Piccolo general chemistry 13 panel

Hematological assessments were performed on whole blood using an XT-2000iV analyzer (Sysmex America, Lincolnshire, IL, USA). Serum chemistries were measured with Piccolo General Chemistry 13 panels (Abaxis, Union City, CA, USA) on samples collected prior to and after exposure to MARV. Serum chemistries were determined using methods previously published (53 (link)). Results were correlated with liver and spleen densities and quantitatively obtained organ volumes. MARV titers in plasma were determined by plaque assay using grivet (Chlorocebus aethiops (Linnaeus, 1758)) kidney epithelial Vero E6 cells and by measuring MARV genome equivalents using real-time reverse transcription PCR, both as previously described (53 (link)).

Blood work was performed at each imaging time point. Hematological assessments were obtained using whole blood (XS100i; Sysmex America, Lincolnshire, IL, USA). Serum chemistries were measured with Piccolo General Chemistry 13 panels (Abaxis, Union City, CA, USA). A list of assessed blood variables can be found in Table 1. Viremia was also assessed at each imaging time point using real-time reverse transcription PCR (RT-qPCR) (45 (link)). Liver samples were collected at necropsy, weighed, and snap-frozen in a dry ice-ethanol bath. Ten percent (wt/vol) homogenates were made in phosphate-buffered saline, samples were clarified, and the supernatants were serially 10-fold diluted for titration. Titrations were performed on Vero E6 cells, overlaid with methylcellulose, incubated for 7 days, fixed and stained with a crystal violet/formalin solution for 1 h at room temperature, and enumerated.