Human gastric cancer cells SGC7901 and BGC-823 were purchased from the American Type Culture Collection (Maryland, USA) and maintained in 1640 complete medium (Gibco, CA, USA) supplemented with 10% fetal calf serum (Gibco, CA, USA) at 37° C in 5% CO2 atmosphere. β-catenin inhibitor β-catenin-IN2 (C-IN2) was purchased from MedChemExpress (N.J, USA). 5-FU was purchased from Sangon (Shanghai, China). Type I collagen and collagenase were purchased from Thermo (MA, USA). Other reagents were purchased from Solarbio (Beijing, China) and of HPLC standard.
1640 complete medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
1640 complete medium is a cell culture media product manufactured by Thermo Fisher Scientific. It is designed to support the growth and maintenance of a variety of cell types in vitro. The media provides the necessary nutrients, vitamins, and other components required for cell proliferation and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Collagen type 1, by Thermo Fisher Scientific (1 mentions)
Sgc 7901, by American Type Culture Collection (1 mentions)
Bgc 823, by American Type Culture Collection (1 mentions)
Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)
Anti cd3 okt3, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Solarbio (1 mentions)
Streptomycin, by Solarbio (1 mentions)
Microcentrifuge tube, by Eppendorf (1 mentions)
Ribociclib, by Selleck Chemicals (1 mentions)
Pemetrexed, by MedChemExpress (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
Nci h1650, by American Type Culture Collection (1 mentions)
Ag490, by Merck Group (1 mentions)
14 protocols using 1640 complete medium
1
Gastric Cancer Cell Culture and Treatments
2
Gastric Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SGC7901 human gastric cancer cell line, BGC823 human gastric cancer cell line and BJ cells human normal fibroblast cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA). All cell lines were maintained with 1640 complete medium (Gibco, Grand Island, NY) supplemented with 10% fetal calf serum (Gibco), at 37°C with 5% CO2 atmosphere. Female nude mice (6-8 weeks) were purchased from Beijing HFK Bioscience (Beijing, China) and fed in SPF experimental animal rooms.
3
Splenocyte CFSE Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1 ml volume of splenocytes was added in a fresh 15 ml tube and the tube was laid horizontally, 100 μl PBS was added to the non-wetted portion of the plastic at the top of the tube. 0.22 μl of the 5 mM stock of CFSE (C34554, life technology) was resuspended in the PBS, the tube was capped and quickly inverted several times and vortexed. The tube was incubated for 5 min at room temperature and washed twice to quench the unbound CFSE by diluting with ten volumes of PBS containing 5%(v/v) FBS and finally cells were resuspended in 1640 complete medium (Gibco) containing 10%(v/v) FBS and antibiotics at a concentration of 1 × 106 cells ml−1.
4
Isolation and Activation of Human PBMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human peripheral blood mononuclear cells (PBMCs) from healthy honors were isolated using lymphocyte separation solution (TBD, LTS1077, China) according to the manufactures’ instructions.33 (link) PBMCs were stained with 0.2 µM carboxyfluorescein succinimidyl esteractivated (CFSE) (eBioscience, 65-0850-84, USA), and then activated with 100 units IL-2 (PeproTech, 200-02-100, USA), 1 µg/mL anti-CD28 (CD28.2, eBioscience, USA), and 1 µg/mL anti-CD3 (OKT3, eBioscience, USA) stimulatory antibodies. Subsequently, the activated PBMCs were seeded into 48-well plates (4×105 cells per well) and then cultured in vehicle medium and the supernatants of KYSE70 cells pretreated with or without 10 µM 3, 5-diiodotyrosine for 48 hours. Above medium was Roswell Park Memorial Institute (RPMI) 1640 complete medium (Gibco, Grand Island, USA) supplemented with 100 µg/mL streptomycin (Solarbio, China), 100 U/mL penicillin (Solarbio, China) and 10% fetal bovine serum (FBS, BI, USA). After cells were cultured for 3 days. The intracellular cytokine staining experiment were performed as described in ex vivo assay. The proliferation of CD8+ T cells and proportion of IFN-γ+CD8+ T cells were analyzed by flow cytometry.
5
Cell Lines for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human ccRCC cell line, 786-O, the human RCC cell lines, ACHN, A498, and GRC-1, the normal human renal tubular epithelial cell line, HK-2, the human prostate cancer cell lines, PC3 and Du145, and the human colorectal cancer cell line, SW480, were obtained from the Laboratory of Pathology, West China Medical School, Sichuan University (Chengdu, China). Following cell dissociation and propagation, the 786-O, A498, ACHN, and GRC-1-1 cell lines were cultured (37°C) and grown in Roswell Park Memorial Institute (RPMI) medium using 1640 complete medium (Gibco®; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The GRC-1 RCC line was established at the Institute of Urology, Peking University (Beijing, China), was first reported by Ding et al (14 ), and has been subsequently used in numerous studies (15 (link),16 (link)). PC3 and Du145 cells were cultured (37°C) in Dulbecco’s modified Eagle’s medium (DMEM) complete medium (Gibco®; Thermo Fisher Scientific, Inc.), whereas HK-2 cells were cultured (37°C) in F-12 Complete™ medium (Gibco®; Thermo Fisher Scientific, Inc.) in microcentrifuge tubes (Eppendorf, Stevenage, UK) in a humidified incubator in an atmosphere of 5% CO2 and 95% air.
6
Lung Adenocarcinoma Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human lung adenocarcinoma cell lines A549, HCC827, NCI-1395, and NCI-H1650 were obtained from the American Type Culture Collection (ATCC). PC9 and NCI-H1975 cells were provided by Dr Fan (Department of Thoracic Surgery, Fourth Military Medical University, Xi’an, China). All cells were grown in Roswell Park Memorial Institute (RPMI) 1640 complete medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA), 100 U/mL penicillin (Thermo Fisher Scientific, MA, USA), and 100 ug/mL streptomycin (Thermo Fisher Scientific, MA, USA). All cells were cultured in a humidified atmosphere with 5% CO2 at 37°C. Cell lines were routinely authenticated, and mycoplasma tested. Ribociclib was purchased from Selleck, China, and pemetrexed was from Med Chem Express, China. Drugs were dissolved in dimethyl sulfoxide (DMSO, Sigma, USA) and stored at -20°C.
7
let-7a miRNA Transfection in PC12 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PC12 cells at 1 × 105/mL were seeded in a 24-well plate and incubated with 1640 complete medium (Gibco) containing 10% fetal bovine serum for 24 hours. At 80% confluence, cells were transfected with let-7a mimic or inhibitor and its negative control (Ribo Guangzhou, China), all at 20 nM (n = 6), using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). After transfection cells were cultured for 48 hours.
8
Induction of Experimental Autoimmune Encephalomyelitis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK, with the purity >95%) was synthesized by Xi’an Lianmei (China). PTX was purchased by Alexis (Germany). Mycobacterium tuberculosis H37RA was provided by Difco (USA). CFA was purchased from SigmaAldrich (USA). Sterile PBS was purchased from Difco (USA). Fixing agent was purchased from LiankeBio (China). The 1640 complete medium and FBS were purchased from Gibco (USA). AG490 and DMSO were purchased from SigmaAldrich (USA).
9
Hemin-induced Pheochromocytoma Cell Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Well-differentiated rat pheochromocytoma cells (PC12) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in 1640 complete medium (Gibco, USA) supplemented with 10% foetal bovine serum (FBS, Gibco) with culture conditions of 37°C and a humidified atmosphere of 5 CO 2 . Finally, the cells were exposed to 150 µM hemin for 4 h [22] .
In the experiment, the cells were separated into the control group and the hemin group.
In the experiment, the cells were separated into the control group and the hemin group.
10
Isolation of Rat Splenic Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fresh spleen tissues of rats were placed in 5 mL EP tubes, 2 mL precooled Hank's solution (Camilo Biological, Nanjing, China) was added to it, homogenized by using a homogenizer, and centrifuged at 1200 rpm for 5 min, and the supernatant was discarded. Next, 3 mL erythrocyte lysate was used for resuspended precipitation, and it was allowed to stand for 5 min. Then, 5 mL PBS solution was used to stop the cleavage reaction and centrifuged at 4°C and 1200 rpm for 5 min, and the supernatant was discarded. 5 mL 1640 incomplete medium was used to add resuspended precipitation, which was centrifuged at 1200 rpm for 5 min at 4°C. The precipitation was retained and repeated to wash away the remaining red blood cell lysates. The cells were resuspended with 1640 complete medium (Thermo Fisher Scientific, Waltham, MA, USA), the cell density was adjusted to 2 × 106/100 μL, and they were transferred to a 96-well plate with 100 μL per well. After incubating the IL-4 and IFN-γ antibodies (Life Technology, China) at 4°C in the dark for 30 minutes, 300 μL staining solution was used to resuspend the pellet. Flow cytometry was used to detect cytokines, and the data were analyzed using Flow Jo software. We repeated all the experiments three times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!