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Fluorescein isothiocyanate fitc dye

Manufactured by Merck Group
Sourced in Germany

Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used in various research and diagnostic applications. It is a derivative of fluorescein, a yellow-green fluorescent compound. FITC has the ability to emit a bright green fluorescence when excited by a light source, typically in the blue or ultraviolet range. This property makes FITC a useful tool for labeling and detection of biomolecules, cells, and other biological structures.

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2 protocols using fluorescein isothiocyanate fitc dye

1

Ciprofloxacin-Loaded Mucoadhesive Nanocarriers

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Ciprofloxacin base (molecular weight: 331.34 Da), hyaluronic acid (LMW HA, MW 500 kDa), N-hydroxy succinimide (NHS), 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC),cremophore RH 40, tween 80, PEG 400, PEG 200, artificial mucin, oleic oil, linseed oil, caproyl 90, labrafil M, mineral oil, dulbecco’s modified eagle’s medium (DMEM), rhodamine, crystal violet, congo red, 4′,6-diamidino-2-phenylindole(DAPI), and fluorescein isothiocyanate (FITC) dye were purchased from Sigma Aldrich, Germany. Cystamine dihydrochloride was purchased from Avonchem, New Hampshire, UK) United Kingdom. All solvents used were of analytical grade.
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2

Synthesis and Labeling of Silica Particles

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Ethanol (puriss), silicone oil (10,000 cSt), silicone oil (50 cSt) and nile red (for microscopy) were purchased from Sigma-Aldrich. Glycerol (laboratory reagent grade ≥98 %) and cetyltrimethylammonium bromide (CTAB) (Pure) were purchased from Fisher Chemical. All chemicals were used as received. The silica particles (radius 14 nm) were made via the Stöber method by Andrew Schofield and fluorescently labeled with fluorescein isothiocyanate (FITC) dye (isomer I, Sigma-Aldrich) as described by Imhof et al. 42, 43
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