Hsflp

Manufactured by Bloomington Drosophila Stock Center

Sourced in Austria

The HsFLP is a laboratory equipment used for genetic manipulation in Drosophila research. It functions as a site-specific recombinase, allowing for the controlled expression or deletion of target genes in Drosophila.

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Lab products found in correlation

13 protocols using hsflp

Autophagy Regulation in Drosophila

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MTD-GAL4, UASp-mCherry-Atg8a (y¹ w¹¹¹⁸; P{UASp-mCherry-Atg8a}2; Dr¹/TM3, Ser¹), UASp-GFP-mCherry- Atg8a (y¹ w¹¹¹⁸; P{UASp-GFP-mCherry-Atg8a}2, wdr24¹ (CG7609¹), UAS-Tsc1 RNAi (y¹ sc* v¹; P{TRiP.GL00012}attP2), HS-FLP; Ubi-GFP FRT82B/TM3 and Df(3R)BSC547 lines were obtained from the Bloomington Stock Center. The mio¹, mio², seh1^Δ15, nprl ^RNAi and nprl3 ^RNAi lines were described previously [18 ,64 (link)]. GFP-Lamp1 (w¹¹¹⁸; P{W+, Tub>GFP-Lamp1}1/CyO; TM6b, Hu boss¹/Sb boss¹) was kindly provided by Helmut Kramer (UT Southwestern). Nanos-Gal4 (P{NANOS GAL4 VP-16}, yw; D/TM3, Ser, Sb) was kindly provided by Sharon Bickel (Dartmouth College). atg7^d77/Cyo-GFP and atg7^d14/CyO-GFP were kindly provided by Thomas P. Neufeld (University of Minnesota) [41 (link)]. All fly stocks were maintained on JAZZ-mix Drosophila food (Fisher Scientific) at 25℃.

Cai W., Wei Y., Jarnik M., Reich J, & Lilly M.A. (2016). The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lysosome Function. PLoS Genetics, 12(5), e1006036.

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Genetic Screen of Hedgehog Pathway

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The RNAi lines that targeted ci (2125R-1), hib (9924R-1), rpb3 (7885R-1) and rpb7 (v100309) used in genetic screen were obtained from the National Institute of Genetics Stock Center (NIG), Japan and the Vienna Drosophila RNAi Center (VDRC), Austria. eyflp, hsflp and FRT82 flies were obtained from the Bloomington Stock Center. Flies of MS1096, GMR-Gal4, Gal80, HA-Ci^−3P, Fg/Myc-SPOP, Fg-SPOPΔ3box, Fg-SPOPΔMATH, Fg-SPOPΔBTB, Myc-mouse-Gli2 have been described.⁴⁰ (link)^,⁵⁴ (link)
hib^Δ6 is a hib mutant allele, whose coding sequence is replaced by the white gene. The transgenic flies of Fg-Rpb3, Fg-Rpb7, HA-Grim, HA-Hid, HA-Rpr, Fg-SPOP^Y87N, Fg-SPOP^F102C, Fg-SPOP^S119N, Fg-SPOP^F125L, Fg-SPOP^K129E, Fg-SPOP^W131G and Fg-SPOP^F133V were generated by injection of corresponding constructs into Drosophila embryos according to the methods described previously.⁵⁵ (link) The parental strain for all germline transformations is w1118. All stocks used in this study were maintained and raised under standard conditions.

Gao Y., Shan Z., Jian C., Wang Y., Yao X., Li S., Ti X., Zhao G., Liu C, & Zhang Q. (2023). HIB/SPOP inhibits Ci/Gli-mediated tumorigenesis by modulating the RNA Polymerase II components stabilities. iScience, 26(8), 107334.

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Drosophila Germline Development Protocols

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Drosophila were grown on corn flour and agar media with brewer’s yeast. All strains were grown at 25 °C. pgcGFP, pgc^Δ and pgc^ΔFRT fly lines were generated for this study. liprinγ^H1 and bam^Δ⁸⁶ flies were acquired from the Treisman Lab (Astigarraga et al., 2010 ) and McKearin Lab (McKearin and Ohlstein, 1995 (link)) respectively. nos-Gal4::VP16, UASCycB (Mathieu et al., 2013 (link)) and sco/CyO; MKRS/TM6 was acquired from the Lehmann lab. UASpCycT-nos3′UTR, UASpcdk9-nos3′UTR and Genomic pgc⁺ transgene was given to us by the Nakamura Lab (Hanyu-Nakamura et al., 2008 (link)). CycB², bamGFP, hsFLP, 42DFRT-GFP, CycA^[c03456], CycA^[03946] and CycA^[c8LR1] are available at Bloomington Drosophila Stock Center, Bloomington, IN (Wang and Lin, 2005 (link)). CycTRNAi (v103387) and cdk9RNAi (v103569) lines were acquired from VDRC stock center.

Flora P., Schowalter S., Wong-Deyrup S., DeGennaro M., Nasrallah M.A, & Rangan P. (2017). Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila. Developmental biology, 434(1), 84-95.

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Genetic Tools for Drosophila Research

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All UAS-sona RNAi lines and sona mutants generated in our lab and characterization of sona-Gal4 line (P{GawB}CG9850) are described^{28 (link)}. P{lacW}Diap1^j5C8/TM3 was used as Diap1-lacZ line^{57 (link),58 (link)}. UAS-Diap1, UAS-lacZ, rpr-lacZ, wg-lacZ, UAS-GFP, UAS-p35, hsFLP; FRT42D ubiGFP, FRT42D ubi-GFP, ptc-Gal4, en-Gal4, tubulin-Gal4 and apterous-Gal4 were obtained from Bloomington stock center. UAS-rpr RNAi was obtained from VDRC (101234). ci-Gal4 is described in^{59 (link)}. Fly cultures were carried out at 25 °C unless otherwise indicated.

Tsogtbaatar O., Won J.H., Kim G.W., Han J.H., Bae Y.K, & Cho K.O. (2019). An ADAMTS Sol narae is required for cell survival in Drosophila. Scientific Reports, 9, 1270.

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Drosophila Genetics: Analyzing prod Function

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All fly stocks were raised on standard Bloomington medium at 25°C. The following fly stocks were used: prod^U (BDSC42686), UAS-GFP-nls (BDSC4776), tub-gal4 (BDSC5128), hs-flp (BDSC6), FRT42D, Ubi-nls-GFP (BDSC5626), UAS-dcr-2 (BDSC24650), D1-GFP (BDSC50850), HP1-RFP (BDSC30562) and UAS-DIAP1 (BDSC6657) were obtained from the Bloomington Drosophila stock center. D1^LL03310 (DGRC140754) and FRT42D prod^k08810 (DGRC111248) were obtained from the Kyoto stock center. UAS-prod^RNAi (VDRCv106593) was obtained from the Vienna Drosophila stock center. nos-gal4 and bam-gal4 have been previously described (Chen and McKearin, 2003 (link); Van Doren et al., 1998 (link)). wor-gal4 was a kind gift from Cheng-Yu Lee. Prod-null clones (indicated by loss of GFP signal) were generated as follows – Testes from flies of the genotype hs-flp; FRT42D, Ubi-nls-GFP/FRT42D, prod^k08810 were dissected 48 hr following a 1 hr heat shock at 37°C. For embryo and larval development analysis, flies laid eggs on apple-agar at RT and development was assessed every 24 hr.

Jagannathan M., Cummings R, & Yamashita Y.M. (2019). The modular mechanism of chromocenter formation in Drosophila. eLife, 8, e43938.

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Drosophila Germline Development Protocols

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Genetic Screen Protocols for Drosophila

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For the genetic screens, flies were ordered from Tsinghua Fly Center, NIG-Fly, Vienna Drosophila RNAi Center, and FLYORF. The results of the screens are shown in Supplementary data S1. Tool strains used in this study: UAS-Dicer2 (a gift from T.Tabata); nos-Cas9 (34 (link)); vasa-GAL4 (35 (link)); balancers, w¹¹¹⁸, ZH-attP-86Fb, FRT42B, ubiGFP, FRT42B, FRT42D, ubiGFP, FRT42D, nos-GAL4, hs-FLP, nos-GAL4,UAS-FLP/TM6, Cre and da-Gal4 were ordered from Bloomington Drosophila Stock Center.
Cbc related flies: UAS-cbcRNAi (NIG#5970R-3) from NIG-Fly; UAS-cbcRNAi (v100686/v20998) from Vienna Drosophila RNAi Center. cbc^Q5stop and cbc^A37T from Bloomington Drosophila Stock Center; cbc^8fs5 was generated by CRISPR-Cas9 resulting in a 5 bp-deletion after the 8th amino acid. Cbc-HA was a genomicly tagged fly, in which 3 × HA was fused to cbc C terminus before stop codon by CRISPR-Cas9. Transgenic flies of UAS-Cbc, UAS-Cbc-flag, UAS-Cbc^A37T-flag, UAS-mCLP1 and UAS-hCLP1 were generated by inserting the pUASt-attB construct to attP landing site of ZH-attP-86Fb flies (36 (link)).

Wu J., Li X., Gao Z., Pang L., Liu X., Huang X., Wang Y, & Wang Z. (2021). RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis. Human Molecular Genetics, 30(17), 1569-1578.

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Drosophila Genetics: Strains and Protocols

Cited 2 times

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The flies were raised at 25°C unless otherwise mentioned.
The following flies were used in this study:
CantonS, FRT42B, FRT2A, Gal80/II, Gal80/III, FRT80, GMR-GFP, UAS-mCD8GFP, hs-FLP, ey3.5-FLP, GMR-FLP, tub-Gal80ts, elav-Gal4, sev-Gal4, LGMR-Gal4, m∂0.5-Gal4, ro-tau-LacZ, Rh6-EGFP, PanR7-Gal4 were obtained from Bloomington Drosophila Stock Center. sens-Gal4/CyO and sens-Gal4/TM6 were gift from Bassem Hassan. sequoia⁵ is a previously generated Sequoia loss-of-function allele (Petrovic and Hummel, 2008 (link)). UAS-Sequoia was obtained from Jay Brenman. UAS-Capricious, UAS-Capricious^ID(intracellular deletion), Capricious^C18fs FRT2A and caps-LacZ^nls flies were kindly provided by Akinao Nose. GMR-gogo was a generous gift from Takashi Suzuki. PanR8-Gal4 was a gift from Claude Desplan. UAS-Capricious^RNAi was obtained from VDRC (VDRC Transformant ID No. 27097). PM181-Gal4 and CadN⁴⁰⁵FRT40 (Lee et al., 2001 (link)) were used in Sequoia analysis, Early Dm8 labelling OK371-VP16AD/CyO; ortC2-Gal4DBD/TM2, adult Dm8 specific OrtC1-3 LexA DBD, OrtC2B dVP16AD/CyO (Ting et al., 2014 (link)) and syn-GRASP constructs UAS-Syb::spGFP1-10 and LexAop spGFP11::CD4/TM2 (Karuppudurai et al., 2014 (link)) were used in syb-GRASP experiments.

Kulkarni A., Ertekin D., Lee C.H, & Hummel T. (2016). Birth order dependent growth cone segregation determines synaptic layer identity in the Drosophila visual system. eLife, 5, e13715.

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Genetic Tools for Drosophila Morphogenesis

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btl^LG19, bnl^P1, Df(2L)Exel6029, Df(3L)ri79c, Df(X)N73, da-GAL4, en-GAL4, UAS-myr-GFP, UAS-lifeact-GFP, AyGAL-FRT; UAS-lacZ, and hsFLP were obtained from Bloomington. btl-GAL4 was provided by S. Hayashi. UAS bnl^A1-1 and UAS bnl^A1-1 were provided by M. Krasnow. sage-GAL4 was created by our lab (Chung et al., 2009 (link)).

Cheng Y.L, & Andrew D.J. (2015). Extracellular Mipp1 activity confers migratory advantage to epithelial cells during collective migration. Cell reports, 13(10), 2174-2188.

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Genetic Tools for Drosophila Neural Development

Cited 3 times

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Fly strains used in this study include Oregon R, Ase-Gal4 (Zhu et al., 2006 (link)), Ase-Gal80 (Neumüller et al., 2011 (link)), brat^DG19310, brat^k06028 and brat¹¹ (Komori et al., 2014 (link)), erm¹ and erm² (Weng et al., 2010 (link)), erm-flag (Janssens et al., 2014 (link)), erm-lacZ and UAS-aPKC^CAAX (Haenfler et al., 2012 (link)), pnt^Δ88 (Morimoto et al., 1996 (link)), trx^Z11 (Tie et al., 2014 (link)), and Wor-Gal4 (Lee et al., 2006 (link)). The following stocks were obtained from the Bloomington Drosophila Stock Center: Elav-GAL4, Act-FRT-Stop-FRT-GAL4, ash2¹, btd^XA, FRT19A, FRT2A, FRT82B, GMR85C07-GAL4 (Btd-GAL4), hs-flp, P(EP)G4226, pros¹⁷, UAS-pnt_RNAi (TRiP.JF02227), UAS-pnt_RNAi (TRiP.HMS01452), trx^E2, tubP-Gal80, tubP-Gal80^ts, UAS-Dcr-2.D, UAS-mCD8-GFP, and UAS-trr_RNAi (TRiP.JF03242). We obtained the following stocks from the Vienna Drosophila RNAi Center UAS-ash2_RNAi (100718), UAS-dSet1_RNAi (40683), UAS-pnt_RNAi (7171), UAS-rbbp5_RNAi (106139), UAS-trx_RNAi (108122), and UAS-wds_RNAi (105371). UAS-HA-btd, UAS-HA-pntP1, UAS-rbbp5^FL-myc, and UAS-rbbp5^SG-myc were generated in this study by cloning the cDNA cloned into p{UAST}attB vector. The transgenic fly lines were generated via ϕC31 integrase-mediated transgenesis (Bischof and Basler, 2008 (link)). The rbbp5 null allele was generated by imprecisely excising the P(EP)G4226 element.

Komori H., Xiao Q., Janssens D.H., Dou Y, & Lee C.Y. (2014). Trithorax maintains the functional heterogeneity of neural stem cells through the transcription factor Buttonhead. eLife, 3, e03502.

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