All fungal strains (S1 Table ) were provided by the Westerdijk Fungal Biodiversity Institute. Conidia were harvested with a cotton swab after seven days of growth at 25°C on malt extract agar (MEA, Oxoid, Hampshire, UK) and suspended in 10 mL ice-cold ACES buffer (10 mM N(2-acetamido)-2-aminoethanesulfonic acid, 0.02% Tween 80, pH 6.8). The conidia suspension was passed through a syringe containing sterilized glass wool and washed twice with ACES buffer after centrifugation at 4°C for 5 min at 2,500 g. The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
B rker t rk haemocytometer
Manufactured by Avantor
The Bürker-Türk haemocytometer is a laboratory instrument used for the counting and analysis of cells, particularly blood cells. It consists of a glass slide with a defined grid pattern, which allows for the accurate quantification of cell populations within a specific volume of a sample.
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2 protocols using b rker t rk haemocytometer
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Fungal Strain Preparation for Bioassays
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Fungal Conidia Harvesting and Quantification
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All fungal strains (Table S1) were provided by the Westerdijk Fungal Biodiversity Institute.
Conidia were harvested with a cotton swab after seven days of growth at 25 °C on malt extract agar (MEA, Oxoid, Hampshire, UK) and suspended in 10 mL ice-cold ACES buffer (10 mM N(2acetamido)-2-aminoethanesulfonic acid, 0.02 % Tween 80, pH 6.8). The conidia suspension was passed through a syringe containing sterilized glass wool and washed twice with ACES buffer after centrifugation at 4 °C for 5 min at 2,500 g. The spore suspension was set to 2•10 8 spores mL -1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
Conidia were harvested with a cotton swab after seven days of growth at 25 °C on malt extract agar (MEA, Oxoid, Hampshire, UK) and suspended in 10 mL ice-cold ACES buffer (10 mM N(2acetamido)-2-aminoethanesulfonic acid, 0.02 % Tween 80, pH 6.8). The conidia suspension was passed through a syringe containing sterilized glass wool and washed twice with ACES buffer after centrifugation at 4 °C for 5 min at 2,500 g. The spore suspension was set to 2•10 8 spores mL -1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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