Anti-Tgfβ3 (Cat#AF-243-NA) and anti-EphB2 (Cat#AF467) were obtained from R&D Systems (Minneapolis, MN). The TgfβrI Kinase Inhibitor VI (SB431542) was from Calbiochem (EMD Millipore Cat#616465) (Billerica, MA). EphB2 ectodomain Fc fusion protein was from R&D Systems (Cat #467-B2) (Minneapolis, MN). IgG Fc protein was from Calbiochem (EMD Millipore Cat #401104) (Billerica, MA). Recombinant Tgfβ3 was purchased from R&D systems, CA. For Immunofluorescence, primary antibodies used (and their source) included the following:, E-Cadherin, Desmoplakin, and Plakoglobin (kindly provided by Dr. James Wahl, University of Nebraska Medical Center), Vimentin (Sigma-Aldrich, MO), Fibronectin (Abcam, MA), ZO-1 (Invitrogen, CA). All antibodies and inhibitors were used at the concentration and time point recommended by the respective manufacturer/provider.
Recombinant tgfβ3
Manufactured by R&D Systems
Sourced in Germany
Recombinant Tgfβ3 is a laboratory reagent produced by R&D Systems. It is a protein that belongs to the transforming growth factor beta (TGF-β) family. Tgfβ3 is involved in various cellular processes, but its core function is to serve as a tool for research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Vimentin, by Merck Group (1 mentions)
E cadherin, by Merck Group (1 mentions)
Anti ephb2, by R&D Systems (1 mentions)
Fibronectin, by Abcam (1 mentions)
Biotinylated secondary antibody, by Vector Laboratories (1 mentions)
Abc kit, by Vector Laboratories (1 mentions)
Beta glo assay system, by Promega (1 mentions)
Cell proliferation reagent wst 1, by Roche (1 mentions)
β gal staining kit, by Roche (1 mentions)
Anti sox9 c 20 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti type x collagen col 10 antibody, by Merck Group (1 mentions)
Polypropylene tube, by Thermo Fisher Scientific (1 mentions)
3 protocols using recombinant tgfβ3
1
Immunofluorescence Assay for Cell-Cell Junctions
2
Chondrogenic Differentiation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were from Sigma (Munich, Germany) unless otherwise indicated. Recombinant TGF-β3 was from R&D Systems (Wiesbaden, Germany). The anti-SOX9 (C-20) antibody was from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type-II collagen (II-II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, USA), and the anti-type-X collagen (COL-10) antibody from Sigma. Biotinylated secondary antibodies and the ABC kit were from Vector Laboratories (Grünberg, Germany). The β-gal Staining Kit and the Cell Proliferation Reagent WST-1 were from Roche Applied Science (Mannheim, Germany). The Beta-Glo® Assay System was from Promega (Mannheim, Germany). The type-II collagen ELISA (Human COL2A1) was purchased at Cusabio (College Park, MD, USA).
3
Chondrogenic Differentiation of Cell Suspensions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell suspensions of 2.5 × 105 cells per 15 ml polypropylene tube (Thermo Fisher Scientific, Waltham, MA) were centrifuged at 150 × g for 5 min, and the cell pellets were cultivated in complete chondrogenic medium with 10 ng/ml recombinant TGF-β3 (R&D Systems, Minneapolis, MN) for 4 weeks as described previously [9 (link)]. After fixing with 4% PFA in phosphate-buffered saline (PBS), the cell pellets were embedded in paraffin and sectioned into 5 μm thick slices. Alcian blue staining was performed to identify a cartilaginous matrix. Total RNA was isolated from each culture after 3 days.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!