IL‐2 and IL‐2/harmine NPs were re‐suspended between 5 and 10 mg/ml in deionized H2O and their diameters measured by dynamic light scattering using the Malvern Panalytical Zetasizer Ultra system. To determine the stability of the colloidal dispersions, the Malvern Panalytical Zetasizer Ultra was also used to measure the zeta potential of NPs in suspension.
Zetasizer ultra system
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer Ultra system is a versatile laboratory instrument primarily used for the measurement of particle size, zeta potential, and molecular weight. The system utilizes dynamic light scattering (DLS) and electrophoretic light scattering (ELS) techniques to provide accurate and reliable data on the properties of samples in liquid or suspension.
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4 protocols using zetasizer ultra system
1
Characterizing IL-2 and IL-2/Harmine Nanoparticles
2
Characterizing IL-2 and IL-2/harmine Nanoparticles
3
Characterization of Magnetic Nanoparticles in Cell Culture
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The MFNP characterization was performed using cell culture under similar conditions, at a concentration of 10 µg Fe/mL dispersed in DMEN/F-12 medium, supplemented with 10% FBS, and 1% antibiotic-antimycotic solution.
The MFNP optical characteristics were measured using an RF-6000 spectrofluorophotometer (Shimadzu, Kyoto, Japan) and LabSolutions RF software (Shimadzu, Kyoto, Japan). MFNP were dispersed in supplemented medium enclosed in a quartz cuvette with 1 cm of the optical path. The 2D excitation spectrum graphics were obtained in the wavelength between 400 to 850 nm and emissions from 450 to 870 nm.
The hydrodynamic size of MFNP was determined using the dynamic light scattering (DLS) technique with the Zetasizer Ultra system (Malvern, Worcestershire, UK). The polydispersion curve of hydrodynamic size was obtained at an angle of 173°, with the number of averages set at 30 and acquisition time of 3 s in a fixed position at 37 °C, with 120 s of thermic equilibrium. The mean diameter and standard deviation were calculated by adjusting the experimental data to a log-normal distribution function.
The colloidal stability analysis of hydrodynamic diameter over time was performed with MFNP dispersed in the supplemented medium. The polydispersion curves were recorded every hour for 18 h at 37 °C.
The MFNP optical characteristics were measured using an RF-6000 spectrofluorophotometer (Shimadzu, Kyoto, Japan) and LabSolutions RF software (Shimadzu, Kyoto, Japan). MFNP were dispersed in supplemented medium enclosed in a quartz cuvette with 1 cm of the optical path. The 2D excitation spectrum graphics were obtained in the wavelength between 400 to 850 nm and emissions from 450 to 870 nm.
The hydrodynamic size of MFNP was determined using the dynamic light scattering (DLS) technique with the Zetasizer Ultra system (Malvern, Worcestershire, UK). The polydispersion curve of hydrodynamic size was obtained at an angle of 173°, with the number of averages set at 30 and acquisition time of 3 s in a fixed position at 37 °C, with 120 s of thermic equilibrium. The mean diameter and standard deviation were calculated by adjusting the experimental data to a log-normal distribution function.
The colloidal stability analysis of hydrodynamic diameter over time was performed with MFNP dispersed in the supplemented medium. The polydispersion curves were recorded every hour for 18 h at 37 °C.
4
Characterization of PS-b-PAA Micelles
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The hydrodynamic diameter and polydispersity of the PS-b-PAA micelles were determined via DLS on a Malvern Panalytical Zetasizer
Ultra system equipped with an avalanche photodiode detector (APD)
and a He–Ne laser (λ = 633 nm). Unless stated otherwise,
all polymer solutions were prepared in ethanol with a concentration
of 1.0 mg mL–1 and measured inside a 10 mm ×
10 mm quartz cuvette without prior filtering. Samples were recorded
in 5-fold in the noninvasive backscattering (NIBS) mode (173°
detector angle) at 25 °C after a 120 s equilibration time. Results
were analyzed using ZS Xplorer software (version 3.1). The reported
hydrodynamic diameters and standard deviations are the averaged values
based on these five consecutive measurements, while taking into account
the precision and accuracy of the device (±2%).
Ultra system equipped with an avalanche photodiode detector (APD)
and a He–Ne laser (λ = 633 nm). Unless stated otherwise,
all polymer solutions were prepared in ethanol with a concentration
of 1.0 mg mL–1 and measured inside a 10 mm ×
10 mm quartz cuvette without prior filtering. Samples were recorded
in 5-fold in the noninvasive backscattering (NIBS) mode (173°
detector angle) at 25 °C after a 120 s equilibration time. Results
were analyzed using ZS Xplorer software (version 3.1). The reported
hydrodynamic diameters and standard deviations are the averaged values
based on these five consecutive measurements, while taking into account
the precision and accuracy of the device (±2%).
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