Antibodies used in this work were as follows: myc (05‐724; 1:200 IF, 1:2,000 WB) monoclonal antibody (Millipore); DAB2 (sc‐13982; 1:200 IF, 1:2,000 WB),
APPL1 (sc‐67402; 1:100 IF, 1:1,000 WB), SH3BP4 (sc‐393730; 1:50 IF, 1:500 WB), LARG (sc‐25638; 1:50 IF),
SEPT7 (sc‐20620, 1:50 IF, 1:1,000 WB) and EF2 (sc‐13004; 1:2,000 WB) antibodies (Santa Cruz); GIPC1 (25‐6792; 1:100 IF, 1:1,000 WB) polyclonal antibody (Proteus);
GAPDH (G8795; 1:10,000 WB) monoclonal antibody (Sigma); HA (11867423001, 1:200 IF) antibody (Roche);
GFP (A11122; 1:400 IF) polyclonal antibody (Life Technologies); GFP (ab1218; 1:400 IF) monoclonal antibody (Abcam); affinity‐purified rabbit polyclonal antibodies against GFP (1:2,000 WB) and MYO6 (1:100 IF, 1:1,000 WB) were generated as described previously
45. Affinity‐purified rabbit polyclonal antibodies against amino acids 383–648 of LRCH3 (1:1,000 WB) or amino acids 1500–2140 of DOCK7 (1:1,000 WB) were generated as part of this work.
O'Loughlin T., Masters T.A, & Buss F. (2018). The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics. EMBO Reports, 19(4), e44884.
