Recombinant cdk1 cyclin b complex

Manufactured by New England Biolabs

The Recombinant CDK1/cyclin B complex is a purified protein product from New England Biolabs. It is composed of the cell cycle regulatory proteins Cyclin-Dependent Kinase 1 (CDK1) and Cyclin B. The complex plays a central role in the regulation of the cell cycle, particularly in the transition from G2 phase to mitosis.

Automatically generated - may contain errors

Lab products found in correlation

Kinase buffer, by New England Biolabs (2 mentions) Gstrap ff affinity columns, by GE Healthcare (2 mentions) Cdk1 cyclin b, by Sino Biological (2 mentions) γ 32p atp, by PerkinElmer (2 mentions)

Close spelling variants of this product

Cdk1–cyclin B Recombinant CDK1

2 protocols using recombinant cdk1 cyclin b complex

In Vitro Phosphorylation of MST2

Check if the same lab product or an alternative is used in the 5 most similar protocols

GST-tagged MST2 or MST2-S385A (cloned in pGEX-5X-1) was bacterially expressed and purified on GSTrap FF affinity columns (GE Healthcare) following the manufacturer’s instructions. GST-MST2 (1 μg) was incubated with 5-10 U recombinant CDK1/cyclin B complex (New England Biolabs) or 50-100 ng CDK1/cyclin B (SignalChem) in kinase buffer (New England Biolabs) in the presence of 5 μCi γ-³²P-ATP (3000 Ci/mmol, PerkinElmer) as we previously described [15 (link)]. Active CDK2, CDK5, p38, JNK1, JNK2, MEK1, ERK1, and PLK1 kinases were also purchased from SignalChem.

Chen X., Chen Y, & Dong J. (2016). MST2 Phosphorylation at Serine 385 in Mitosis Inhibits its Tumor Suppressing Activity. Cellular signalling, 28(12), 1826-1832.

+ Open protocol

+ Expand

Phosphorylation Assay for GST-PBK

Check if the same lab product or an alternative is used in the 5 most similar protocols

GST-tagged PBK and PBK-4A were cloned in pGEX-5X-1 and proteins were bacterially expressed and purified on GSTrap FF affinity columns (GE Healthcare) following the manufacturer’s instructions. GST-PBK (0.5–1 μg) was incubated with 10 U recombinant CDK1/cyclin B complex (New England Biolabs) or 100 ng CDK1/cyclin B (SignalChem) in kinase buffer (New England Biolabs) in the presence of 5 μCi γ-³²P-ATP (3000 Ci/mmol, PerkinElmer). Phosphorylation (³²P incorporation) was visualized by autoradiography followed by Western blotting or detected by phospho-specific antibodies.

Stauffer S., Zeng Y., Zhou J., Chen X., Chen Y, & Dong J. (2017). CDK1-mediated Mitotic Phosphorylation of PBK is Involved in Cytokinesis and Inhibits its Oncogenic Activity. Cellular signalling, 39, 74-83.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!