Csb e05101m

Manufactured by Cusabio

The CSB-E05101m is a laboratory equipment product manufactured by Cusabio. It is designed to perform a specific function in a research or laboratory setting. A detailed and unbiased description of the product's core function is not available at this time.

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Lab products found in correlation

Csb e12770m, by Cusabio (2 mentions) Csb e05104m, by Cusabio (1 mentions) Csb e06871m, by Cusabio (1 mentions) Csb e13156m, by Cusabio (1 mentions) Fr120, by Toshiba (1 mentions)

4 protocols using csb e05101m

Serum Hormone Profiling by ELISA

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All blood samples were centrifuged at 1,000 g for 10 min and the serum was collected. Concentrations of follicle stimulating hormone (FSH), progesterone (P), luteinizing hormone (LH), and testosterone (T) in serum samples were determined by ELISA kit (CSB-E06871m, CSB-E05104m, CSB-E12770m, CSB-E05101m, CusaBio, Wuhan, China) according to the manufacturer's instructions, and all samples were repeated three times.

Yu Y., Cao Y., Huang W., Liu Y., Lu Y, & Zhao J. (2021). β-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota. Frontiers in Nutrition, 8, 667130.

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Serum Hormone Levels Measured by ELISA

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A mouse estradiol-sensitive ELISA kit (DE4399, Demeditec, Kiel, Germany), a testosterone ELISA kit (csb-E05101m, Cusabio, Wuhan, China), and an FSH ELISA kit (KA2330, Abnova, Taiwan, China) were used to measure the concentrations of estradiol, testosterone, and FSH in the serum according to the manufacturer’s instructions.

Bi W.K., Shao S.S., Li Z.W., Ruan Y.W., Luan S.S., Dong Z.H., Wang J., Wu S.S., Guo T., Ma S.Z., Gao L., Zhao J.J, & He Z. (2020). FSHR ablation induces depression-like behaviors. Acta Pharmacologica Sinica, 41(8), 1033-1040.

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Mouse Hormonal Profile Analysis

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Blood samples were collected by cardiac puncture under anesthesia. Blood samples were centrifuged at 3000 rpm, and serum was separated and stored at −80°C for subsequent blood analysis. ELISA kits were used to measure mouse plasma follicle stimulating hormone (FSH) (CSB-E12770 m, CUSABIO), luteinizing hormone (LH) (CSB-E06871 m, CUSABIO), testosterone (T) (CSB-E05101 m, CUSABIO), anti-Müllerian hormone (AMH) (CSB-E13156 m, CUSABIO), and Irisin (DRE-M9930c, Kmaels) levels.

Zheng Y., He J., Yang D., Yuan M., Liu S., Dai F., Jia Y, & Cheng Y. (2022). Irisin reduces the abnormal reproductive and metabolic phenotypes of PCOS by regulating the activity of brown adipose tissue in mice. Biology of Reproduction, 107(4), 1046-1058.

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Murine Lipoprotein and Testosterone Assay

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After a 4-h fast, blood samples were collected from the retro-orbital sinus puncture in anesthetized mice, and serum was stored at −80°C before analysis. Serum cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and non-esterified fatty acids (NEFAs) were assayed using a biochemical analyzer (FR120; Toshiba, Tokyo, Japan). The serum total and free testosterone levels were assessed by ELISA (CSB-E05101m and CSB-E05098m, Cusabio, Wuhan, China).

Qin N., Zhou Z., Zhao W., Zou K., Shi W., Yu C., Liu X., Dong Z., Mao Y., Liu X., Sheng J., Ding G., Wu Y, & Huang H. (2021). Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer. Frontiers in Cell and Developmental Biology, 9, 637781.

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