Human upar immunoassay

Manufactured by R&D Systems

The Human uPAR Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure human urokinase-type plasminogen activator receptor (uPAR) levels in cell culture supernates, serum, and plasma. uPAR is a glycosylphosphatidylinositol (GPI)-anchored protein that binds to and regulates the activity of the urokinase-type plasminogen activator (uPA) system, which is involved in various physiological and pathological processes.

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Lab products found in correlation

Human total mmp 7 quantikine elisa kit, by R&D Systems (2 mentions)

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Quantikine Human uPAR Immunoassay (5 citations)

2 protocols using human upar immunoassay

Quantifying Secreted Biomarkers in Cells

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Following the manufacturer’s protocols, uPAR, MMP-7, and ET-1 secretion were evaluated by using a Human uPAR Immunoassay (Cat# DUP00 R&D), Human Total MMP-7 Quantikine ELISA Kit (Cat# DMP700, R&D), and ET-1 Human Endothelin-1 ELISA Kit (Car# CEK1146, Cohesion Biosciences), respectively. Briefly, 50 µL of conditioned medium obtained at different time points was added into each well of the 96-well plate pre-coated with anti-tag antibody by using the manufacturer’s instructions. The concentration of each desired protein in each sample was determined by interpolating the absorbance values against the standard curve that was calculated by recombinant proteins at gradient dilution.

Del Rio D., Masi I., Caprara V., Ottavi F., Albertini Petroni G., Salvati E., Trisciuoglio D., Giannitelli S.M., Bagnato A., Mauri E., Spadaro F, & Rosanò L. (2024). The β-arrestin1/endothelin axis bolsters ovarian fibroblast-dependent invadosome activity and cancer cell metastatic potential. Cell Death & Disease, 15(5), 358.

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Quantitative Protein Secretion Assay

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Following the manufacturer’s protocols, uPAR, MMP-7 and ET-1 secretion were evaluated by using a Human uPAR Immunoassay (Cat# DUP00 R&D), Human Total MMP-7 Quantikine ELISA Kit (Cat# DMP700, R&D), and ET-1 Human Endothelin 1 ELISA Kit (Car# CEK1146, Cohesion Biosciences), respectively, following manufacturer’s instructions. The concentration of each desired protein in each sample was determined by interpolating the absorbance values against the standard curve that was calculated by recombinant proteins at gradient dilution.

Del Rio D., Masi I., Caprara V., Spadaro F., Ottavi F., Strippoli R., Sandoval P., López-Cabrera M., Sainz de la Cuesta R., Bagnato A, & Rosanò L. (2021). Ovarian Cancer-Driven Mesothelial-to-Mesenchymal Transition is Triggered by the Endothelin-1/β-arr1 Axis. Frontiers in Cell and Developmental Biology, 9, 764375.

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