneous mixtures, enabling downstream applications and research.

Automatically generated - may contain errors

Lab products found in correlation

Aria 2, by BD (1 mentions) Lsr 2, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by R&D Systems (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Polyinosinic polycytidylic acid, by InvivoGen (1 mentions) Cpg a odn1585, by InvivoGen (1 mentions) Recombinant mouse il 4, by R&D Systems (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Hm40 3, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igm f ab 2, by Jackson ImmunoResearch (1 mentions)

5 protocols using macs purified

1

Bone Marrow Cell Isolation and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow cells were harvested from femurs and tibiae. Lin− cells were MACS-purified (Miltenyi) and stained with fluorescent antibodies for cell analysis or sorting. For cell cycle and phospho-Flow analyses by flow cytometry, Lin− cells were fixed, permeablized and stained with the indicated antibodies, and analyzed on a BD LSRII or Aria II. Data were analyzed using FlowJo software (Tree Star). Detailed procedures and reagents are included in supplementary information.
An W., Nadeau S.A., Mohapatra B.C., Feng D., Zutshi N., Storck M.D., Arya P., Talmadge J.E., Meza J.L., Band V, & Band H. (2015). Loss of Cbl and Cbl-b ubiquitin ligases abrogates hematopoietic stem cell quiescence and sensitizes leukemic disease to chemotherapy. Oncotarget, 6(12), 10498-10509.
+ Open protocol
+ Expand
2

Parasite Invasion Susceptibility Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The assay was performed and analyzed as in25 (link) using two different concentrations of CellTrace Far Red DDAO (Invitrogen Life Technologies/Molecular Probes): 1 µM (high) or 0.1 µM (low). Differentially labeled RBCs were combined in equal density in triplicate into a 96 well plate and seeded with MACS purified (Miltenyi Biotec) late stage parasites to achieve 1.5–2% pRBCs in 1% hematocrit. Plates were maintained for 12–18 hours to allow for schizont rupture and subsequent invasion of labeled RBCs. Invasion into high and low DDAO RBCs was detected by DNA dye Sybr Green I, and analyzed by flow cytometry as in1 (link), and the ratio of the prevalences of parasitized high to low DDAO RBCs was calculated to generate the “Susceptibility Index”. The parasite strains used were parent strain 3D7 and knockout strains ΔPfRh1, ΔPfRh2a, and ΔPfRh2b, all assayed in parallel for invasion into the same donor RBCs from non-anemic controls and anemic, pregnant subjects (n = 15). This allowed for comparison of invasion deficits of parent and transgenic parasite strains into anemic RBCs versus non-anemic RBCs.
Goheen M.M., Bah A., Wegmüller R., Verhoef H., Darboe B., Danso E., Prentice A.M, & Cerami C. (2017). Host iron status and erythropoietic response to iron supplementation determines susceptibility to the RBC stage of falciparum malaria during pregnancy. Scientific Reports, 7, 17674.
+ Open protocol
+ Expand
3

Dendritic Cell Activation and T Cell Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
DC (2 × 106 cells/ml) were cultured in complete RPMI 1640 supplemented with 10% FCS alone or in the presence of R837 (0.5 μg/ml), LPS (5 μg/ml), CpGA-ODN1585 (5 μg/ml), or polyinosinic:polycytidylic acid [poly(I:C); 5 μg/ml] (all from InvivoGen, San Diego, CA). Cytokine secretion was determined at 20 h of culture by ELISA. For co-culture experiments, MACS purified (Miltenyi Biotech, San Diego, CA, USA) CD4+ T cells were labeled with CFSE (1 μM; R&D Systems; Minneapolis, MN, USA) for 5 min at RT, followed by extensive washing in complete RPMI 1640, prior to culture. DC were cultured at a ratio of 1:20 with CD4+ T cells (1.25 × 105/ml) in the presence of anti-CD3 (1 μg/ml). Cells were collected on day 3 of culture and stained with CD4 antibody for detection of T cells and analyzed by Flow cytometry using FACSDiva Software on an LSRII (BD Biosciences; San Jose, CA, USA).
Tu H., Burke T.M., Oderup C., Huang K., Wong K., Lewén S., LaJevic M, & Zabel B.A. (2014). Robust Expansion of Dendritic Cells in vivo by Hydrodynamic FLT3L-FC Gene Transfer. Journal of immunological methods, 0, 69-73.
+ Open protocol
+ Expand
4

In Vitro B Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MACS-purified (Miltenyi Biotec) CD43− or CD19+ B cells were activated in vitro at a density of 1–3 × 106 cells/ml with 2 μg/ml of anti-CD40 clone HM40-3 (eBiosciences) plus 25 ng/ml of recombinant mouse IL-4 (R&D Systems), 10 μg/ml of goat F(ab′)2 anti-mouse IgM (Jackson Immunoresearch), LPS (20 μg/ml), or LPS + IL-4 (Sigma).
Ha N., Pham D.H., Shahsafaei A., Naruse C., Asano M, & Thai T.H. (2014). HP-1γ Controls High-Affinity Antibody Response to T-Dependent Antigens. Frontiers in Immunology, 5, 271.
+ Open protocol
+ Expand
5

GUCY2C-Derived Peptide Library Immunoassay

Check if the same lab product or an alternative is used in the 5 most similar protocols
A library of 105 GUCY2CECD-derived peptides 15 amino acids in length, with 11 amino acid overlap with adjacent peptides was synthesized (JPT Peptide Technologies). Aliquots of individual peptides or pools of 10–11 peptides were dissolved in DMSO and used in ELISpot assays at a final concentration of 5–10 μg/mL of each peptide with ≤1% DMSO. Splenocytes were collected from GUCY2C−/− BALB/c mice 10 days after immunization with Ad5-GUCY2C and CD4+ T cells were MACS purified (Miltenyi Biotec) and mixed with naïve splenocytes as APCs and peptides for ELISpot analysis.
Snook A.E., Magee M.S., Schulz S, & Waldman S.A. (2014). Self-tolerance eliminates CD4+ T, but not CD8+ T or B, cells corrupting cancer immunotherapy. European journal of immunology, 44(7), 1956-1966.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!