Rnase h
RNase H is an enzyme that selectively degrades the RNA strand in RNA-DNA hybrids. It plays a crucial role in various molecular biology techniques involving the manipulation of nucleic acids.
Lab products found in correlation
26 protocols using rnase h
U1 snRNA-Binding Protein Analysis
RNA Oligonucleotide Hybridization and Enzymatic Cleavage
Interferon and Inflammation Gene Expression Analysis
Primer probes used were IFNB1 (Hs01077958_s1); IL1A (Hs00174092_m1); IL6 (Hs00985639_m1); NFKB1 (Hs00765730_m1); NFKB2 (Hs010208901); STAT1 (Hs01013996_m1); STAT2 (Hs01013123_m1); IRF7 (Hs01014809_g1); GAPDH (Hs02758991_g1) (all Applied Biosystems).
Mapping 18S rRNA Cleavage Sites
3′ RACE analysis was adapted from Kiss and Filipowicz (38 (link)). Primers ITS1-Hs-RACE (5′- CGCGAATTCGATCATTAACGGAGCCCGGAG-3′) and ITS1+2-Hs-RACE (5′-CGCGAATTCACCTGCGGAAGGATCATTAAC-3′), which spans the 18S-ITS1 junction up to nucleotide 13 and to nucleotide 2 in the ITS1 respectively, were used for PCR amplification. The amplified fragments were sub-cloned, automatically sequenced and aligned with Jalview software (39 (link)).
Mouse Gene Expression Analysis
Quantitative RT-PCR Analysis of Rat Neuroendocrine Genes
Treating Fractions with D-Loop Structures
Quantitative Real-Time PCR Protocol
RNA-Seq Library Preparation Protocol
Measuring PABPC1 Poly(A) Tail Length
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