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Bioanalyzer 6000 pico kit

Manufactured by Agilent Technologies
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The Bioanalyzer 6000 Pico Kit is a lab equipment product from Agilent Technologies. It is designed for the analysis of small sample sizes, providing high-resolution data on the size and concentration of nucleic acid samples.

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Lab products found in correlation

Rneasy micro kit, by Qiagen (2 mentions) Pen membrane glass slides, by Thermo Fisher Scientific (2 mentions) Dm lmd 6000 laser microdissection system, by Leica (2 mentions) Rneasy micro kit, by Agilent Technologies (2 mentions) Allprep dna rna protein mini kit, by Qiagen (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Rneasy mini spin column, by Qiagen (1 mentions) Qiazol, by Qiagen (1 mentions) Dna 1000 kit, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Bioanalyzer (3289 citations) Bioanalyzer RNA 6000 Pico Kit (32 citations) Agilent Bioanalyzer RNA 6000 Pico Kit (14 citations) 2100 Bioanalyzer RNA 6000 Pico kit (11 citations) Bioanalyzer Pico Kit (4 citations) Agilent 2100 Bioanalyzer RNA 6000 Pico Kit (4 citations) Agilent RNA 6000 Pico Kit on Bioanalyzer (2 citations)

4 protocols using bioanalyzer 6000 pico kit

1

Laser Capture Microdissection of Fetal Cerebellum

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Whole cerebellum was dissected from 16 fetal specimens that had intact calvaria to ensure correct orientation of the cerebellum. Intact cerebella were embedded in OCT, frozen at −80°C, and cryosectioned at 16-μm in the sagittal plane through the cerebellar vermis onto PEN Membrane Glass Slides (Applied Biosystems, USA). Total RNA was isolated from one whole section using the Qiagen RNeasy Micro Kit and RNA quality was assessed using the Agilent Bioanalyzer 6000 Pico Kit before proceeding with LCM. LCM was performed using the Leica DM LMD-6000 Laser Microdissection system to capture tissue containing PCL and EGL from each of 6–8 sections per slide into separate collection tubes. Total RNA was then isolated from LCM-enriched samples pooled across 9 slides using the Qiagen RNeasy Micro Kit. LCM was previously performed to capture RL ventricular (RLVZ) and subventricular zones (RLSVZ), then total RNA was isolated from RLVZ and RLSVZ resulting in two RNA samples per specimen.10 (link)
Aldinger K.A., Thomson Z., Phelps I.G., Haldipur P., Deng M., Timms A.E., Hirano M., Santpere G., Roco C., Rosenberg A.B., Lorente-Galdos B., Gulden F.O., O’Day D., Overman L.M., Lisgo S.N., Alexandre P., Sestan N., Doherty D., Dobyns W.B., Seelig G., Glass I.A, & Millen K.J. (2021). Spatial and cell-type transcriptional landscape of human cerebellar development. Nature neuroscience, 24(8), 1163-1175.
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2

Laser Capture Microdissection of Fetal Cerebellum

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Whole cerebellum was dissected from 16 fetal specimens that had intact calvaria to ensure correct orientation of the cerebellum. Intact cerebella were embedded in OCT, frozen at −80°C, and cryosectioned at 16-μm in the sagittal plane through the cerebellar vermis onto PEN Membrane Glass Slides (Applied Biosystems, USA). Total RNA was isolated from one whole section using the Qiagen RNeasy Micro Kit and RNA quality was assessed using the Agilent Bioanalyzer 6000 Pico Kit before proceeding with LCM. LCM was performed using the Leica DM LMD-6000 Laser Microdissection system to capture tissue containing PCL and EGL from each of 6–8 sections per slide into separate collection tubes. Total RNA was then isolated from LCM-enriched samples pooled across 9 slides using the Qiagen RNeasy Micro Kit. LCM was previously performed to capture RL ventricular (RLVZ) and subventricular zones (RLSVZ), then total RNA was isolated from RLVZ and RLSVZ resulting in two RNA samples per specimen.10 (link)
Aldinger K.A., Thomson Z., Phelps I.G., Haldipur P., Deng M., Timms A.E., Hirano M., Santpere G., Roco C., Rosenberg A.B., Lorente-Galdos B., Gulden F.O., O’Day D., Overman L.M., Lisgo S.N., Alexandre P., Sestan N., Doherty D., Dobyns W.B., Seelig G., Glass I.A, & Millen K.J. (2021). Spatial and cell-type transcriptional landscape of human cerebellar development. Nature neuroscience, 24(8), 1163-1175.
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3

Rapid Subcutaneous Fat Tissue Sampling

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Samples were snap frozen immediately after collection in liquid nitrogen and then stored at -80°C until extraction. DNA and RNA were isolated from subcutaneous fat tissue after rapid thaw at 37°C with the AllPrep DNA/RNA/protein Mini kit (QIAGEN, Valencia, CA). DNA was stored in TE buffer, quantified and checked for quality on a Nanodrop-1000 (Nanodrop, Wilmington, DE) and stored at -80°C. RNA samples were quantified and checked for quality using the BioAnalyzer 6000 Pico kit (Agilent, Santa Clara, CA) and stored at -80°C.
Jones M.R., Brower M.A., Xu N., Cui J., Mengesha E., Chen Y.D., Taylor K.D., Azziz R, & Goodarzi M.O. (2015). Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity. PLoS Genetics, 11(8), e1005455.
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4

Extracellular Vesicle miRNA Profiling in T1D

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Type 1 diabetes, 10yT1D, and HC as well as TX plasma enriched EVs precipitated fractions were resuspended in 200 μL PBS and dissolved in 1 mL Qiazol (Qiagen, 79306). After chloroform-Qiazol centrifugation and aqueous layer phase separation, RNA was isolated following a manufacture’s procedure using RNeasy Mini Spin Columns (Qiagen), cleaned with RWT, RPE buffers (Qiagen) and eluted in nuclease free water. Isolated RNA size distribution profile and concentration was assessed on Bioanalyzer 2100 using Bioanalyzer 6000 Pico kit (Agilent, 5067–1514) and stored at −80°C before further processing. Small non-coding RNA EVs libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, E7300S) following standard protocol without size selection step. NGS libraries were checked for QC using DNA 1000 kit on Bioanalyzer 2100 (Agilent, 5067-1505) and quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630). Small RNA libraries were pooled in equimolar ratio. EVs plasma miRNA samples were sequenced with 150 M raw sequencing reads for nT1D, 10yT1D, HC, and TX samples on Illumina Hiseq.
Tesovnik T., Kovač J., Pohar K., Hudoklin S., Dovč K., Bratina N., Trebušak Podkrajšek K., Debeljak M., Veranič P., Bosi E., Piemonti L., Ihan A, & Battelino T. (2020). Extracellular Vesicles Derived Human-miRNAs Modulate the Immune System in Type 1 Diabetes. Frontiers in Cell and Developmental Biology, 8, 202.
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