Anti ha tag c29f4

Cells modified with SB transposons were seeded in 12‐well plates. 24 h after transfection protein expression was induced by doxycycline (1 μg/mL). Next day cells were lysed in SDS Loading Buffer (0.35 M Tris·HCl, 35% (v/v) glycerol, 10% (w/v) SDS, 3.6 M β‐mercaptoethanol, 0.12 g/mL bromophenol blue) and denatured in 95°C for 7 min. Protein extracts were analyzed by Western blot. Following antibodies were used: mouse monoclonal anti‐FLAG (F3165, Merck), anti‐HA‐Tag (C29F4, Cell Signaling Technology), anti‐Lamin B1 (D9V6H, Cell Signaling Technology), anti‐GAPDH (D16H11, Cell Signaling Technology), anti‐IκBα (L35A5, Cell Signaling Technology), anti‐β‐actin (8H10D10, Cell Signaling Technology), anti‐GFP (A‐11122, Invitrogen) anti‐mouseHRP (#7076), and anti‐rabbit HRP (#7074) (Cell Signaling). Luminescence was detected using the Clarity Western ECL Substrate (Bio‐Rad) and recorded using the Fusion‐Fx documentation system (Vilber Lourmat).

Anti-RTEL1, produced in Rabbit (Sigma, Cat. No. HPA067329); Anti-RPA1, produced in Mouse (Sigma, Cat. No. SAB1406399); Anti-RPA 32 kDa subunit (9H8), produced in Mouse (Santa Cruz Biotechnology, Cat. No. sc-56770); Anti-HA tag (C29F4), produced in Rabbit (Cell Signalling Technology, Cat. No. 3724); Anti-gamma H2A.X (phospho S139) antibody [3F2], produced in mouse (Abcam, Cat. No. ab22551); Anti-beta Actin, produced in Mouse (Abcam, Cat. No. ab8226); Anti-Rabbit IgG, HRP-conjugate (Millipore, Cat. No. 12–348); Anti-Mouse IgG, HRP-conjugate (Cell Signalling Technology, Cat. No. 7076); Anti-Rabbit IgG, Alexa Fluor™ 488-conjugate (Invitrogen, Cat. No. A-11008); Anti-Rabbit IgG, Alexa Fluor™ 594-conjugate (Invitrogen, Cat. No. SA5-10040); Anti-Mouse IgG, Alexa Fluor™ 488-conjugate (Invitrogen, Cat. No. A-11001); Anti-Mouse IgG, Alexa Fluor™ 594-conjugate (Invitrogen, Cat. No. A-11032)