Tetrachloroauric acid

Manufactured by Merck Group

Sourced in United States, Germany, France, Switzerland

Tetrachloroauric acid is a chemical compound with the formula HAuCl4. It is a yellow, crystalline solid that is soluble in water. Tetrachloroauric acid is commonly used as a precursor in the synthesis of other gold compounds.

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Lab products found in correlation

Sodium borohydride, by Merck Group (29 mentions) Silver nitrate, by Merck Group (22 mentions) L ascorbic acid, by Merck Group (12 mentions) Ascorbic acid, by Merck Group (11 mentions) Hydrochloric acid (hcl), by Merck Group (8 mentions) Cetyltrimethylammonium bromide, by Merck Group (8 mentions) Ethanol, by Merck Group (7 mentions) Polyvinylpyrrolidone, by Merck Group (5 mentions) Sodium hydroxide (naoh), by Merck Group (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Hydrogen peroxide, by Merck Group (4 mentions) Trisodium citrate, by Merck Group (4 mentions) Sodium citrate, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Bovine serum albumin (bsa), by Merck Group (4 mentions) Sulfuric acid, by Merck Group (3 mentions) Toluene, by Merck Group (3 mentions) Tetrahydrofuran, by Merck Group (3 mentions) Sodium citrate tribasic dihydrate, by Merck Group (3 mentions) Phosphate buffered saline (pbs), by Merck Group (3 mentions)

Close spelling variants of this product

Tetrachloroauric acid trihydrate (HAuCl4·3H2O, Tetrachloroauric(III) acid trihydrate Tetrachloroauric acid (HAuCl4·3H2O) Tetrachloroauric (III) acid Tetrachloroauric (III) acid (HAuCl4) Tetrachloroauric(III) acid (HAuCl4·3H2O, Tetrachloroauric acid (HAuCl4) Tetrachloroauric(iii) acid trihydrate (HAuCl4·3H2O, Tetrachloroauric acid trihydrate Tetrachloroauric(

77 protocols using tetrachloroauric acid

Aptamer-Based Electrochemical Biosensor

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Di-sodium hydrogen phosphate (Na₂HPO₄), sodium dihydrogenphosphate dihydrate (NaH₂PO₄·2H₂O), sodium chloride (NaCl), L-lysine hydrochloride (C₆H₁₄N₂O₂·HCl), tetrachloroauric acid (HAuCl₄), sulfuric acid (H₂SO₄), and potassium chloride (KCl) were acquired from Merck (Milan, Italy). potassium hexacyanoferrate (II) trihydrate (K₄[Fe(CN)₆]·3H₂O), potassium hexacyanoferrate (III) (K₃[Fe(CN)₆]), Trizma^® Base (Tris), magnesium chloride hexahydrate (MgCl₂·6H₂O), β-lactoglobulin B (β-LG), and 6-mercapto-1-hexanol (MCH) were obtained from Sigma-Aldrich (Milan, Italy).
The thiolated aptamer used, modified in the 3’-end with methylene blue (MB), had the following sequence: 5’-HS-CGA CGA TCG GAC CGC AGT ACC CAC CCA CCA GCC CCA ACA TCA TGC CCA TCC GTG TGT G-MB-3’, and was obtained from Biomers.net (Ulm, Germany).
All the reagents used were of analytical grade and used as they were received, without the need of further purification. Milli-Q water (18 MΩ) was used to prepare all the solutions.

Amor-Gutiérrez O., Selvolini G., Fernández-Abedul M.T., de la Escosura-Muñiz A, & Marrazza G. (2020). Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes. Sensors (Basel, Switzerland), 20(8), 2349.

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Synthesis of Gold Nanoparticles

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Tetrachloroauric acid (HAuCl₄, ≥99%), hexadecyltrimethylammonium bromide (CTAB for molecular biology ≥99%), 1-decanol (n-decanol, 98%), silver nitrate (AgNO₃, ≥99.0%), hydrochloric acid (HCl, 37%), sodium borohydride (NaBH₄), L-ascorbic acid (AA, ≥99%), tetrahydrofuran (THF, anhydrous, ≥99.9%), dodecanethiol (DDT, ≥98%), toluene (anhydrous, ≥99.8%), dodecylamine (≥99%), cyclohexane (99.5%), formaldehyde (37 wt% in H₂O, 10–15% methanol as stabilizer) and ethanol (95%) were purchased from Merck (Darmstadt, Germany). All chemicals were used without further purification. Milli-Q water was used in all experiments.

Szustakiewicz P., Kowalska N., Bagiński M, & Lewandowski W. (2021). Active Plasmonics with Responsive, Binary Assemblies of Gold Nanorods and Nanospheres. Nanomaterials, 11(9), 2296.

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Quantifying Virus Titers in ZZ-R 127 Cells

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Ten-fold virus dilutions were used to infect triplicate wells of confluent ZZ-R 127 cells pre-seeded in 6-well tissue culture plates. Following adsorption at 37 °C for 1 h, the inoculum was removed and 2 mL of indubiose (MP Biomedicals, Santa Ana, CA, USA) overlay was added. Following incubation (48 h, 37 °C, 5%CO₂), cells were fixed by the addition of 10% Tetrachloroauric acid (Merck) for 30 min. indubiose plugs were then removed and cells were stained with methyl blue solution (PBS, 10% formaldehyde, 10% of 1% methyl blue in ethanol) prior to determining plaque forming units/mL (PFU/mL).

Harvey Y., Jackson B., Carr B.V., Childs K., Moffat K., Freimanis G., Tennakoon C., Juleff N, & Seago J. (2022). An Improved αvβ6-Receptor-Expressing Suspension Cell Line for Foot-and-Mouth Disease Vaccine Production. Viruses, 14(3), 621.

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Virus Titration in ZZ-R 127 Cells

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Ten-fold virus dilutions were used to infect triplicate wells of confluent ZZ-R 127 cells that were pre-seeded in 6-well tissue culture plates. Following adsorption at 37 °C for 1 h, the inoculum was removed and 2 mL of indubiose (MP Biomedicals, Santa Ana, CA, USA) overlay was added. After a further 48 h incubation at 37 °C, the cells were fixed by the addition of 10% tetrachloroauric acid (Merck, Gillingham, UK) for 30 min. indubiose plugs were then removed, and the cells were stained with methyl blue solution (PBS, 10% formaldehyde, 10% of 1% methyl blue in ethanol) prior to the determination of the plaque forming units/mL (PFU/mL).

Childs K., Jackson B., Harvey Y, & Seago J. (2022). Trans-Encapsidation of Foot-and-Mouth Disease Virus Genomes Facilitates Escape from Neutralizing Antibodies. Viruses, 14(6), 1161.

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Synthesis of Pharmaceutical Compounds

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For drug synthesis, chemicals of analytical grade were utilized. Sodium borohydride and tetrachloroauric acid were acquired from Merck. Nystatin, fluconazole and amphotericin B were obtained from Sigma-Aldrich.

Anwar A., Siddiqui R., Raza Shah M, & Ahmed Khan N. (2019). Gold Nanoparticles Conjugation Enhances Antiacanthamoebic Properties of Nystatin, Fluconazole and Amphotericin B. Journal of microbiology and biotechnology, 29(1).

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Protein Quantification and Characterization

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Tetrachloroauric acid (HAuCl 4 •3H 2 O), sodium phosphate buffer, NaCl, Triton X-100, Tris-HCl, bicinchoninic acid protein assay kit, bovine serum albumin (BSA), nitrocellulose membranes, skim milk, Tween-TBS, DAB (3,3′-diaminobenzidine), NiCl 2 (nickel (II) chloride), H 2 O 2 (hydrogen peroxide), and nitric acid (HNO 3 ) were purchased from Sigma-Aldrich (St. Louis, MO), PGC1α primary PGC1 antibody PGC1 (Novus Biologicals, Littleton, CO). Aqua regia (mixture of concentrated nitric and hydrochloric acids in a ratio of 1:3) 20 kDa dialysis tubing was purchased from Spectrum Labs (Miami, FL).

Akkam N., Aljabali A.A.A., Akkam Y., Abo Alrob O., Al-Trad B., Alzoubi H., Tambuwala M.M, & Al-Batayneh K.M. (2023). Investigating the fate and toxicity of green synthesized gold nanoparticles in albino mice. Drug development and industrial pharmacy, 49(8).

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Coconut-Curcumin-Zebrafish Biomedical Study

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The coconut was collected from the local market in Cabanatuan city. The analytical grade tetrachloroauric acid was purchased from Sigma Aldrich, and no further treatment was applied. The Curcumin was purchased from Shaanxi Sangherb Biotech Inc, and the zebrafish from Central Luzon State University.

Perez K.S.M., & Abesamis M. (2021). Synthesis of Gold Nanoparticle using Coconut for Encapsulation of Curcumin. Oriental Journal Of Chemistry, 37(1), 116-119.

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Synthesis and Characterization of Metal Nanoparticles

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Sodium citrate, cobalt nitrate, chromium nitrate, nickel nitrate, aluminum nitrate, copper nitrate, and iron nitrate were obtained from Panreac (Barcelona, Spain); silver nitrate, sodium borohydride, and acetonitrile were obtained from Merck (Darmstadt, Germany); tetrachloroauric acid, polyvinylpyrrolidone, and ethylene glycol were purchased to Sigma-Aldrich (Madrid, Spain); and nitric acid (68%) and ascorbic acid were obtained from VWR (Lovaina, Belgium). All reagents were of analytical grade. Solutions were prepared using deionized water (18 MΩ, Milli-Q, Millipore, Bedford, MA, USA).

Ruíz del Portal-Vázquez P., López-Pérez G., Prado-Gotor R., Román-Hidalgo C, & Martín-Valero M.J. (2020). Citrate and Polyvinylpyrrolidone Stabilized Silver Nanoparticles as Selective Colorimetric Sensor for Aluminum (III) Ions in Real Water Samples. Materials, 13(6), 1373.

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Synthesis and Characterization of Gold Nanoparticles

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Tetrachloroauric acid (30% in HCl, 99.99%), 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol, sodium borohydride (≥98%), ascorbic acid (≥99%), silver nitrate (≥99%), 7-mercapto-4-methylcoumarin (≥97%), (3-aminopropyl)triethoxysilane (APTES) (≥98%), norfloxacin (≥98%), tetramethylthiuram disulfide (≥97%), ethanol (≥99.8%), toluene (≥99.7%), hydrochloric acid (≥37%), nitric acid (≥65%), sulphuric acid (≥95%), hydrogen peroxide (30 wt%) and ammonium hydroxide solution (≈29%) were purchased from Sigma Aldrich.
Microscopy cover glass slides 21 mm × 26 mm have been obtained from DEL Chimica and PDMS (Sylgard 184) was purchased from Dow Chemical.
Water was deionized and double distilled (ddH₂O).
All the glassware that came in contact with nanoparticles was always cleaned with aqua regia for 15 min, then washed three times with bi-distilled water for 3 min under sonication and dried in an oven for 1 h at 140 °C.

Parmigiani M., Albini B., Pellegrini G., Genovesi M., De Vita L., Pallavicini P., Dacarro G., Galinetto P, & Taglietti A. (2022). Surface-Enhanced Raman Spectroscopy Chips Based on Silver Coated Gold Nanostars. Nanomaterials, 12(20), 3609.

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Synthesis of Choline-Decorated Polymeric Nanoparticles

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Tetrachloroauric acid (HAuCl₄*3H₂O) ≥ 99.9%, molar weight: 393.83 g/mol; βCD (C₄₂H₇₀O₃₅) ≥ 98%, 1134.98 g/mol; (±) epichlorohydrin (EP, C₃H₅ClO) ≥ 99%, 92.52 g/mol; choline chloride (CC, C₅H₁₄ONCl) ≥ 98%, 139.62 g/mol; PhEA (1-phenylethan-2-amine, C₈H₁₁N) ≥ 99%, 121.18 g/mol, δ: 0.962 g/mL; PIP ((2E,4E)-5-(2H-1,3-benzodioxol-5-yl)-1-(piperidin-1-yl)penta-2,4-dien-1-one, C₁₇H₁₉NO₃) ≥ 97%, 285.3 g/mol; and sodium hydroxide (NaOH) ≥ 97%, 40.00 g/mol were provided by Sigma Aldrich (Saint Louis, MO, USA). Hydroxylamine hydrochloride (NH₂OH*HCl) ≥ 98%, 69.49 g/mol; hydrochloric acid (HCl) for analysis, 36.46 g/mol; and water (nanopure) were provided by Merck (Darmstadt, Germany).

Donoso-González O., Lodeiro L., Aliaga Á.E., Laguna-Bercero M.A., Bollo S., Kogan M.J., Yutronic N, & Sierpe R. (2021). Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring. Pharmaceutics, 13(2), 261.

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