DNA extraction was undertaken with an automated DNA extraction method (X-tractor Gene; QIAGEN, Hilden, Germany). A validated in-house real-time multiplex PCR assay was performed to detect Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, and M. genitalium.19 (link) Those DNA extracts with inconclusive M. genitalium results on the in-house PCR assay, were re-tested using a commercial M. genitalium real-time PCR assay (Sacace Biotechnologies, Como, Italy) targeting the DNA subunit B of M. genitalium.13 (link) Human immunodeficiency virus screening was performed on sera (prepared from whole blood) by the use of two rapid tests, namely the DetermineHIV 1/2 assay (Abbott Laboratories, Japan) and Unigold™ assay (Trinity Biotech PLC, Ireland).
Determine hiv 1 2 assay
Manufactured by Abbott
Sourced in Japan, United States
The Determine HIV-1/2 assay is a rapid qualitative immunochromatographic test that detects antibodies to HIV-1 and HIV-2 in human serum, plasma, or whole blood samples.
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Lab products found in correlation
7 protocols using determine hiv 1 2 assay
1
Multiplex PCR for STI Detection
2
HIV Rapid Test and Typing
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Screening for HIV was done with a rapid test in the clinic (Determine HIV-1/2 assay, Abbott laboratories, Tokyo, Japan). HIV type discrimination was performed with Genie III HIV-1/HIV-2 (Bio-Rad, Steenvorde, France) [28] .
3
HIV Screening and CD4 Cell Counting Protocol
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HIV screening was conducted with a rapid test (Determine HIV-1/2 assay, Abbott Laboratories, Abbott Park, IL, USA), and confirmation and discrimination were performed using the SD Bioline HIV 1/2 3.0 rapid test (Standard Diagnostics Inc., Kyonggi-do, South Korea). During 2012, the SD Bioline HIV 1/2 3.0 was gradually replaced by the First Response HIV card 1-2.0 (PMC Medical, Mumbai, India). CD4 cell counts were measured by flow cytometry using Partec CyFlow® SL_3 (Cyflow SL, Partec, Munster, Germany).
4
Venous Blood Sampling and Metabolic Screening
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Fasting venous blood samples were collected by standardized aseptic technique and were transported to a laboratory for analysis within two hours of collection. Glucose was measured immediately on site by a glucometer. Total cholesterol, high-density lipoprotein, and triglyceride concentration were measured using Cobas c111 automated chemistry analyzer (Roche Diagnostics GmbH, Mannhein, Germany). The HIV status was determined using the rapid testing algorithm which included the Determine HIV-1/2 assay (Abbott Laboratories, Illinois, United States of America) for screening and the HIV-1/2 STAT-PAK Dipstick assay (Chembio Diagnostic Systems Inc., New York, USA) for confirmation of HIV status.
5
HIV Negative Women Screening Protocol
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All women included in the study were HIV seronegative in the previous HIVsurvey, and all women were HIV seronegative when retested in the current study. Screening for HIV was done using a rapid test (Determine HIV-1/2 assay, Abbott Laboratories).
6
CD4 Cell Counts and HIV Screening Protocol
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CD4 cell counts were performed since April 2007 by flow cytometry using Partec CyFlow® SL_3 (Cyflow SL, Partec, Munster, Germany) at the National Public Health Laboratory. HIV screening was done with a rapid test in the clinic (Determine HIV-1/2 assay, Abbott Laboratories, USA) and confirmation and discrimination using SD Bioline HIV 1/2 3.0 (Standard Diagnostics Inc, Kyonggi-do, South Korea). Since June 2012, the rapid test First Response HIV Card 1-2.0 (PMC Medical, Mumbai, India) has also been used for HIV type discrimination.
7
HIV Screening Algorithm Across Four Cities
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Screening for HIV was performed according to national algorithms [18 –21 ]. All four study cities first used the Determine HIV 1/2 assay (Abbott Laboratories, Chiba, Japan). Positive results were confirmed using the Bioline HIV-1/2 3.0 assay (SD, Gyeonggi-do, Republic of Korea) in Abidjan, Bamako, and Ouagadougou, or the First Response HIV-1/2 assay (Premier Medical Corporation, Mumbai, India) in Lomé. Samples with discordant results were tested a third time using the HIV 1/2 Stat-Pak assay (Chembio Diagnostics, New York, USA) in Abidjan, the First Response HIV-1/2 assay (Premier Medical Corporation, Mumbai, India) in Bamako, the Inno-Lia HIV I/II Score assay (Fujirebio, Zwijnaarde, Belgium) in Lomé, or a Western Blot assay in Ouagadougou.
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