3 aminopropyl trimethoxysilane

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(3-aminopropyl)trimethoxysilane is a silane coupling agent used to modify the surface properties of various materials, including glass, metal, and semiconductor surfaces. It is commonly used in the preparation of functional coatings, adhesives, and composites.

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(3-aminopropyl)trimethoxysilane (APTMS) (4 citations)

12 protocols using 3 aminopropyl trimethoxysilane

Sulfonated Polymer Composite Membranes

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A dimethylformamide solution of the MF-4SC perfluorosulfonic acid polymer in the lithium form (10 wt%, equivalent weight is 1100; Plastpolymer, Saint-Petersburg, Russia); Taunit S12 multiwall CNTs (outer diameter is 20–40 nm, inner diameter is 5–10 nm, obtained by CVD catalytic pyrolysis of hydrocarbons on Ni/Mg catalyst; NanoTechCenter, Tambov, Russia); nitric acid (special purity, >70% HNO₃, Chimmed, Moscow, Russia); p-toluenesulfonic acid (97.5%, Acros Organics, Geel, Belgium); D-glucose (Ph Eur, hydrated form, Merck, Darmstadt, Germany); ethanol (95%, Ferein, Minsk, Belarus); (3-aminopropyl)trimethoxysilane (97%, Alfa Aesar, Ward Hill, MA, USA); acetone (reagent grade, >99.75%, Chimmed, Moscow, Russia); hydrochloric acid (special purity, 35–38%, Chimmed, Moscow, Russia); potassium chloride (reagent grade, Chimmed, Moscow, Russia); sulfamethoxazole (4-amino-N-(5-methyl-3-isoxazolyl)benzenesulfonamide, 98%, Alfa Aesar, Ward Hill, MA, USA), trimethoprim (2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine, ≥99%, Alfa Aesar, Ward Hill, MA, USA); “Biseptol^® 120 mg” tablets (Adamed Pharma, Czosnów, Poland); deionized water (resistance 18.2 MΩ, pH 5.41 ± 0.05) were used.

Parshina A., Yelnikova A., Safronova E., Kolganova T., Kuleshova V., Bobreshova O, & Yaroslavtsev A. (2022). Multisensory Systems Based on Perfluorosulfonic Acid Membranes Modified with Functionalized CNTs for Determination of Sulfamethoxazole and Trimethoprim in Pharmaceuticals. Membranes, 12(11), 1091.

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Fabrication and Characterization of PFSA-CNT Composite

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A 10 wt% dimethylformamide (DMF) dispersion of the PFSA polymer with equivalent weight of 1100 in the Li⁺ form (MF-4SC, Plastpolymer, Saint-Petersburg, Russia) and multiwall CNTs (Taunit S12, NanoTechCenter, Tambov, Russia) were used. Nitric acid (special purity, >70%), acetone (reagent grade, >99.75%), hydrochloric acid (special purity, 35–38%), and potassium chloride (reagent grade) were bought in Chimmed (Moscow, Russia). D-glucose (Ph Eur, hydrated form, Merck, Darmstadt, Germany), p-toluenesulfonic acid (97.5%, Acros Organics, Geel, Belgium), ethanol (95%, Ferein, Minsk, Belarus), and 3-aminopropyl)trimethoxysilane (97%, Alfa Aesar, Ward Hill, MA, USA) were used. N-[(4-aminophenyl)sulfonyl]acetamide (>99%) and 4-aminobenzenesulfonamide (>99%) were supported from Sigma-Aldrich (Saint-Louis, MO, USA). Sulfacyl sodium-SOLOpharm eye drops (Grotex, Saint-Petersburg, Russia) were analyzed. The deionized water with resistance 18.2 MΩ and pH 5.41 ± 0.05 was obtained using a Simplicity^® Water Purification System (MerckMillipore, Darmstadt, Germany).

Parshina A., Yelnikova A., Safronova E., Kolganova T., Bobreshova O, & Yaroslavtsev A. (2023). Potentiometric Sensor Arrays Based on Hybrid PFSA/CNTs Membranes for the Analysis of UV-Degraded Drugs. Polymers, 15(12), 2682.

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Synthesis and Purification of Fluorescent Dye

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Iron (II) chloride, iron (III) chloride, suprapur nitric acid (65%) and suprapur sulphuric acid (96%)were purchased from Merck (purity levels 99%, Darmstadt, Germany). 3-Aminopropyltrimethoxysilane (APTMS) was purchased from Alfa Aesar (MA, USA).
Cardiogreen (indocyanine green) was obtained from Sigma-Aldrich (MO, USA). Vivaspin 20 centrifugal filters (5 kDa MW cut-off) were obtained from Sartorius (Goettingen, Germany). In all experiments, only ultra-pure water was used (18.2 MΩ, Rephile Bioscience and Technology, Shanghai, China).

Bilici K ., Atac N ., Muti A ., Baylam I ., Dogan O ., Sennaroglu A ., Can F , & Yagci Acar H . (2020). Broad spectrum antibacterial photodynamic and photothermal therapy achieved with indocyanine green loaded SPIONs under near infrared irradiation. Biomaterials science, 8(16).

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Multifunctional Silica Nanoparticle Synthesis

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Dimethyl
sulfoxide (DMSO, 99.9%), toluene
(99.5%), and hexane (98.5%) were purchased from BDH. Anhydrous toluene
(99.8%), Reagent Plus acetone (≥99%), Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene
glycol) (Pluronic P123, average M_n ∼5800),
tetraethyl orthosilicate (TEOS, 99.0%), N,N-diisopropylethylamine (DIPEA, 99.9%), hydrochloric acid
(37 wt %), ethanolamine (>98%), 5-amino-1-pentanol (95%), and 4-nitrobenzaldehyde
(4-NB, 98%) were obtained from Sigma Aldrich. (3-Aminopropyl)trimethoxysilane
(IPTMS, 97%), ammonium hydroxide (28 wt % in H₂O), 3-amino-1-propanol
(99%), and 4-amino-1-butanol (98%) were acquired from Alfa Aesar.
1,4-Dimethoxybenzene (DMB, >99%) was produced by Tokyo Chemical
Industry
(TCI), and ethanol (99.5%) was produced by VMR Koptec.

Xie J., Ellebracht N.C, & Jones C.W. (2019). Inter- and Intramolecular Cooperativity Effects in Alkanolamine-Based Acid–Base Heterogeneous Organocatalysts. ACS Omega, 4(1), 1110-1117.

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Synthesis and Characterization of PE/ZrP Nanocomposites

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Zirconyl chloride (ZrOCl₂·8H₂O, 98%, Sigma-Aldrich), phosphoric acid
(85%, EM Science), and all of the solvents are reagent grade and used
as received. Jeffamine M1000 with a molecular weight of 1000 g/mol
was donated by the Huntsman Corporation. 3-Aminopropyl trimethoxysilane
(≥97%) was purchased from Alfa Aesar. Octadecyl trimethoxysilane
(ODMS) (≥90%) was purchased from Gelest. MPE and PE used in
this study are listed in Table 1. PE1 has a more uniform structure with narrower molecular
weight distribution compared to PE2. As a result, PE1 is a simpler
matrix to study the PE/ZrP interfacial characteristics and their mechanical
property relationship.

Zhao M., Wu H.M., Chen H., Lai G.H., Zhu Z., Wu J.L., Kang W.H, & Sue H.J. (2023). Preparation of Polyethylene/α-Zirconium Phosphate Nanocomposites via a Well-Controlled Polyethylene-Grafted Interface. Langmuir, 39(16), 5803-5813.

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Synthesis of Nanomaterials from Molecular Precursors

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The nucleic acid bases adenine (A, 99%), thymine (T, 97%), cytosine (C, 98%+), and guanine (G, 98%), sodium citrate (99.0%), chloroauric acid (HAuCl 4 •3H 2 O, 99.99%), sodium perchlorate (NaClO 4 , 98%-102%), and (3-aminopropyl)trimethoxysilane (97%) were purchased from Alfa Aesar. Sodium silicate solution (27% SiO 2 ) was purchased from Sigma-Aldrich. All the chemicals were used directly without purification. Deionized Milli-Q water (∼18.2 MΩ cm) was used throughout the study.

Wen B.Y., Jin X., Li Y., Wang Y.H., Li C.Y., Liang M.M., Panneerselvam R., Xu Q.C., Wu D.Y., Yang Z.L., Li J.F, & Tian Z.Q. (2016). Shell-isolated nanoparticle-enhanced Raman spectroscopy study of the adsorption behaviour of DNA bases on Au(111) electrode surfaces. The Analyst, 141(12).

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Synthesis of Functionalized Silica Nanoparticles

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All chemicals and reagents were obtained from commercial suppliers and used without further purification. Cetyltrimethylammonium bromide (CTAB, 99%+), ammonium hydroxide (NH₄OH, 28~30% solution in water), tetraethyl orthosilicate (TEOS, 98%), and 3-aminopropyltrimethoxysilane (APTMS, 95%) were obtained from ACROS Organics™ (Waltham, MA, USA). Rhodamine isothiocyanate isomer (RITC, 70%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Orthopyridyldisulfide-polyethyleneglycol-N-hydroxysuccinimide (OPSS-PEG-NHS, MW 200) was purchased from Biochempeg (Watertown, MA, USA). n-Decane (C₁₀H₂₂, 99%) was purchased from Alfa Aesar (Ward Hill, MA, USA). 2-[Methoxy(polyethyleneoxy)6-9propyl]trimethoxysilane, tech-90 (PEG-silane, M.W. 460–590 g/mol) and trimethoxysilylpropyl modified (polyethyleneimine) (PEI-silane, M.W. 1500–1800 g/mol, 50% in isopropanol) were acquired from Gelest (Morrisville, PA, USA). 2-Iminothiolane hydrochloride (Traut’s reagent, ≥98% (TLC), powder) was purchased from Sigma-Aldrich. Ultrapure deionized (DI) water was generated using a Millipore Milli-Q Plus system (Merck Group, Darmstadt, Germany).

Chu Y.S., Wong P.C., Jang J.S., Chen C.H, & Wu S.H. (2022). Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering. Pharmaceutics, 14(5), 1078.

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Synthesis and Characterization of Silica-based Materials

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PdCl₂ (Silversalt, St. Petersburg, Russia, 98%), LiCl (TTX, Sergiev Posad, Russia, 98+), isopropanol (IPA, 99.5+%, Acros, Fair Lawn, NJ, USA), tetramethylorthosilicate (TMOS, Acros, 99%), (3-aminopropyl)trimethoxysilane (APTMS, Acros, 95%), N-(2-aminoethyl)-3-(trimethoxysilyl)propylamine (AEAPTMS, Sigma-Aldrich, St. Louis, MO, USA, 97%), (S)-(+)-2-phenylglycine methyl ester hydrochloride (Acros, 97%), L-phenylalanine methyl ester hydrochloride (Acros, 98%), methanol (Acros, 99.9%), hexene-1 (Acros, 97%), benzene (Sigma-Aldrich, 99.8%), acetophenone (Acros, 98%), benzaldehyde (Acros, 98+%), benzophenone (Acros, 99%), hexyne-1 (Acros, 98%), dicyclopentadiene (Merck, Darmstadt, Germany, 98%), cyclohexanone (Acros, 99.8%) and 1,4–dioxane (Acros, 99+%) were used without further purification. Lithium tetrachloropalladate (II) was prepared by mixing 1 mol of PdCl₂ and 2 mol of LiCl in methanol and stirring for 24 h at room temperature.

Sipyagina N.A., Vlasenko N.E., Malkova A.N., Kopitsa G.P., Gorshkova Y.E., Kottsov S.Y, & Lermontov S.A. (2024). Catalytically Active SiO2 Aerogels Comprising Chelate Complexes of Palladium. Molecules, 29(8), 1868.

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Functionalized Silica Nanoparticles for TNFα Delivery

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Dox hydrochloride, bovine serum albumin, adipic acid dihydrazide, paraformaldehyde, phosphate buffer saline (PBS, 0.1 M, pH 7.4), dichloromethane, m ethanol and acetic acid were purchased from Sigma-Aldrich. Tetraethoxysilane (TEOS), cetyltrimethylammonium bromide (CTAB), ethanol, ammonium hydroxide (30%) and 3-Aminopropyltrimethoxysilane (APTS) were purchased from Acros. n-Octane was purchased from Alfa Aesar. Silane-PEG-Maleimide (M.W. 2000) was purchased from Nanocs Inc. Recombinant Human TNFα protein was purchased from PeproTech Inc. McCoy’s 5A medium, DMEM medium, fetal bovine serum (FBS) and Trypsin-2.5% (W/V) EDTA solution (10X) were purchased from Gibco Co.

Hsia Y., Sivasubramanian M., Chu C.H., Chuang Y.C., Lai Y.K, & Lo L.W. (2023). A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis. International Journal of Nanomedicine, 18, 4253-4274.

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Synthesis and Characterization of Platinum-Based Nanoparticles

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Dimethyl sulphoxide (DMSO) was purchased from Panreac Quimica Sau. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Phosphate Buffered Saline (PBS), methanol/ formaldehyde, Potassium tetrachloroplatinate(II), trisodium 2-hydroxypropane-1,2,3-tricarboxylate and the 14kD dialysis tubing cellulose membrane were all purchased from Sigma Aldrich. Gold (III) chloride hydrate was purchased from Alfa Aesar, 3-aminopropyl-trimethoxysilane was obtained from Acros Organics. 2-propanol, dichloromethane and hexane were provided by Merck. 4chloro-quinazoline and Methanol-d4 was obtained from Biowest. For all the experiments Milli-Q H2O 18,2 mΩ was used.

Stavropoulou A.P., Theodosiou M., Sakellis E., Boukos N., Papanastasiou G., Wang C., Tavares A., Corral C.A., Gournis D., Chalmpes N., Gobbo O.L, & Efthimiadou E.K. (2022). Bimetallic gold-platinum nanoparticles as a drug delivery system coated with a new drug to target glioblastoma. Colloids and surfaces. B, Biointerfaces, 214.

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