Log In Sign up
The largest database of trusted experimental protocols

Cd4 t lymphocyte enrichment set

Manufactured by BD
Visit Supplier
Sourced in United States

The CD4 T Lymphocyte Enrichment Set is a laboratory product designed for the isolation and enrichment of CD4+ T lymphocytes from biological samples. The set contains reagents and materials necessary to perform this specific cell isolation procedure.

Automatically generated - may contain errors

Lab products found in correlation

Interleukin 2 (il 2), by Merck Group (2 mentions) Anti cd3 145 2c11, by BD (2 mentions) Anti cd28 37.51, by BD (2 mentions) Tgf β1, by Merck Group (2 mentions) Influx cell sorter, by BD (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Plate coated anti cd3e, by Thermo Fisher Scientific (1 mentions) Soluble anti cd28, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions)

7 protocols using cd4 t lymphocyte enrichment set

1

CD4+ T Cell Differentiation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4+ T cells were isolated from spleens by negative selection using the CD4 T Lymphocyte Enrichment Set (BD). Cells were stimulated for 96 h with 1 μg/ml anti-CD3 (145-2C11; BD) and 2 μg/ml anti-CD28 (37.51; BD) for polyclonal activation. Cells were differentiated to TH1 by supplementation with 10 ng/ml IL-12 and 10 μg/ml anti-IL-4 mAb (11B11). TH2 differentiation was induced by supplementation with 10 ng/ml IL-4 and 10 μg/ml anti-IFNγ mAb (XMG1.2). For TH17 conversion, cells were supplemented with 20 ng/ml IL-6, 10 ng/ml IL-23, 1 ng/ml TGFβ1, and 10 ng/ml IL-1β in the presence of 10 μg/ml anti-IFNγ mAb and 10 μg/ml anti-IL-4 mAb. To induce Treg polarization, cells were incubated with 10 ng/ml TGFβ1, 1 ng/ml IL-2, and 1 ng/ml all-trans retinoic acid (Sigma, St. Louis, MO, USA). For Ag-specific activation, CD4+ T cells from OT-II mice were incubated with 20 μM OVA323-339 in the presence of γ-irradiated CD4− splenocytes under Treg polarizing conditions as above. For assessment of Gal-8 binding, naïve CD4+ T cells (CD4+CD62LhiCD44lo) were prepared by FACS from spleens of B6 mice using an Influx Cell Sorter (BD).
Sampson J.F., Suryawanshi A., Chen W.S., Rabinovich G.A, & Panjwani N. (2015). Galectin-8 promotes regulatory T cell differentiation by modulating IL-2 and TGFβ signaling. Immunology and cell biology, 94(2), 213-219.
+ Open protocol
+ Expand
2

Analyzing HBZ-Tg T Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
To measure the proliferation of CD4+ T cells of HBZ-Tg mice, we isolated murine splenic CD4+ T cells using the CD4 T Lymphocyte Enrichment Set (BD Biosciences, San Jose, CA, USA). Murine splenic dendritic cells were isolated from collagenase-digested low-density cells using the Dendritic Cell Enrichment Set (BD Biosciences). Purified CD4+ T cells were labeled with 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA). Labeled CD4+ T cells of HBZ-Tg, non-Tg and tax-Tg mice (2×105 cells/well) were cultured with or without dendritic cells (1×104 cells/well) from non-Tg mice for three days with soluble anti-CD3 antibody (30 ng/mL) stimulation in round-bottomed 96-well plates. CFSE dilution was analyzed by flow cytometry. For TIGIT/CD155 and PD-1/PD-L1 proliferation assays, HBZ or empty vector transduced cells (see below) were labeled with CellTrace Violet (Invitrogen) and stimulated with anti-CD3/CD155.Fc or anti-CD3/PD-L1.Fc or anti-CD3/control.Fc-coated beads at a bead-to-cell ratio of 1:1 for three days. Dye dilution was analyzed by flow cytometry.
Kinosada H., Yasunaga J.I., Shimura K., Miyazato P., Onishi C., Iyoda T., Inaba K, & Matsuoka M. (2017). HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathogens, 13(1), e1006120.
+ Open protocol
+ Expand
3

Differentiation and Cytokine Analysis of CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
OT-II CD25−CD4+ T cells (2 × 105 cells/ml) purified from the indicated mouse strains using CD4 T lymphocyte enrichment set (BD Bioscience) were stimulated with 1 μg/ml OT-II peptide (ISQAVHAAHAEINEAGR) in the presence of irradiated total splenocytes (2 × 106 cells/ml). The cytokine and antibody mixtures used for differentiation of CD4 cells to each TH cell subset were described previously 49 (link). Cytokine production after incubation ×5 d was analyzed by ELISA, according to manufacturer’s instructions (RD systems).
Leavenworth J.W., Verbinnen B., Yin J., Huang H, & Cantor H. (2014). A p85α–osteopontin axis couples the ICOS receptor to sustained Bcl-6 expression by follicular helper and regulatory T cells. Nature immunology, 16(1), 96-106.
+ Open protocol
+ Expand
4

Differentiation and Cytokine Analysis of CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
OT-II CD25−CD4+ T cells (2 × 105 cells/ml) purified from the indicated mouse strains using CD4 T lymphocyte enrichment set (BD Bioscience) were stimulated with 1 μg/ml OT-II peptide (ISQAVHAAHAEINEAGR) in the presence of irradiated total splenocytes (2 × 106 cells/ml). The cytokine and antibody mixtures used for differentiation of CD4 cells to each TH cell subset were described previously 49 (link). Cytokine production after incubation ×5 d was analyzed by ELISA, according to manufacturer’s instructions (RD systems).
Leavenworth J.W., Verbinnen B., Yin J., Huang H, & Cantor H. (2014). A p85α–osteopontin axis couples the ICOS receptor to sustained Bcl-6 expression by follicular helper and regulatory T cells. Nature immunology, 16(1), 96-106.
+ Open protocol
+ Expand
5

Enrichment of CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Negative selection of CD4+ T cells was performed by using the CD4 T Lymphocyte Enrichment Set (BD IMag) according to the manufacturer's instructions. The cell number was determined by staining with Türks Blue. 2×105 cells were employed to determine CD4+ T cell purity by flow cytometry using anti-CD3 and anti-CD4 antibodies. A purity of more than 90% was routinely achieved.
Abramowski P., Otto B, & Martin R. (2014). The Orally Available, Synthetic Ether Lipid Edelfosine Inhibits T Cell Proliferation and Induces a Type I Interferon Response. PLoS ONE, 9(3), e91970.
+ Open protocol
+ Expand
6

Isolation and Activation of Mouse CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were removed from sacrificed mice and single cell suspension was prepared by squeezing on a cell strainer (70 µm, BD Biosciences, San Jose, CA). CD4+ cells were isolated using CD4 T Lymphocyte Enrichment Set (BD Biosciences) and were stimulated with plate-coated anti-CD3e (50 ng/well) and soluble anti-CD28 (1 µg/mL, e-Biosciences) in round-bottomed 96-well plates. Twenty four hours later, the cells were harvested and stained with 10 µM 2,7-dichloro-fluorescein diacetate (DC-FDA, Sigma, St. Louis, MO) for 30 min at 37°C. Then the cells were washed with PBS and were immediately measured for DC-FDA fluorescence using FACSCalibur (BD Biosciences).
Kim H.R., Lee A., Choi E.J., Hong M.P., Kie J.H., Lim W., Lee H.K., Moon B.I, & Seoh J.Y. (2014). Reactive Oxygen Species Prevent Imiquimod-Induced Psoriatic Dermatitis through Enhancing Regulatory T Cell Function. PLoS ONE, 9(3), e91146.
+ Open protocol
+ Expand
7

CD4+ T Cell Differentiation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4+ T cells were isolated from spleens by negative selection using the CD4 T Lymphocyte Enrichment Set (BD). Cells were stimulated for 96 h with 1 μg/ml anti-CD3 (145-2C11; BD) and 2 μg/ml anti-CD28 (37.51; BD) for polyclonal activation. Cells were differentiated to TH1 by supplementation with 10 ng/ml IL-12 and 10 μg/ml anti-IL-4 mAb (11B11). TH2 differentiation was induced by supplementation with 10 ng/ml IL-4 and 10 μg/ml anti-IFNγ mAb (XMG1.2). For TH17 conversion, cells were supplemented with 20 ng/ml IL-6, 10 ng/ml IL-23, 1 ng/ml TGFβ1, and 10 ng/ml IL-1β in the presence of 10 μg/ml anti-IFNγ mAb and 10 μg/ml anti-IL-4 mAb. To induce Treg polarization, cells were incubated with 10 ng/ml TGFβ1, 1 ng/ml IL-2, and 1 ng/ml all-trans retinoic acid (Sigma, St. Louis, MO, USA). For Ag-specific activation, CD4+ T cells from OT-II mice were incubated with 20 μM OVA323-339 in the presence of γ-irradiated CD4− splenocytes under Treg polarizing conditions as above. For assessment of Gal-8 binding, naïve CD4+ T cells (CD4+CD62LhiCD44lo) were prepared by FACS from spleens of B6 mice using an Influx Cell Sorter (BD).
Sampson J.F., Suryawanshi A., Chen W.S., Rabinovich G.A, & Panjwani N. (2015). Galectin-8 promotes regulatory T cell differentiation by modulating IL-2 and TGFβ signaling. Immunology and cell biology, 94(2), 213-219.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!