K2edta map tubes

Prior to drawing blood samples, the CVC was flushed with saline from a 10 mL PosiFlush saline syringe (BD Biosciences, San Jose, CA, USA). Blood samples were collected daily from each NHP’s CVC between days 3 through 16 post-exposure with a 5 mL syringe and aliquoted into 0.5 mL K2EDTA MAP tubes (BD Biosciences), 1 mL serum with clot activator tubes, and 1 mL sodium citrate MiniCollect tubes (Greiner Bio-One, Monroe, NC, USA). After day 16, blood was collected from the survivor on day 30 and 41. The CVC line was again flushed with saline and maintained by flushing with PosiFlush pre-filled heparin lock syringes (BD Biosciences), which were left attached to the CVC until the next sample collection. On days where sampling via CVC was complicated due to kinking of the line, NHPs were anesthetized with ketamine HCl at 10 mg/kg (VedCo, Saint Joseph, MO, USA) or a combination of Tiletamine HCl and Zolazepam HCl at 3 mg/kg (Fort Dodge Animal Health, New York, NY, USA). Survivor and D10:Non-survivor had catheter kinking three days prior to challenge; no other kinks are noted. Once anesthetized, blood was drawn via saphenous venipuncture.

CVCs were flushed with saline from a 10 mL PosiFlush saline syringe (BD Biosciences, San Jose, CA, USA) prior to blood draw. The daily collection of blood samples from each CVC occurred between DPE-3 and DPE 10 with a 2.5 mL volume collected with a 5 mL syringe distributed into 1 mL sodium citrate MiniCollect tubes (Greiner Bio-One, Monroe, NC, USA), 1 mL clot activator tubes (BD Biosciences), and 0.5 mL K2EDTA MAP tubes. Saline was used to again flush the CVC line, which was then maintained by flushing with PosiFlush pre-filled heparin lock syringes (BD Biosciences). These syringes were left attached to the CVC cage-side port until the next sample collection. Animals were anesthetized with ketamine HCl at 10 mg/kg (VedCo, Saint Joseph, MO, USA) or a combination of tiletamine HCl and zolazepam HCl at 3 mg/kg (Fort Dodge Animal Health, New York, NY, USA) on days where sampling through the CVC was complicated due to kinked lines. When animals were anesthetized, blood was drawn via the saphenous vein.