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Ab105767

Manufactured by Merck Group
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Ab105767 is a laboratory equipment product. It is a device used for measuring and analyzing various samples in a controlled environment. The core function of this product is to provide accurate and reliable data for research and laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab137027, by Abcam (2 mentions) Ab290, by Abcam (2 mentions) Amersham hybond, by GE Healthcare (2 mentions) Protran, by GE Healthcare (2 mentions) Labtek 2 chambered coverslips, by Thermo Fisher Scientific (2 mentions) Mab1501, by Abcam (2 mentions) Nupage bis tris gradient gel, by Thermo Fisher Scientific (2 mentions) Ab9485, by Abcam (2 mentions)

2 protocols using ab105767

1

Western Blotting of ESCRT Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded and transfected as described above in LabTek II chambered coverslips (ThermoScientific). Protein extracts were separated on 4-12% NuPAGE Bis-Tris gradient gels (Life Technologies) and transferred to Nitrocellulose (Protran, GE Healthcare) or PVDF membranes (Amersham Hybond, GE Healthcare). Western blotting was performed by standard methods using antibodies against CHMP4B (1:1000, Abcam, ab105767), actin (1:30000, Merck Millipore, MAB1501), VPS4B (1:500, Abcam, ab137027), GFP (1:5000, Abcam, ab290) and GAPDH (1:2500, Abcam, ab9485).
Mierzwa B.E., Chiaruttini N., Redondo-Morata L., von Filseck J.M., König J., Larios J., Poser I., Müller-Reichert T., Scheuring S., Roux A, & Gerlich D.W. (2017). Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nature cell biology, 19(7), 787-798.
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2

Western Blotting of ESCRT Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded and transfected as described above in LabTek II chambered coverslips (ThermoScientific). Protein extracts were separated on 4-12% NuPAGE Bis-Tris gradient gels (Life Technologies) and transferred to Nitrocellulose (Protran, GE Healthcare) or PVDF membranes (Amersham Hybond, GE Healthcare). Western blotting was performed by standard methods using antibodies against CHMP4B (1:1000, Abcam, ab105767), actin (1:30000, Merck Millipore, MAB1501), VPS4B (1:500, Abcam, ab137027), GFP (1:5000, Abcam, ab290) and GAPDH (1:2500, Abcam, ab9485).
Mierzwa B.E., Chiaruttini N., Redondo-Morata L., von Filseck J.M., König J., Larios J., Poser I., Müller-Reichert T., Scheuring S., Roux A, & Gerlich D.W. (2017). Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nature cell biology, 19(7), 787-798.
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