Human melanoma (BLM) cells [20 (
link)] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with
Nutrient Mixture F12 (Gibco, Merelbeke, Belgium), supplemented with 10 % fetal bovine serum (
FBS) (Hyclone, Thermo Scientific, MA, USA), 20 U/ml
penicillin-streptomycin (Gibco), 2 mM
l-glutamine (Gibco), and 10 mM
HEPES (Sigma-Aldrich®). Human pharynx squamous cell carcinoma (FaDu) cells (ATCC® HTB-43™, LGC Standards GmbH, Wesel, Germany) were cultured in
high-glucose DMEM (Sigma-Aldrich®, St. Louis, MO, USA), supplemented with 10 % (
v/v)
FBS (Sigma-Aldrich®) and 1 %
non-essential amino acids (Sigma-Aldrich®). Rat glioma (C6) cells (
ATCC® CCL-107™) were maintained in low-glucose DMEM (Sigma-Aldrich®) supplemented with 10 %
FBS. Cells were cultured in standard cell culture flasks in a humidified incubator at 5 % CO
2 and 37 °C.
Ultrasound experiments with BLM cells were performed in
OptiCells™ (Nunc, Thermo Scientific, MA, USA), wherein 1.3 × 10
6 cells were plated 1 day prior to the experiment. For ultrasound experiments with FaDu or C6 cells, 1 × 10
6 cells were seeded into CLINIcell® cell culture chambers (Mabio, Tourcoing, France) 2 days prior to the experiment, to ensure a confluent cell monolayer during the experiment. CLINIcells® were coated with Poly-
l-Lysine (Sigma-Aldrich®) before cell seeding for proper cell attachment.
Lammertink B.H., Deckers R., Derieppe M., De Cock I., Lentacker I., Storm G., Moonen C.T, & Bos C. (2017). Dynamic Fluorescence Microscopy of Cellular Uptake of Intercalating Model Drugs by Ultrasound-Activated Microbubbles. Molecular Imaging and Biology, 19(5), 683-693.
