Diethyldithiocarbamic acid

Manufactured by Merck Group

Sourced in United States

Diethyldithiocarbamic acid is a chemical compound used in various laboratory applications. It is a white crystalline solid with a distinct odor. The core function of diethyldithiocarbamic acid is to act as a chelating agent, binding to metal ions to form stable complexes. This property makes it useful in analytical chemistry and various extraction processes.

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Lab products found in correlation

6 protocols using diethyldithiocarbamic acid

Purification of Commercial hCP Enzyme

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The commercial hCP (Cabiochem) was further purified by gel filtration chromatography, using Superose 6 FPLC column (Pharmacia, Sweden). Salsolinol, azide, formate, mannitol, bathocuproine disulfonate, diethyldithio carbamic acid (DDC) and penicillamine (PA) were obtained from Sigma (St. Louis, MO, USA). Chelex 100 resin (sodium form) was obtained from Bio-Rad (Hercules, CA, USA). All solutions were treated with Chelex 100 resin to remove any traces of transition metal ions.

Kim S.S., Kang J.Y, & Kang J.H. (2016). Oxidative modification of human ceruloplasmin induced by a catechol neurotoxin, salsolinol. BMB Reports, 49(1), 45-50.

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Quantifying NADPH Oxidase Activity

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NADPH oxidase activities were assayed with lucigenin enhanced chemiluminescence method as previous described47 (link)48 (link)49 (link). Briefly, cells were suspended and lysed in NADPH lysis buffer (50 mM phosphate buffer, pH 7.0, 1 mM EGTA, 150 mM sucrose, and protease inhibitors). Total cell lysate containing 100 μg proteins were incubated with 10 μmol diethyldithiocarbamic acid (Sigma) at 37°C for 30 min. Then mixtures were treated with 5 μmol lucigenin (Sigma) and 0.1 μmol NADPH (Sigma) balanced with NADPH lysis buffer. After incubation for 10 min at 37°C in dark, the samples were detected with luminometer. Reactions were terminated with 10 μmol tiron (Sigma). The enzyme activity was expressed as relative light units/μg protein and relative fold changes were used to indicate the activity changes.

Gu Y., Lee W, & Shen J. (2014). Site-2 protease responds to oxidative stress and regulates oxidative injury in mammalian cells. Scientific Reports, 4, 6268.

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Vascular Function Modulator Assay

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Lead acetate Pb (CH₃COO)₂, L-phenylephrine hydrochloride, acetylcholine chloride, sodium nitroprusside, N-nitro-L arginine methyl ester, catalase, superoxide dismutase, diethyldithiocarbamic acid, apocynin and tetraethylammonium were purchased from Sigma-Aldrich (Sigma Chemical Co., USA). The salts and reagents used were of analytical grade and were from Sigma-Aldrich and Merck (Darmstadt, Germany).

Nunes K.Z., Nunes D.O., Silveira E.A., Cruz Pereira C.A., Broseghini Filho G.B., Vassallo D.V, & Fioresi M. (2015). Chronic Lead Exposure Decreases the Vascular Reactivity of Rat Aortas: The Role of Hydrogen Peroxide. PLoS ONE, 10(3), e0120965.

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Fabrication of Biocidal Cu2O-Infused Nonwoven Textiles

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Polypropylene (PP) nonwoven textiles (Kimtech Pure W4 Critical Task Dry Wipers) were purchased from Kimberly Clark. Within this fabric were holes that were approximately 1 mm in diameter (See Figure 1). Cu₂O particles (Chem Copp HP III Type UltraFine-5) were purchased from the American Chemet Corporation. Metrics of the particle size and chemical composition are given in the Supporting Material, Tables S1 and S2. Diethyldithiocarbamic acid (ACS reagent grade Sodium diethyldithiocarbamate trihydrate) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic Acid (Bicinchoninic Acid Disodium Salt > 98% purity) was purchased from Tokyo Chemical Industry (Tokyo, Japan). One hundred percent ethanol (EtOH ACS grade) was purchased from VWR. Deionized (DI) water was from a Milli-Q Reference (MilliporeSigma) water purification system.

Benmamoun Z., Wyhopen T., Li Y, & Ducker W.A. (2022). Mechanism and Efficacy of Cu2O-Treated Fabric. Antibiotics, 11(11), 1633.

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Superoxide Anion Production in Mouse Aortas

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Superoxide anion production in mouse aortas were measured by lucigenin-enhanced chemiluminescence assay. Briefly, dissected mouse aortas were incubated in Krebs-HEPES solution containing (in mmol L⁻¹): 99 NaCl, 4.7 KCl, 25 NaHCO₃, 1 KH₂PO₄, 20 Na-HEPES, 1.2 MgSO₄, 2.5 CaCl₂ and 11 glucose, supplemented with diethyldithiocarbamic acid (1 mmol L⁻¹; Sigma-Aldrich) and β-NADPH (0.1 mmol L⁻¹; Sigma-Aldrich) at 37 °C for 45 min. The aorta were then transferred to vials containing lucigenin (10 μmol L⁻¹; Sigma-Aldrich) in Krebs-HEPES solution [29 (link)]. Repeated measurements were recorded by the GloMax^® 20/20 Luminometer (Madison, WI, USA) in 1 min intervals for 10 min. The amount of superoxide anion produced was presented as real numbers in relative light units per mg of dry tissue [30 (link)].

Cheng C.K., Shang W., Liu J., Cheang W.S., Wang Y., Xiang L., Lau C.W., Luo J.Y., Ng C.F., Huang Y, & Wang L. (2022). Activation of AMPK/miR-181b Axis Alleviates Endothelial Dysfunction and Vascular Inflammation in Diabetic Mice. Antioxidants, 11(6), 1137.

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Hormone Application in Tissue Culture

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Before making plates, appropriate amount of individual hormone solutions were added into the culture medium around 50 °C. Jasmonic acid (Sigma), (S)-(+)-Ibuprofen (Sigma), Paclobutrazol (Sigma), Gibberellic acid (Sigma), Ancymidol (Sigma) were dissolved in ethanol. Aminoethoxyvinyl glycine hydrochloride (Sigma), AgNO₃ (Sinopharm Chemical Reagent Co., LTD), 2,3,5-triiodobenzoic acid (Sigma) and Diethyldithiocarbamic acid (Sigma) were dissolved in ddH₂O.

Zheng H., Pan X., Deng Y., Wu H., Liu P, & Li X. (2016). AtOPR3 specifically inhibits primary root growth in Arabidopsis under phosphate deficiency. Scientific Reports, 6, 24778.

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