Poly adp ribose polymerase parp sc 7150

Rabbit polyclonal antibody against EV-A71 3A was prepared as described (Tang et al., 2007 (link)). Mouse monoclonal antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G8795) and Flag (F1804) were purchased from Sigma-Aldrich (St Louis, MO, USA). Rabbit monoclonal antibodies against phospho-eIF2α (#3597) and PKR (Ab32052, against amino acids 50–150 of human PKR) were obtained from Cell Signaling (Beverly, MA, USA) and Abcam (Cambridge, UK), respectively. Mouse monoclonal antibody against green fluorescent protein (GFP) (SC-9996) and rabbit polyclonal antibodies against eIF2α (SC-11386) and poly (ADP-ribose) polymerase (PARP) (SC-7150) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal antibody against EV-A71 3C and rabbit polyclonal antibody against phospho-PKR were obtained from GeneTex (Irvine, CA, USA). Poly(I:C) and 2-aminopurine (2-AP) was purchased from Invivogen (San Diego, CA, USA). Staurosporine was purchased from Sigma-Aldrich.

The human wild-type Nanog (#28221) expression vector was acquired from Addgene (Cambridge, MA, USA). All chemicals were purchased from Sigma (München, Germany). Primary antibodies used in this study: β-actin (NB600-501), Akt (sc-8312), p-Akt (sc-33437), GSK3β (sc-8257), p-GSK3β (sc-11757), Nrf2 (sc-722), caspase 3 (sc-7148) and poly (ADP-ribose) polymerase (PARP) (sc-7150) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); SOD1 (#2770S) and Sirt1 (GTX61042) were purchased from GeneTex (Irvine, CA, USA); tau (05-348), p-tau (MAB5450) and p-Tyr (05-321) were obtained from Millipore (Bedford, MA, USA); Nanog (#4903), IRS-1 (194320) and p-IRS-1 (05–1087) were purchased from Cell Signaling Technology (Danvers, MA, USA). Aβ_1-42 was synthesized by LifeTein (Somerset, NJ, USA) and soluble Aβ oligomers were prepared according to our previously described [17 (link)]. Briefly, Aβ was first dissolved in hexafluoroisopropanol (HFIP), distributed in aliquots, dried and stored at −80 °C. The day before the experiment, Aβ was dissolved in sterile dimethyl sulfoxide (DMSO), then diluted with PBS (pH 7.4) at a final concentration of 100 μM, vortexed and incubated at 4 °C for 24 h.