The HPLC analytical-grade hexane, acetone, acetonitrile, ethyl acetate, methanol and analytical grade 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), hydrochloric acid, citric acid, sodium hydroxide, sodium chloride, aluminum chloride, ethanol, Folin–Ciocâlteu reagent, gallic acid, inulin were purchased by Sigma Aldrich (Darmstadt, Germany). For cromatographic analysis the following reagents were used: HCl ACS reagent (37%), acetic acid, methanol, ethyl acetate, acetonitrile, theaflavin, cafestol, procyanidin A1, procyanidin B1, (−)-epigallocatechin, catechin, caffeine, caffeic acid, ellagic acid, gallic acid, protocatechuic acid, trans-cinnamic acid, quercetin 3-glucoside, quercetin 3-D-galactoside, quercetin 3-β-D-glucoside, naringin, hesperidin, myricetin, apigenin, kaempferol, luteolin, and isorhamnetin (HPLC-grade), purchased from Sigma-Aldrich (Darmstadt, Germany). Other reagents such as sodium bicarbonate were purchased from Honeywell, Fluka (Seelze, Germany). The Lo. bifermentans MIUG BL 16 strain was from Microorganism Collection of Dunarea de Jos University (acronym MIUG, Galati, Romania). de Man, Rogosa and Sharpe agar (MRS agar) was purchased from Merck (Darmstadt, Germany).
Quercetin 3 d galactoside
Manufactured by Merck Group
Sourced in United States, Germany
Quercetin-3-D-galactoside is a chemical compound used in laboratory research. It is a flavonoid glycoside that can be isolated from various plant sources. This compound is commonly used as a standard reference material in analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Catechin, by Merck Group (4 mentions)
Gallic acid, by Merck Group (4 mentions)
Quercetin 3 glucoside, by Merck Group (4 mentions)
Kaempferol, by Merck Group (3 mentions)
Myricetin, by Merck Group (3 mentions)
Delphinidin 3 o glucoside, by Merck Group (3 mentions)
Malvidin 3 o glucoside, by Merck Group (3 mentions)
Citric acid, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Luteolin, by Merck Group (2 mentions)
Quercetin 3 β d glucoside, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Rutin, by Merck Group (2 mentions)
Cyanidin 3 o glucoside, by Merck Group (2 mentions)
Quercetin, by Merck Group (2 mentions)
Ellagic acid, by Merck Group (1 mentions)
Protocatechuic acid, by Merck Group (1 mentions)
Folin cioc lteu reagent, by Merck Group (1 mentions)
9 protocols using quercetin 3 d galactoside
1
HPLC Analysis of Bioactive Compounds
2
Phaseolus vulgaris Bioactive Compound Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Black-seeded common beans (Phaseolus vulgaris L. cv Negro de vaina morada) were cultivated in November 2018 and collected in March 2019 in Chiapas, Mexico. Seeds were stored at 4 °C until use. Ultrapure water, ethanol (≥99%), sodium carbonate, 2 N Folin–Ciocalteu reagent, gallic acid, chlorohydric acid, potassium chloride, sodium acetate, Amberlite® XAD-7HP, formic acid, n-hexane (≥95%), ethyl acetate (≥99.7%), methanol (≥99%), trifluoracetic acid (TFA), acetonitrile (≥99.9%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), 6-hydroxy-2,5,7,8-tetramethylchrome-2-carboxylic acid (Trolox®), potassium persulfate, sodium nitroprusside, Griess reagent, gallic acid (≥99%), genistein (≥98%), myricetin (≥96%), apigenin-7-glucoside (≥90%), epigallocatechin gallate (≥80%), kaempferol-3-glucoside (≥95%), quercetin-3-glucoside (≥90%), quercetin-3-D-galactoside (≥98%), delphinidin 3-O-glucoside (≥98%), malvidin 3-O-glucoside (≥98%), and petunidin-3-O-glucoside (≥98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). iNOS inhibitor screening kit (Fluorometric) was purchased from Biovision (Milpitas, CA, USA). COX-1 (ovine) and COX-2 (human) inhibitor screening assay kits were purchased from Cayman Co. (St. Louis, MO, USA). CO2 gases were obtained from Grupo Infra (Guadalajara, Jal., México).
3
Comprehensive Enzyme Inhibition Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Folin and Ciocalteu’s phenol reagent, 2-Aminoethyl diphenylborinate 97%, ABTS (2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid), DPPH: 2,2-Diphenyl-1-picrylhydrazyl, Trolox®: (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, MOPS: 3-(N-Morpholino) propanesulfonic acid, 4-Morpholinepropanesulfonic acid (≥99.5%), Orlistat: tetrahydrolipstatin (≥98%), DMSO: Dimethyl sulfoxide, α-Amylase from porcine pancreas (EC3.2.1.1), α-Glucosidase from Saccharomyces cerevisiae (EC3.2.1.20), lipase from porcine pancreas (EC3.1.1.3), Dipeptidyl Peptidase IV human (EC 3.4.14.5), quercetin-3-d -galactoside (≥98%), delphinidin 3-O-glucoside (≥98%), malvidin 3-O-glucoside (≥98%) and cyanidin 3-O-glucoside (≥98%), acarbose (≥95%), 3,5-Dinitrosalicylic acid (≥98%), p-Nitrophenyl-α-d -glucopyranoside (≥99%), catechin (99%), rutin (94%), gallic acid (97.5%), vanillin (99%), sodium nitroprusside dihyrate (≥99%), sitagliptin, tributyrin, sodium taurodeoxycholate hydrate, 4-Nitrophenol, tert-butanol and Griess reagent, were purchased from Sigma Aldrich (St. Louis, MO, USA). DPP-IV GLO® protease assay kit G8351 was purchased from Promega (Madison, WI, USA). All chemicals were analytical grade.
4
Bioactive Compounds in Black Beans
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The “black bean” (Phaseolus vulgaris L.) was cultivated in November 2018 and collected in March 2019 in Chiapas, Mexico. The grains were stored at 4 °C until use. Methanol (≥98%), ethanol (≥99%), sodium carbonate, Folin–Ciocalteu 2 N reagent, gallic acid, chlorohydric acid, potassium chloride, sodium acetate, Amberlite® XAD-7HP, formic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), 6-hydroxy-2, 5, 7, 8-tetramethylchrome-2-carboxylic acid (Trolox®), potassium persulfate, methanol mass grade, sodium phosphate dibasic, monopotassium phosphate, sodium hydroxide, tris base, 3,4-Dihydroxy-L-phenylalanine (L-DOPA), kojic acid, N-succinyl-ala-ala-ala-p-nitroanilide (SANA), dimethyl sulfoxide (DMSO), citric acid, (±) catechin hydrate, tyrosinase from mushroom (EC 1.14.18.1) and elastase from porcine pancreas (EC 3.4.21.36), quercetin-3-D-galactoside (≥98%), delphinidin 3-O-glucoside (≥98%), malvidin 3-O-glucoside (≥98%) and cyanidin 3-O-glucoside (≥98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Moringa oleifera Antioxidant Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dried leaves of Moringa oleifera L. were acquired from a herbalist’s shop. On the label, Italian origin (Salento, South Italy) was declared. Gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH radical), 2,4,6-tripyridyl-s-triazine (TPTZ), sodium carbonate (Na2CO3), aluminium chloride (AlCl3), iron(III) chloride hexahydrate (FeCl3·6H2O), Folin and Ciocalteu’s phenol reagent, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), kaempferol (≥90%), quercetin (≥95%), kaempferol-3-O-glucoside (≥95%), kaempferol-3-O-D-galactoside (≥90%), quercetin-3-D-galactoside (≥97.0%), quercetin-3-O-rhamnoside (≥78%), quercetin-3-β-D-glucoside (≥90%), quercetin 3-O-(6″-O-malonyl)-β-D-glucoside (≥85%) were from Sigma–Aldrich (Milan, Italy). HPLC grade and analytical grade solvents were acquired from VWR (Milan, Italy). Deionized water was obtained using a Milli-Q system (Millipore Corp, Billerica, MA, America).
6
Extraction and Analysis of Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents used for the extraction and analysis of phytochemicals (methanol, isopropanol, acetonitrile, water and formic acid) were LC-MS grade (67-56-1, 67-63-0, 75-05-8, 7732-18-5, 85178, respectively, Sigma-Aldrich, (St. Louis, MO, USA). Authentic standards for mangiferin, (+)-catechin, quercetin-3-D-galactoside, quercetin, gallic acid, (-)-epicatechin and quercetin-3-glucoside (M3547, 43412, 83388, Q4951, G7384, 1753, 16654, respectively, Sigma-Aldrich, St. Louis, MO, USA) were used. Kaempferol-3-O-glucoside (90242), 4-hydroxy-benzoic acid (99-96-7), caffeic acid (6034 S), 4-coumaric acid (6031 A), ferulic acid (6077 A), quercetin 3, 4-di-O-glucoside (1347 S), quercetin 3-D-O-galactoside (1027 S) standards were purchased from Extrasynthese (https://www.extrasynthese.com/ ). All reagents used for proteomic analysis were purchased from Sigma-Aldrich (St. Louis, MO, USA), except as otherwise specified in the corresponding section.
7
Quercetin Compounds in Golden Delicious Apples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Golden Delicious was used because it is the most produced green apple variety in our country, Turkey [19 ]. As it can be found in the market every season and due to some physical changes during storage, it was obtained from a constant supplier in Izmir during the analysis. The apples were used in the analysis as a whole with the skin and flesh. The average skin amounts of the apples were determined as 10%, and this weight was taken into consideration in all analyses.
Quercetin 3-D-galactoside (Sigma-Aldrich 00180585, St. Louis, MO, USA), quercetin-3-rhamnoside (Sigma-Aldrich 00740580), and quercetin-3-glucoside (Sigma-Aldrich 00140585), were purchased from St. Louis, Mo, USA, are the most common quercetin compounds in the Golden Delicious species, the amounts of 3 quercetin compounds in green apple were determined and used in rats’ feed.
Quercetin 3-D-galactoside (Sigma-Aldrich 00180585, St. Louis, MO, USA), quercetin-3-rhamnoside (Sigma-Aldrich 00740580), and quercetin-3-glucoside (Sigma-Aldrich 00140585), were purchased from St. Louis, Mo, USA, are the most common quercetin compounds in the Golden Delicious species, the amounts of 3 quercetin compounds in green apple were determined and used in rats’ feed.
8
Evaluation of Analgesic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following drugs and reagents were used: Indomethacin -Ind (Sigma-Aldrich Inc., St. Louis, MO, USA), morphine sulfate -Mor (Cristália, SP, Brazil), acetic acid and formalin (Merck KGaA, Darmstadt, Germany) . Dimethylsulfoxide (DMSO): physiological saline, 1:50, was used in all experiments as the control. Flavonoids and cinnamic derivatives were used as standards being chlorogenic acid, quercetin-3-Dgalactoside, quercetin-3-D-glucoside e isorhamnetin-3-O-galactoside (Sigma Aldrich®). All the solvents for Ultra-fast liquid chromatography (UFLC) were of HPLC grade. All other reagents were of analytical grade.
9
Determination of Fiber and Polyphenols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thermostable α-amylase (Novozymes, Bagsvaerd, Denmark) was used for digestion of starch. The reagents used to determine the content of neutral detergent fiber (NDF) were: sodium dodecyl sulfate (C12H25NaO4S, Sigma-Aldrich, Saint Louis, USA), neutral disodium versenate dehydrate (C10H14N2Na2O8*10H2O), disodium tetraborate decahydrate (Na2B4O7*10 H2O), disodium hydrogen phosphate (Na2HPO4) and ethylene glycol (Poch, Gliwice, Poland). Reagents used to determine the content of ADF were 1 N sulfuric acid (H2SO4, 1 N, Poch, Gliwice, Poland) and N-cetyl-N,N,N-trimethylammoniumbromid (C19H42BrN, Merck, Darmstadt, Germany). Reagents used to determine the content of ADL were: sulfuric acid (72%), and acetone (Poch, Gliwice, Poland). Determination of polyphenolic contents was performed using the reagents and standards of acetonitrile, methanol, 2,6-dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 4-hydrobenzoic acid, caffeic acid, catechin, chlorogenic acid, fagopyrin, ferulic acid, myricetin, gallic acid, isovanilic acid, isovitexin, kaempferol, luteolin, p-coumaric acid, procyanidin B2, quercetin, quercetin 3-D galactoside, rutin, syringic acid and vitexin, purchased from Sigma Aldrich (Steinheim, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!