Laminin, poly‐ornithine, thyroid hormone (T3), paraformaldehyde (PFA), bis(cyclohexanone)oxaldihydrazone (Cuprizone), Hoechst 33342, poly‐d ‐lysine, papain, l ‐cysteine, insulin, transferrin, progesterone, putrescine, BSA, and 5‐fluoro‐2′‐deoxyuridine were purchased from Sigma‐Aldrich. Collagenase A was purchased from Roche. EGF, bFGF, and PDGF‐AA were purchased from Peprotech. PD0325901, CI‐1040, AZD8330, and AZD6244 were purchased from MedChemExpress, and U0126 was purchased from Tocris.
Thyroid hormone
Manufactured by Merck Group
Sourced in United Kingdom, United States
Thyroid hormone is a laboratory product that measures the levels of thyroid hormones, such as thyroxine (T4) and triiodothyronine (T3), in the body. It is used to assess thyroid function and diagnose conditions related to the thyroid gland.
Automatically generated - may contain errors
Lab products found in correlation
Putrescine, by Merck Group (2 mentions)
Progesterone, by Merck Group (2 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions)
Pdgf aa, by Thermo Fisher Scientific (2 mentions)
Insulin, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Neurobasal medium, by Thermo Fisher Scientific (2 mentions)
Cuprizone, by Merck Group (1 mentions)
Laminin, by Merck Group (1 mentions)
Ci 1040, by MedChemExpress (1 mentions)
Pd0325901, by MedChemExpress (1 mentions)
Azd8330, by MedChemExpress (1 mentions)
Azd6244, by MedChemExpress (1 mentions)
U0126, by Bio-Techne (1 mentions)
5 fluoro 2 deoxyuridine, by Merck Group (1 mentions)
Poly ornithine, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Poly d lysine, by Merck Group (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Papain, by Merck Group (1 mentions)
7 protocols using thyroid hormone
1
Protocols for Neural Stem Cell Culture
2
Myelination of DRG Neurons by aMSC-Derived Oligodendrocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dorsal root ganglia of embryonic day 15 rats were dissociated with TrypLE Express and collected by centrifugation (250× g, 5 min, 4 °C) [16 (link),17 (link),18 (link),19 (link),20 ,21 (link)]. Cells were resuspended in neuron maintenance medium comprising Neurobasal medium (Thermo Fisher Scientific) supplemented with 2% B27 and NGF (20 ng/mL, Millipore, Burlington, MA, USA) and then seeded at 100,000 cells/cm2 onto poly-d -lysine/laminin-coated plates. Endogenous Schwann cells and fibroblasts were eliminated following brief treatment with fluorodeoxyuridine and uridine (10 μM each, Sigma-Aldrich), whereas dorsal root ganglion neurons and neurite network remained adherent on the coated plates.
aMSC-derived OPs were seeded at 10,000 cells/cm2 onto the axonal network of dorsal root ganglion neurons and the co-culture was maintained in myelination medium comprising Neurobasal medium supplemented with B27, NGF neutralizing antibody (5 μg/mL, Abcam, Cambridge, UK), thyroid hormone (10 ng/mL, Sigma-Aldrich), and DAPT (10 ng/mL, Sigma) for 14 days. Myelin-forming OLs and axons in co-cultures were immunostained for myelin basic protein (MBP) and neurofilament 200 (NF200) respectively. Co-cultures were also prepared for examination of compact myelin under transmission electron microscopy. aMSCs in co-culture with dorsal root ganglion neurons were prepared in parallel as controls.
aMSC-derived OPs were seeded at 10,000 cells/cm2 onto the axonal network of dorsal root ganglion neurons and the co-culture was maintained in myelination medium comprising Neurobasal medium supplemented with B27, NGF neutralizing antibody (5 μg/mL, Abcam, Cambridge, UK), thyroid hormone (10 ng/mL, Sigma-Aldrich), and DAPT (10 ng/mL, Sigma) for 14 days. Myelin-forming OLs and axons in co-cultures were immunostained for myelin basic protein (MBP) and neurofilament 200 (NF200) respectively. Co-cultures were also prepared for examination of compact myelin under transmission electron microscopy. aMSCs in co-culture with dorsal root ganglion neurons were prepared in parallel as controls.
3
Isolation and Culture of O4+ OPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cortices from P6-P7 pups (seven pooled biological replicates per genotype, mixed sex) were dissected and subsequently dissociated with the Neural Tissue Dissociation Kit from Miltenyi. Anti-O4 magnetic microbeads (Miltenyi) were then used to isolate a pure population of O4+ OPCs. For culture and differentiation, serum- and antibiotic-free medium comprising 2% B27 (Gibco) and 30 ng/ml thyroid hormone (Sigma) in Neural Basal Media (Gibco) was used. Medium was changed daily and cells were harvested after 7 days.
4
In Vitro Leydig Cell Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For LCs lineage differentiation, the SLCs were plated in fresh differentiation-inducing medium containing phenol red-free DMEM/F12 (Gibco), 2% FBS (HyClone), 1 ng/mL luteinizing hormone (LH, R&D, Minneapolis, MN, USA), 1 nM thyroid hormone (Sigma-Aldrich), 10 ng/mL platelet-derived growth factor-AA (PDGF-AA, PeproTech), 50 ng/mL insulin-like growth factor 1 (IGF1, PeproTech), and 1% ITS (Gibco) and incubated for up to 28 days as previously described 52 (link). Cell culture supernatants were collected for testosterone detection at the indicated time points. Differentiation was confirmed by immunostaining and RT-PCR for LCs lineage markers (primers and antibodies are listed in Table S4 -5).
5
Oligodendrocyte Progenitor Cell Isolation and Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolated OPCs were seeded into 96 well-plates (InVitro-Sciences) coated with PDL (Sigma). After isolation, OPCs were left to recover in OPC medium (60µg/ml N-Acetyl cysteine (Sigma), 10µg/ml human recombinant insulin (Gibco), 1mM sodium pyruvate (Gibco), 50µg/ml apo-transferrin (Sigma), 16.1µg/ml putrescine (Sigma), 40ng/ml sodium selenite (Sigma), 60ng/ml progesterone (Sigma), 330µg/ml bovine serum albumin (Sigma)) supplemented with b-FGF and PDGF (30ng/ml each, Peprotech). OPCs were incubated at 37 º C, 5% CO 2 and 5% O 2 . The medium was completely exchanged to OPC medium with 20ng/ml bFGF and PDGF after overnight culture to remove any dead cells. After 3d the cell culture medium was switched to promote further proliferation (OPC medium+20ng/ml bFGF and PDGF) or differentiation (OPCM + 40ng/ml T3). During differentiation or proliferation experiments 66% of the medium was replaced every 48h and growth factors or other small molecules were added fresh to the culture. The culture medium used was 100μl for cultures in 96 well plate wells. For proliferation and differentiation assays the medium was in some instances supplemented with 40ng/ml thyroid-hormone (T3, Sigma) and varying concentrations of nocodazole (Sigma, M1404).
6
Isolation and Culture of Erythroblasts from Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Red blood cells were isolated from heparinized venous blood by centrifugation at 210g at room temperature for 15 min. Cells were resuspended in SAGM medium and analyzed on an ADVIA 2120 Hematology System (Bayer Healthcare AG, Leverkusen, Germany). Peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation using Percoll (GE Healthcare) with density ρ = 1.077. Cells were cultured in StemSpan medium (Stem Cell Technologies, Grenoble, France) supplemented with 2 U/mL erythropoietin (Epo, Eprex, Janssen-Cilag, Tilburg, Netherlands), 100 ng/mL stem cell factor (SCF), 1 μmol/L dexamethasone (Sigma) 50 μg/mL cholesterolrich lipid mix (Sigma), and 10 mL/L penicillin/streptomycin (PAA Laboratories, Pasching, Austria). After 5 days, erythroblasts were isolated by density centrifugation using Percoll with density ρ = 1.075 and kept in culture at 2 × 10 6 cells/mL. Differentiation was initiated by culturing the cells in StemSpan medium supplemented with 10 U/mL Epo, 1 mg/mL holotransferrin (Scipac, Sittingbourne, UK), 10 ng/mL insulin (Sigma), 1 μmol/L thyroid hormone (Sigma), 3% human AB serum (Sigma), and 10 mL/L penicillin/streptomycin.
7
Nanoparticle-based Breast Cancer Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NDAT was synthesized as previously described [20] (link). Thyroid hormone and resveratrol were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). resveratrol was prepared as a stock concentration of 100 nM in dimethyl sulfoxide (DMSO). NDAT and T 4 were prepared as stock concentrations of 10 -2 M in PBS and KOH-propylene glycol, respectively. Reagents were conserved at -20 °C. The final concentrations of solvents used to dissolve the reagents were tested for activity and did not affect the results of the experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!