To evaluate the inhibitory activity of the CM derivative against topo I and topo II enzymes, human DNA topoisomerase 1 ELISA kit (#MBS289119, MyBioSource, San Diego, CA, USA) and human DNA topoisomerase II ELISA kit (#EKN48879-96T, BIOMATIK, Wilmington, DE, USA) were utilized. Also, to evaluate the level of bax (proapoptotic factor) and bcl2 (antiapoptotic factor) proteins, (#E-EL-H0562, human bax ELISA kit, Elabscience, TX, USA) and (#E-EL-H0114, human bcl2 ELISA kit, Elabscience, Houston, TX, USA) were utilized, respectively. Cancer cells were treated with the IC50 of the CM derivative for 48 h. Then, untreated and treated cells were harvested followed by measurement of topo I, topo II, bax, and bcl2 according to manufacturer’s instructions. A standard curve was obtained, and the absorbance was measured at 450 nm. The concentration of topo I, topo II, bax, and bcl2 were calculated from the standard curve. The experiment was performed in triplicate.
E el h0114
Manufactured by Elabscience
Sourced in United States
E-EL-H0114 is a laboratory equipment product. It is designed for specific functions within a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information may be available from the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using e el h0114
1
Evaluating Apoptotic Regulators in Cancer Cells
2
Quantifying Bcl-2 and Hsp-70 Levels
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Hsp-70 (CAT
no. E-EL-H1863, Elabscience Biotechnology lnc.) and Bcl-2 (CAT no.
E-EL-H0114, Elabscience Biotechnology lnc.) levels were determined
by the ELISA method according to the manufacturer protocol. Briefly,
antigen (tissue homogenate) was added to a well plate, pre-coated
with a rat Bcl-2 primary antibody. Biotinylated detection antibody
specific for rat Bcl-2 and Avidin-horseradish peroxidase conjugates
were added sequentially to the well plate and incubated. Free components
were washed away. Afterward, substrate solution was added. The reaction
was stopped by adding the stop solution. The absorbance was estimated
by a micro plate ELISA reader (Biobase-EL 10A) at a wavelength of
450 nm.
no. E-EL-H1863, Elabscience Biotechnology lnc.) and Bcl-2 (CAT no.
E-EL-H0114, Elabscience Biotechnology lnc.) levels were determined
by the ELISA method according to the manufacturer protocol. Briefly,
antigen (tissue homogenate) was added to a well plate, pre-coated
with a rat Bcl-2 primary antibody. Biotinylated detection antibody
specific for rat Bcl-2 and Avidin-horseradish peroxidase conjugates
were added sequentially to the well plate and incubated. Free components
were washed away. Afterward, substrate solution was added. The reaction
was stopped by adding the stop solution. The absorbance was estimated
by a micro plate ELISA reader (Biobase-EL 10A) at a wavelength of
450 nm.
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