After stimulation, EoL-1 cells were washed in FACS buffer (phosphate-buffered saline (PBS) with 2% fetal bovine serum (FBS)) and incubated for 45 min at 4°C in the dark with the following antibodies: APC antihuman CD63 (BioLegend), APC antihuman CD69 (BioLegend), APC antihuman CD23 (BioLegend), and APC antihuman FcεR1α (BioLegend). Cell staining was assessed by flow cytometry on an Accuri C6 Flow Cytometer (BD Biosciences). FACS analysis was performed with FlowJo software (Tree Star Inc., Ashland, OR, USA).
Apc anti human cd63
Manufactured by BioLegend
Sourced in United States
The APC anti-human CD63 is a fluorochrome-conjugated antibody that specifically binds to the CD63 protein expressed on the surface of human cells. CD63 is a member of the tetraspanin family and is commonly used as a marker for late endosomes and lysosomes. This antibody can be used in flow cytometry and other immunoassays to detect and analyze CD63-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Apc antihuman cd23, by BioLegend (2 mentions)
Apc antihuman fcεr1α, by BioLegend (2 mentions)
Accuri c6 flow cytometer, by BD (2 mentions)
Apc anti human cd69, by BioLegend (1 mentions)
Pe anti human pdl1, by BioLegend (1 mentions)
Apc anti human cd86, by BioLegend (1 mentions)
Lsrfortessa flow cytometer, by FlowJo (1 mentions)
Alexa fluor 488 anti human cd16, by BioLegend (1 mentions)
Fmmyalf, by GenScript (1 mentions)
Brilliant violet 421 anti human cd45, by BioLegend (1 mentions)
Pe anti human cd62l, by BioLegend (1 mentions)
3 protocols using apc anti human cd63
1
Flow Cytometry Analysis of Immune Markers
2
Multiparameter Flow Cytometry Immunophenotyping
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Cell staining with PE anti-human Siglec-8 (BioLegend), PE anti-human CD (CD80) (BioLegend), APC anti-human FcεR1α (BioLegend), APC anti-human CD86 (BioLegend), APC anti-human CD23 (BioLegend), APC anti-human CD63 (BioLegend), PE anti-human PDL1 (BioLegend), PE anti-human IL-5 receptor alpha (IL-5Rα; R&D Systems) were assessed by flow cytometry on an Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and data analyzed with FlowJo software (Tree Star Inc., Ashland, OR, USA).
3
Neutrophil Activation and Surface Marker Analysis
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Isolated neutrophils were resuspended in PBS free from calcium and magnesium ions (PAA) at 10 × 106/mL and stimulated for 2 hours with 100 nM fMLF (Sigma-AldrichK) or 100 nM fMMYALF (GenScript) or pretreated for 10 minutes with 2.5 µM FPR1 antagonist CsH (Enzo Scientific) before stimulation. Neutrophils were incubated for 1 hour at 4°C with antibodies to Brilliant Violet 421 anti-human CD45 (Clone: HI30), APC/Fire 750 anti-human CD11b (activated) (Clone: CBRM1/5), Alexa Fluor 488 anti-human CD16 (Clone: 3G8), PE anti-human CD62L (Clone: DREG-56), and APC anti-human CD63 (Clone: H5C6) (BioLegend). Samples were washed and resuspended in 2% FCS and incubated briefly with DAPI (1:1000) before analysis using a BD Bioscience LSR Fortessa flow cytometer and FlowJo software (version 10.1).
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