Log In Sign up
The largest database of trusted experimental protocols

Twincam adaptor

Manufactured by Cairn Research
Visit Supplier
Sourced in United Kingdom

The TwinCam adaptor is a device designed to connect two cameras to a microscope or other optical system. It allows for simultaneous image capture from two different cameras, enabling concurrent observation and analysis of samples.

Automatically generated - may contain errors

Lab products found in correlation

Az100 microscope, by Nikon (4 mentions) Orcaflash4.0 v2 digital cameras, by Hamamatsu Photonics (3 mentions) Ff458dio2 dichroic, by IDEX Corporation (2 mentions) Orca flash4.0 v2, by Hamamatsu Photonics (1 mentions) Lambda ls xenon arc lamp, by Sutter Instruments (1 mentions)

Close spelling variants of this product

TwinCam (2 citations)

4 protocols using twincam adaptor

1

Monitoring Neuronal Ca2+ Dynamics in C. elegans

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Aβ42 and mCherry control lines were crossed with strain AX2073 expressing Ca2+ sensor YC3.60 [42 (link)] under the flp-17 promoter, driving expression in BAG. The assay was performed on freely moving animals using the set-up described previously [43 (link)]. Individual animals were placed on 5 cm agarose plates (17 g/L agarose, 3 g/L NaCl, 5 mg/L cholesterol, 1 mM MgSO4, 1 mM CaCl2) seeded with a 3 μL concentrated drop of E. coli OP50. A PDMS chamber was placed on top and the gases were applied at 1.4 mL/min, with 21% O2 for 2 min, 21% O2 + 3% CO2 for 2 min, and finally 21% O2 for 2 min.
Videos were recorded at 10 frames per second using 100 ms exposure time on a Nikon AZ100 microscope with an AZ Plan Fluor 2x lens. A TwinCam adaptor (Cairn Research, UK) and two ORCA-Flash4.0 V2 digital cameras (Hamamatsu, Japan) were used to simultaneously record YFP and CFP fluorescence. The neurons were tracked and the YFP/CFP ratios (R) calculated using custom-written Matlab software. R0 was defined as the initial value averaged over the first 100 frames (10 s) and ΔR/R0 expressed as the percentage increase.
Sinnige T., Ciryam P., Casford S., Dobson C.M., de Bono M, & Vendruscolo M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates the associated behavioural response. PLoS ONE, 14(5), e0217746.
+ Open protocol
+ Expand
2

Imaging CO2-Evoked Calcium Signaling in BAG Neurons

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals expressing a flp-17p::YC3.60 (yellow cameleon 3.60) transgene were used for ratiometric imaging of relative calcium concentration in BAG cell bodies (Bretscher et al., 2011 (link)). After immobilization, animals were placed under a microfluidic PDMS chamber and exposed to a 0% CO2 (3 min) - X% CO2 (3 min) - 0% CO2 (3 min) stimulus train, with X corresponding to 1%, 3% or 5% CO2 depending on the experiment. To measure CO2-evoked tonic Ca2+ activity in BAG, the time interval for CO2 stimulation was prolonged from 3 min to 18 min. In all experiments, the background O2 level was 7% O2. Calcium imaging was done at 2 frames/s on an AZ100 microscope (Nikon) bearing a TwinCam adaptor (Cairn Research) mounted with two ORCAFlash4.0 V2 digital cameras (Hamamatsu) using an AZ Plan Fluor 2x lens with 2x zoom and an exposure time of 500 ms.
Beets I., Zhang G., Fenk L.A., Chen C., Nelson G.M., Félix M.A, & de Bono M. (2020). Natural Variation in a Dendritic Scaffold Protein Remodels Experience-Dependent Plasticity by Altering Neuropeptide Expression. Neuron, 105(1), 106-121.e10.
+ Open protocol
+ Expand
3

Imaging Neuronal Ca2+ Activity in Transgenic C. elegans

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five to ten young adult transgenic animals (<24 hr old) expressing the YC2.60 (URX) or the YC3.60 (AQR and PQR) Ca2+ sensors were glued to agarose pads (2% in M9 buffer, 1 mM CaCl2) using Dermabond tissue adhesive, with their body immersed in OP50 washed off from a seeded plate using M9. The animals were quickly covered with a PDMS microfluidic chamber and 7% O2 pumped into the chamber for 2 min before imaging, to allow animals to adjust to the new conditions. Neural activity was recorded for 6 min with switches in O2 concentration every 2 min. Imaging was on an AZ100 microscope (Nikon) equipped with a TwinCam adaptor (Cairn Research), two ORCAFlash4.0 V2 digital cameras (Hamamatsu), and an AZ Plan Fluor 2x objective with 2x zoom. Recordings were at 2 frame-per-second (fps) with a 500ms exposure time. Excitation light from a C-HGFI Intensilight lamp (Nikon) was passed through a 438/24 nm filter and an FF458-DiO2 dichroic (Semrock). Emitted light was passed to a DC/T510LPXRXTUf2 dichroic filter in the TwinCam adaptor cube and then through 483/32 nm (CFP) or 542/27 nm (YFP) filters before collection on the cameras.
Valperga G, & de Bono M. (2022). Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans. eLife, 11, e68040.
+ Open protocol
+ Expand
4

Imaging Calcium Activity in C. elegans

Check if the same lab product or an alternative is used in the 5 most similar protocols
To image young adults, we picked L4 animals expressing the YC2.60 Ca2+ sensor 24 hr before imaging. On the day of the assay, 5 – 10 day 1 adult animals were glued to agarose pads (2% in M9 buffer, 1 mM CaCl2), using Dermabond tissue adhesive, with their body immersed in OP50 washed off from a seeded plate using M9 buffer. The animals were quickly covered with a PDMS microfluidic chamber and 7% O2 was pumped into the chamber for 2 min before we began imaging, to allow animals to adjust to the new conditions. Neural activity was recorded for 9 min with switches in O2 concentration every 2 min.
Imaging was performed on an AZ100 microscope (Nikon) bearing a TwinCam adaptor (Cairn Research, UK), two ORCAFlash4.0 V2 digital cameras (Hamamatsu, Japan), and an AZ Plan Fluor 2× lens with 2× zoom. Recordings were at 2Hz. Excitation light from a Lambda LS xenon arc lamp (Sutter) was passed through a 438/24 nm filter and an FF458DiO2 dichroic (Semrock). Emitted light was passed to a DC/T510LPXRXTUf2 dichroic filter in the TwinCam adaptor cube and then filtered using a 483/32 nm filter (CFP), or 542/27 nm filter (YFP) before collection on the cameras. Recordings were analyzed using Neuron Analyzer, a customwritten Matlab program available at https://github.com/neuronanalyser/neuronanalyser.
Cohn J., Dwivedi V., Valperga G., Zarate N., de Bono M., Horvitz H.R, & Pierce J.T. (2019). Activity-Dependent Regulation of the Proapoptotic BH3-Only Gene egl-1 in a Living Neuron Pair in Caenorhabditis elegans. G3: Genes|Genomes|Genetics, 9(11), 3703-3714.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!