Ab15 basic

Manufactured by Thermo Fisher Scientific

The AB15 Basic is a laboratory pH meter that measures the pH of aqueous solutions. It features a large, easy-to-read LCD display and intuitive user interface. The device is powered by a rechargeable battery and comes with standard pH and temperature probes.

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Lab products found in correlation

Phrodo green am intracellular ph indicator, by Thermo Fisher Scientific (1 mentions) Ph calibration buffer kit, by Thermo Fisher Scientific (1 mentions) Brookfield digital viscometer model dv 2 version 2, by Ametek (1 mentions)

Close spelling variants of this product

Accumet Basic AB15 Accumet Basic AB15 pH meter

2 protocols using ab15 basic

Nanomaterials Impact on Tumor Microenvironment

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To estimate how nanoCaCO₃ were affecting the tumor microenvironment, pH measurements were made using 24 well plates. First, MDA-MB-231 cells were seeded in the wells at a concentration of 3.0 × 10⁶ cells mL⁻¹. The next day, the media was replaced with either plain media (control) or media containing 0.8 mg mL^-1 nanoCaCO₃ (experimental). After 3 more days, the media was collected, and a pH probe (AB15 Basic, ThermoFisher) was used to measure the pH of the media in the wells.
Additionally, the intracellular pH was determined using a pHrodo Green AM Intracellular pH Indicator (ThermoFisher) following the manufacture’s protocol. With this dye, higher fluorescent intensities indicate a lower pH. Therefore, to correlate fluorescent intensities with pH values, a pH Calibration Buffer Kit (ThermoFisher) was used.

Lam S.F., Bishop K.W., Mintz R., Fang L, & Achilefu S. (2021). Calcium carbonate nanoparticles stimulate cancer cell reprogramming to suppress tumor growth and invasion in an organ-on-a-chip system. Scientific Reports, 11, 9246.

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Measurement of Jejunal Digesta Viscosity

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Samples of jejunum digesta from 50 mL tubes were divided into 2 falcon tubes (15 mL) and centrifuged at 1,000 × g at 4 °C for 10 min to obtain the liquid phase. Then, the liquid phase was removed to an Eppendorf tube (2 mL) to centrifuge at 10,000 × g at 4 °C for 10 min. The supernatant obtained was transferred to another Eppendorf tube (1.5 mL) for further measurement. 0.5 mL of digesta supernatant was placed in the viscometer (Brookfield Digital Viscometer, Model DV-II Version 2.0, Brookfield Engineering Laboratories Inc., Stoughton, MA), set at 25 °C. The viscosity measurement was the average between 45.0/s and 22.5/s shear rates, and the viscosity values were recorded as apparent viscosity in millipascal-seconds (mPa s).
The pH was measured immediately after collecting using a pH meter (AB15 Basic, Thermo Fisher Scientific Inc., Waltham, MA), placed directly in the digesta.

Duarte M.E., Zhou F.X., Dutra WM J.r, & Kim S.W. (2019). Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs. Animal Nutrition, 5(4), 351-358.

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