Ubiquitin, Fluorescein isothiocyanate (FITC)-labeled Ubiquitin, and ATP were purchased from ThermoFisher. UBE2D3, UBE2R2, UBE2S, UBE2L3, and UBE2E1 were purchased from R&D Systems. Primary antibodies for UBA1a/b (Cell Signaling, 4891S), Poly-Ubiquitin (Cell Signaling, 3936S), UBE2C (Proteintech, 66087-1-Ig), UBE2D3 (Cell Signaling, 4330S), and UBE2R2 (Proteintech,14077-1-AP), and β-actin (Cell Signaling, 4970) were used at a concentration of 1:1000 and visualized using HRP-conjugated secondary antibodies (anti-rabbit [Cell Signaling, 7074S] or anti-mouse [Cell Signaling, 7076S]) at a concentration of 1:1000. HRP-conjugated antibodies were visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, WBKLS0500).
Ube2r2
Manufactured by Proteintech
UBE2R2 is a protein that functions as a ubiquitin-conjugating enzyme. It is involved in the ubiquitination process, which tags proteins for degradation by the proteasome.
Automatically generated - may contain errors
Lab products found in correlation
Ube2r2, by R&D Systems (2 mentions)
Adenosine triphosphate (atp), by Thermo Fisher Scientific (2 mentions)
Ubiquitin, by Thermo Fisher Scientific (2 mentions)
Ube2d3, by R&D Systems (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Immobilon western chemiluminescent hrp substrate, by Merck Group (2 mentions)
Fluorescein isothiocyanate fitc labeled ubiquitin, by Thermo Fisher Scientific (2 mentions)
Ube2l3, by R&D Systems (2 mentions)
Ube2e1, by R&D Systems (2 mentions)
Poly ubiquitin, by Cell Signaling Technology (2 mentions)
Ube2d3, by Cell Signaling Technology (2 mentions)
Ube2c, by Proteintech (2 mentions)
Ube2s, by R&D Systems (2 mentions)
2 protocols using ube2r2
1
Ubiquitin Conjugation Pathway Characterization
2
Ubiquitin Conjugation Pathway Analysis
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Ubiquitin, Fluorescein isothiocyanate (FITC)-labeled Ubiquitin, and ATP were purchased from ThermoFisher. UBE2D3, UBE2R2, UBE2S, UBE2L3, and UBE2E1 were purchased from R&D systems. Primary antibodies for UBA1a/b (Cell Signaling, 4891S), Poly-Ubiquitin (Cell Signaling, 3936S), UBE2C (Proteintech, 66087–1-Ig), UBE2D3 (Cell Signaling,4330S), and UBE2R2 (Proteintech,14077–1-AP), and β-actin (Cell Signaling, 4970) were used at a concentration of 1:1000 and visualized using HRP-conjugated secondary antibodies (anti-rabbit [Cell Signaling, 7074S] or anti-mouse [Cell Signaling, 7076S]) at a concentration of 1:1000. HRP-conjugated antibodies were visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, WBKLS0500).
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