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Gapdh clone 6c5

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GAPDH (clone 6C5) is a mouse monoclonal antibody that recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a common housekeeping gene that is involved in glycolysis.

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Lab products found in correlation

Arpc2, by Merck Group (2 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Y 27632, by Merck Group (1 mentions) Ck 666, by Merck Group (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Tamoxifen, by Merck Group (1 mentions) Blebbistatin, by Merck Group (1 mentions) 4 hydroxytamoxifen, by Merck Group (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Vinculin clone hvin 1, by Merck Group (1 mentions) Myosin iib, by Cell Signaling Technology (1 mentions) Myosin iia, by Abcam (1 mentions) Phrodo red avidin, by Thermo Fisher Scientific (1 mentions) P16ink4a clone g175 405, by BD (1 mentions) Horseradish peroxidase conjugated anti mouse or anti rabbit immunoglobulins, by Jackson ImmunoResearch (1 mentions) Lumi lightplus western blotting substrate, by Roche (1 mentions) Las3000 charge coupled device imaging system, by Fujifilm (1 mentions) Phospho nf kb p65, by Cell Signaling Technology (1 mentions) Total p38 mapk, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

GAPDH (1486 citations) GAPDH (6C5, (5 citations)

5 protocols using gapdh clone 6c5

1

Probing Actin Cytoskeleton Dynamics

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Drugs: CK-666 (Sigma), Cytochalasin D (Sigma), Blebbistatin (Sigma), Y-27632 (Sigma); Antibodies: Arpc2 (Millipore), Arp2 (ECM Bio), Arp3 (clone FMS338, Sigma), GAPDH (clone 6C5, Ambion/Life technologies), actin (Clone C4, Millipore), Vinculin (clone hvin1, Sigma), CD11b/aM integrin (Abcam), b1 integrin (Thermo), b2 integrin (Novus), p-Y418 Src Family Kinase (Cell Signaling), p-Tyr (4G10, EMD Millipore), Myosin IIA (Abcam), Myosin IIB (Cell Signaling), p-Erk 1/2 (Cell Signaling), C3 (Abcam), FcγRII/III (Fisher), Src-Family Kinases (Cell Signaling); Chemicals: 4-hydroxy-Tamoxifen (Sigma), Tamoxifen (Sigma), Other: Phalloidin was conjugated to Alexa 488 or Alexa 568 for all experiments (Life Technologies), pHrodo red-avidin (Thermo), biotin-IgG (Rockland), EZ-Link NHS-LC-Biotin (Fisher).
Rotty J.D., Brighton H.E., Craig S.L., Asokan S.B., Cheng N., Ting J.P, & Bear J.E. (2017). Arp2/3 complex is required for macrophage integrin functions but is dispensable for FcR phagocytosis and in vivo motility. Developmental cell, 42(5), 498-513.e6.
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2

Yeast Strain and Plasmid Analysis

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Yeast strains and plasmids are listed in Table S4 and Table S5, respectively. The mig1Δ mig2Δ and mig1Δ mig2Δ mig3Δ yeast strains were a generous gift from Hans Ronne (Swedish University of Agricultural Sciences). Fluorescently labelled Vps4 strains were a gift from David Teis (Innsbruck Medical University). Polyclonal antibodies raised against GFP (Urbanowski and Piper, 1999 (link)) and Rsp5 (Stamenova et al., 2004 (link)), and monoclonal glyceraldehyde 3-phosphate dehydrogenase (GAPDH; clone 6C5; Ambion) and carboxypeptidase Y (CPY; clone 10A5-B5; Molecular Probes) antibodies, were used for immunoblot analysis. These antibodies were routinely used at 1:1000 dilution.
Laidlaw K.M., Bisinski D.D., Shashkova S., Paine K.M., Veillon M.A., Leake M.C, & MacDonald C. (2021). A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast. Journal of Cell Science, 134(2), jcs257733.
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3

Immunoblotting Protein Expression Analysis

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Whole-cell protein extracts were used for analysis, and immunoblotting was conducted as described previously 22 (link). Antibodies against CDK4 (clone D9G3E; Cell Signaling Technology, Danvers, MA), CyclinD1 (clone G124-326; BD Biosciences, Franklin Lakes, NJ), GAPDH (clone 6C5, Ambion), p16INK4a (clone G175-405, BD Biosciences) were used as probes, and horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulins (Jackson Immunoresearch Laboratories, West Grove, PA) were employed as secondary antibodies. The LAS3000 charge-coupled device (CCD) imaging system (Fujifilm Co. Ltd., Tokyo, Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
Nishiwaki M., Toyoda M., Oishi Y., Ishida S., Horiuchi S.I., Makino-Itou H., Kimura T., Ohno S.I., Ohkura T., Enosawa S., Akutsu H., Nakazawa A., Kasahara M., Kiyono T, & Umezawa A. (2020). Immortalization of human hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing. Scientific Reports, 10, 17503.
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4

Comprehensive Immunoblotting Assay Panel

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GAPDH (clone 6C5) (ThermoFisher, Waltham, MA, USA; #AM4300); Myosin IIA (Cell Signaling, Danvers, MA, USA; #3403S); iNOS (abcam, Cambridge, UK; #ab15323); Phospho-P65 NF-kB (Cell Signaling, Danvers, MA, USA; #3033S); Total p65 (Cell Signaling, Danvers, MA, USA; #8242); Phospho-p38 MAPK (Cell Signaling, Danvers, MA, USA; #9211); Total p38 MAPK (Cell Signaling, Danvers, MA, USA; #9212); Phospho-P44/P42 MAPK (ERK 1/2) (Cell Signaling, Danvers, MA, USA; #9106); Total p44/p42 MAPK (ERK 1/2) (Cell Signaling, Danvers, MA, USA; #4695); IkBa (Cell Signaling, Danvers, MA, USA; #9242); Arpc2 (Millipore, Burlington, MA, USA; #07-227); MHCII (clone M5/114.15.2) (Biolegend, San Diego, CA, USA; #107601); APC-conjugated MHCII (clone M5/114.15.2) (Biolegend, San Diego, CA, USA; #107613).
Ronzier E., Laurenson A.J., Manickam R., Liu S., Saintilma I.M., Schrock D.C., Hammer J.A, & Rotty J.D. (2022). The Actin Cytoskeleton Responds to Inflammatory Cues and Alters Macrophage Activation. Cells, 11(11), 1806.
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5

Antibody Sources for Protein Detection

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Antibodies were purchased from BD Transduction Laboratories (Heidelberg, Germany; E-cadherin clone 36; IF 1:100, IB 1:1000), Sigma-Aldrich (Munich, Germany; AJAP1 HPA012157, IF 1:100, WB 1:1000; SMA clone 1A4, IF 1:100; pan-cadherin CH-19, IB 1:1000; myc C3956, IF 1:100, IB 1:1000), Ambion (Darmstadt, Germany; GAPDH clone 6C5, IB 1:10,000), Thermo scientific (Darmstadt, Germany; Grp94 clone 9G10.F8.2, IB 1:500), Abcam (Cambridge, UK; AJAP1 ab121361, IF 1:100, IB 1:1000), R&D Systems (Wiesbaden, Germany; AJAP1 AF7970), AntibodyVerify (Las Vegas, NV, USA; AJAP1 AAS47449C), Santa Cruz (Heidelberg, Germany; β-catenin H102, IB 1:1000; β-casein M-14, IB 1:1000) and custom made antibodies against shrew-1 (Nanotools, IB 1:500, polyclonal Genovac and monoclonal Genovac clone F, IF 1:50 and IB 1:500). Secondary antibodies (Alexa Fluor 488-, 594- and 647-labeled antibodies, IF 1:400) were purchased from Molecular Probes (Leiden, The Netherlands) or Abcam (Cambridge, UK) and Horseradish peroxidase (HRP)-conjugated secondary antibody from Jackson Immuno Research (Dianova, Hamburg, Germany) or Biosource (Camarillo, CA, USA).
Klemmt P.A., Resch E., Smyrek I., Engels K., Stelzer E.H, & Starzinski-Powitz A. (2016). Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile. Biology Open, 5(11), 1607-1619.
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